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Plant cell wall analysis

Author: H F Linskens; J F Jackson; A Bacic
Publisher: Berlin ; London : Springer, ©1996.
Series: Modern methods of plant analysis, v. 17.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
This book brings together techniques dealing with those parts of plant cells which have often been thought of as being extremely difficult to analyse. Cell wall porosity adhesives, proteins, chitin, lignin, oligosaccharides, pectins and other polymers are all the subject of analytical procedures in this volume. Wherever possible, the methods stand alone with little or no necessity to consult other works to carry out  Read more...
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Genre/Form: Electronic books
Additional Physical Format: Print version:
Plant cell wall analysis.
Berlin ; London : Springer, ©1996
(DLC) 87659239
(OCoLC)35784647
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: H F Linskens; J F Jackson; A Bacic
ISBN: 9783642609893 3642609899
OCLC Number: 692580839
Reproduction Notes: Electronic reproduction. [S.l.] : HathiTrust Digital Library, 2010. MiAaHDL
Description: 1 online resource (xvii, 205 pages) : illustrations.
Details: Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.
Contents: Fractionation of Cell Wall Components --
1 Introduction --
2 Polysaccharides --
3 Proteins and Glycoproteins --
4 Lignins --
References --
Isolation and Analysis of Cell Wall Polymers from Olive Pulp --
1 Introduction --
2 Isolation of Cell Walls from Olive Pulp --
3 Sequential Extraction of Cell Wall Polymers --
4 Fractionation of the Extracted Cell Wall Polysaccharides --
5 Chemical and Spectroscopic Analysis --
6 Concluding Remarks --
References --
Determination of Cell Wall Autolysis --
1 Introduction --
2 Factors Altering Cell Wall Isolation and Autolysis --
3 Isolation of Active Cell Walls --
4 Isolation of Inactive Cell Walls (Controls) --
5 Autolysis Incubations --
6 Analysis of Autolysis Products --
7 Conclusions --
References --
Cell Wall Porosity and Its Determination --
1 Introduction --
2 Microscopic Visualization of Wall Pores --
3 Bulk Exclusion Techniques --
4 Tracer Techniques: Uptake of Molecules or Particles --
5 Conclusions --
References --
Analysis of Chitin Biosynthesis --
1 Introduction --
2 Structure of Chitin --
3 The Enzymatic Synthesis of Chitin --
4 Preparation of Chitin Synthase --
5 Product Characterization of Chitin Synthase --
6 Polymerization and Crystallization --
7 Fungal Chitin Synthase Genes --
8 Morphogenetic Roles of Chitin Synthases --
9 Conclusions --
References --
Analysis of Plant-Substratum Adhesives --
1 Introduction --
2 Case Studies of Cell-Substratum Adhesion --
3 Methods for the Analysis of Cell-Substratum Adhesives --
4 Conclusions --
References --
Biochemical, Immunological and Molecular Analyses of Extensin --
1 Introduction --
2 Biochemical Characterization of Extensin --
3 Immunological Detection of Extensin --
4 Molecular Characterization of Extensin --
5 Summary --
References --
Analysis of Pectin Structure by HPAEC-PAD --
1 Introduction --
2 Pectin HPLC Separations --
3 Pectin Analysis in Ripening Peach Fruit --
4 Conclusions and Future Directions --
References --
Characterization of Oligosaccharides Derived from Plant Cell Wall Polysaccharides by On-Line High-Performance Anion-Exchange Chromatography Thermospray Mass Spectrometry --
1 Introduction --
2 Methods --
3 HPAEC in Oligosaccharide Analysis --
4 General Experimental Considerations Related to HPAEC-MS --
5 Application of HPAEC-MS in Oligosaccharide Characterization --
6 Conclusions and Perspectives --
References --
Analysis of Pectin Methyl Esterases --
1 Introduction --
2 Estimation and Detection of PME Activities --
3 PME Localization --
4 PME Extraction and Purification --
5 PME Properties --
6 Roles of PMEs --
7 Conclusions and Perspectives --
References --
Probing the Subunit Composition and Topology of Plasma Membrane-Bound (1,3)-?-Glucan (Callose) Synthases --
1 Introduction --
2 Membrane Isolation, Enzyme Assay, and Solubilization --
3 Callose Synthase Topology, Purification, and Subunit Composition --
4 Biochemical Characterization of Integral Plant Plasma Membrane Proteins --
5 Summary --
References.
Series Title: Modern methods of plant analysis, v. 17.
Responsibility: edited by H.F. Linsksens and J.F. Jackson ; contributors, A. Bacic [and others].

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