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Plant Molecular Biology Manual

Author: Stanton B Gelvin; Robbert A Schilperoort; Desh Pal S Verma
Publisher: Dordrecht : Springer Netherlands, 1989.
Edition/Format:   eBook : Bibliographic data : EnglishView all editions and formats
Database:WorldCat
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Genre/Form: Electronic books
Additional Physical Format: Printed edition:
Material Type: Bibliographic data, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Stanton B Gelvin; Robbert A Schilperoort; Desh Pal S Verma
ISBN: 9789400909519 9400909519 9789401069182 9401069182
OCLC Number: 840304320
Description: 1 online resource (488 pages)
Contents: Section A: Introduction of DNA into Plant Cells --
1. Direct DNA transfer to protoplasts with and without electroporation --
2. Use of cointegrating Ti plasmid vectors --
3. Binary vectors --
4. Agrobacterium molecular genetics --
5. Leaf disc transformation --
6. Extraction of DNA from plant tissues --
7. Procedures for contructing ds-cDNA clone banks --
8. Procedures for constructing genomic clone banks --
9. Selectable and screenable markers --
10. Transformation of plant pathogenic fungi --
Section B: Expression of Genes in Plants --
1. Use of reporter genes to study gene expression in plant cells --
2. Assays for studying chromatin structure --
3. Assays for studying DNA methylation --
4. Analysis of gene expression in transgenic plants --
5. Subcellular targeting of proteins in vivo and in vitro --
6. Isolation of total and polysomal RNA from plant tissues --
7. Translation in Xenopus oocytes of mRNAs transcribed in vitro --
8. Use of the firefly luciferase gene as a reporter of gene expression in plants --
9. In situ RNA hybridization in plant tissues --
10. Light-regulated promoter sequences in the multigene Cab and rbcS gene families --
11. In vitro DNA footprinting --
12. In vivo detection of protein-DNA interactions --
Section C: Fate of Introduced Genes --
1. Stability of introduced genes and stability in expression --
2. Restriction fragment length polymorphism.
Responsibility: edited by Stanton B. Gelvin, Robbert A. Schilperoort, Desh Pal S. Verma.
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