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Plant transcription factors : methods and protocols

Author: Ling Yuan; Sharyn E Perry
Publisher: New York : Humana Press, ©2011.
Series: Methods in molecular biology (Clifton, N.J.), v. 754.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
Recent years have seen significant advancements in the development of enabling technologies that facilitate the study of Transcription Factors (TFs). TFs are pivotal in the regulation of plant development, reproduction, intercellular signaling, response to environment, cell cycle, and metabolism. Plant Transcription Factors: Methods and Protocols offers a comprehensive approach by covering the basic concepts as well  Read more...
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Genre/Form: Electronic books
Additional Physical Format: Print version:
Plant transcription factors.
New York : Humana, 2011
(DLC) 2011931514
(OCoLC)723107867
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Ling Yuan; Sharyn E Perry
ISBN: 9781617791543 1617791547
OCLC Number: 740821635
Language Note: English.
Description: 1 online resource (xi, 347 pages) : illustrations.
Contents: MADS and more: Transcription factors that shape the plant --
In silico mining and PCR-based approaches to transcription factor discovery in non-model plants: Gene discovery of the WRKY transcription factors in conifers --
Isolation of plant transcription factors using a yeast one-hybrid system --
A transposon-based activation tagging system for gene function discovery in arabidopsis --
CRES-T, an effective gene silencing system utilizing chimeric repressors --
Analysis of a transcription factor using transient assay in arabidopsis protoplasts --
Microarray-based identification of transcription factor target genes --
Yeast protein-protein interaction assays and screens --
Mapping functional domains of transcription factors --
Bimolecular fluorescence complementation as a tool to study interactions of regulatory proteins in plant protoplasts --
The hepatitis nucleocapsid as a vaccine carrier moiety --
Isolation of transcription factor complexes from arabidopsis cell suspension cultures by tandem affinity purification --
Assaying transcription factor stability --
How to assess the intercellular trafficking of transcription factors --
SELEX (systematic evolution of ligands by exponential enrichment), as a powerful tool for deciphering the protein-DNA interaction space --
Footprinting and missing nucleoside analysis of transcription factor-DNA complexes --
Chromatin immunoprecipitation to verify or to identify in vivo protein-DNA interactions --
Visualizing and characterizing in vivo DNA-binding events and direct target genes of plant transcription factors --
Mapping in vivo protein-DNA interactions in plants by damid, a DNA adenine methylation-based method --
Directed evolution through DNA shuffling for the improvement and understanding of genes and promoters --
Thiophilic interaction chromatography of prostate-specific antigen.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 754.
Responsibility: edited by Ling Yuan, Sharyn E. Perry.

Abstract:

Recent years have seen significant advances in technologies that enable us to analyze transcription factors, a pivotal cell regulatory process in plants. This comprehensive survey covers the basic  Read more...

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From the reviews:"It represents a collection of protocols for performing analysis on plant transcription factors (TFs). ... this book will be very useful to many research groups. The different Read more...

 
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