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Photosynthesis research protocols

Author: Robert Carpentier
Publisher: New York, N.Y. : Humana Press, ©2011.
Series: Springer protocols (Series); Methods in molecular biology (Clifton, N.J.), volume 684.
Edition/Format:   eBook : Document : English : 2nd edView all editions and formats
Summary:
The capture of sunlight by photosynthetic organisms supplies an enormous amount of the energy required to develop and sustain life on the planet, making photosynthesis one of the most important biological phenomenon on Earth. Updating the popular first edition, Photosynthesis Research Protocols, Second Edition presents detailed descriptions of a broad range of general and fundamental methods that are commonly used  Read more...
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Genre/Form: Electronic books
Laboratory manuals
Additional Physical Format: Print version:
Photosynthesis research protocols.
New York, N.Y. : Humana Press, ©2011
(OCoLC)671806296
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Robert Carpentier
ISBN: 9781607619253 1607619253
OCLC Number: 680490599
Language Note: English.
Description: 1 online resource (xiv, 395 pages) : illustrations (some color).
Contents: Isolation of photosystem II-enriched membranes and the oxygen-evolving complex subunit proteins from higher plants --
Isolation of photosystem I submembrane fractions --
Isolation of photosystem II reaction center complexes from plants --
Methods for the isolation of functional photosystem II core particles from the cyanobacterium Synechocystis sp. PCC 6803 --
Purification and crystallization of oxygen-evolving photosystem II core complex from thermophilic cyanobacteria --
Isolation of cytochrome b6f complex from grana and stroma membranes from spinach chloroplasts --
Purification and crystallization of the cyanobacterial cytochrome B6F complex --
Purification of plastocyanin and cytochrome C6 from plants, green algae, and cyanobacteria --
Isolation and identification of chloroplast lipids --
Isolation and purification of CP43 and CP47 photosystem II proximal antenna complexes from plants --
Preparation of native and recombinant light-harvesting chlorophyll-a/b complex --
Isolation and characterization of lamellar aggregates of LHCII and LHCII-lipid macro-assemblies with light-inducible structural transitions --
Protein targeting across and into chloroplast membranes --
Proteomic analysis of thylakoid membranes --
Thylakoid phosphoproteins: Identification of phosphorylation sites --
Direct detection of free radicals and reactive oxygen species in thylakoids --
Assay of photoinhibition and heat inhibition of photosystem II in higher plants --
Photosystem II reconstitution into proteoliposomes and methodologies for structure-function characterization --
Physical and chemical immobilization methods of photosynthetic materials --
Identifying chloroplast biogenesis and signalling mutants in arabidopsis thaliana --
Expression of genes in cyanobacteria: Adaptation of endogenous plasmids as platforms for high-level gene expression in synechococcus sp. PCC 7002 --
Construction of gene interruptions and gene deletions in the cyanobacterium synechocystis sp. Strain PCC 6803 --
A simple method for chloroplast transformation in Chlamydomonas reinhardtii --
Rapid isolation of intact chloroplasts from spinach leaves --
Mechanical isolation of bundle sheath cell strands and thylakoids from leaves of C4 grasses --
Isolation of ribulose-1,5-bisphosphate carboxylase/oxygenase from leaves --
Quantifying the amount and activity of Rubisco in leaves --
Purification of Rubisco activase from leaves or after expression in escherichia coli --
Rubisco activase activity assays --
Quantification of Rubisco activase content in leaf extracts.
Series Title: Springer protocols (Series); Methods in molecular biology (Clifton, N.J.), volume 684.
Responsibility: edited by Robert Carpentier.

Abstract:

Now in a new and fully updated edition, this book presents detailed descriptions of a plethora of general and fundamental photosynthesis methods used by plant biochemists, physiologists, and  Read more...

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From the reviews of the second edition:"Photosynthesis research protocols focuses on various aspects of photosynthesis research and its chapters reflect the whole complexity of this research field. Read more...

 
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