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Plant proteomics : methods and protocols

Author: Hervé Thiellement
Publisher: Totowa, N.J. : Humana Press, ©2007.
Series: Methods in molecular biology (Clifton, N.J.), v. 355.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Database:WorldCat
Summary:
The aim of Plant Proteomics: Methods and Protocols is to present up-- date methods and protocols used by recognized scientists in the world of plant proteomics. If this world was a very small one twenty-five years ago when the first papers were published, it has since experienced exponential growth, and in most countries around the world there are laboratories working on plant proteomics. Two-dimensional gel  Read more...
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Genre/Form: Laboratory Manuals
Electronic books
Additional Physical Format: Print version:
Plant proteomics.
Totowa, N.J. : Humana Press, ©2007
(DLC) 2006041207
(OCoLC)64453506
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Hervé Thiellement
ISBN: 1588296350 9781588296351 1597452270 9781597452274 1280832681 9781280832680
OCLC Number: 184907752
Description: 1 online resource (xiii, 399 pages) : illustrations.
Contents: Total protein extraction with TCA-acetone / Valérie Méchin, Catherine Damerval, and Michel Zivy --
Phenol extraction of proteins for proteomic studies of recalcitrant plant tissues / Mireille Faurobert, Esther Pelpoir, and Jamila Chaïb --
Protein extraction from cereal seeds / Gérard Branlard and Emmanuelle Blancel --
Protein extraction from xylem and phloem sap / Julia Kehr and Martijn Rep --
Protein extraction from woody plants / Christophe Plomion and Céline Lalanne --
Isolation of chloroplast proteins from Arabidopsis thaliana for proteome analysis / Klaas J. van Wijk, Jean-Benoit Peltier, and Lisa Giacomelli --
Isolation and subfractionation of plant mitochondria for proteomic analysis / Holger Eubel, Joshua L. Heazlewood, and A. Harvey Millar --
Extraction of nuclear proteins from root meristematic cells / Fernando González-Camacho and Francisco Javier Medina --
Isolation of nuclear proteins / Setsuko Komatsu --
Isolation of cell wall proteins from Medicago sativa stems / Bonnie S. Watson and Lloyd W. Sumner --
Plant plasma membrane protein extraction and solubilization for proteomic analysis / Véronique Santoni --
Detergents and chaotropes for protein solubilization before two-dimensional electrophoresis / Thierry Rabilloud [and others] --
Two-dimensional electrophoresis for plant proteomics / Walter Weiss and Angelika Görg --
Visible and fluorescent staining of two-dimensional gels / François Chevalier, Valérie Rofidal, and Michel Rossignol --
Two-dimensional differential in-gel electrophoresis (DIGE) of leaf and roots of Lycopersicon esculentum / Matthew Keeler [and others] --
Quantitative analysis of 2D gels / Michel Zivy --
Multivariate data analysis of proteome data / Kåre Engkilde, Susanne Jacobsen, and Ib Søndergaard --
Edman sequencing of plant proteins from 2D gels / Setsuko Komatsu --
Peptide mass fingerprinting: identification of proteins by MALDI-TOF / Nicolas Sommerer, Delphine Centeno, and Michel Rossignol --
Protein identification using nano liquid chromatography-tandem mass spectrometry / Luc Negroni --
Two-dimensional nanoflow liquid chromatography-tandem mass spectrometry of proteins extracted from rice leaves and roots / Linda Breci and Paul A. Haynes --
Separation, identification, and profiling of membrane proteins by GFC/IEC/SDS-PAGE and MALDI TOF MS / Wojciech Szponarski [and others] --
The PROTICdb database for 2-DE proteomics / Olivier Langella, Michel Zivy, and Johann Joets --
Identification of phosphorylated proteins / Maria V. Turkina and Alexander V. Vener --
Plant proteomics and glycosylation / Anne-Catherine Fitchette [and others] --
Blue-native gel electrophoresis for the characterization of protein complexes in plants / Jesco Heinemeyer, Dagmar Lewejohann, and Hans-Peter Braun --
Electroelution of intact proteins from SDS-PAGE gels and their subsequent MALDI-TOF MS analysis / Zhentian Lei [and others] --
Generation of plant protein microarrays and investigation of antigen-antibody interactions / Birgit Kersten and Tanja Feilner --
Phosphorylation studies using plant protein microarrays / Tanja Feilner and Birgit Kersten.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 355.
Responsibility: edited by Hervé Thiellement [and others].
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Abstract:

Aims to provide methods and protocols used by recognized scientists in the world of plant proteomics. This work explains their experimental methodology, either in the wet or in the dry lab, enabling  Read more...

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