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Plants from test tubes : an introduction to micropropagation

Verfasser/in: Lydiane Kyte; John G Kleyn
Verlag: Portland, Or. : Timber Press, ©1996.
Ausgabe/Format   Print book : Englisch : 3rd edAlle Ausgaben und Formate anzeigen
Datenbank:WorldCat
Zusammenfassung:
Originally published in 1983, Plants from Test Tubes was one of the first how-to books on plant tissue culture or "cloning." It continues to be an internationally popular primer. New to this third edition is in-depth information on culture contaminants, a tutorial on the use of the microscope, and a discussion of the role of tissue culture as an indispensable tool in modern biotechnology. Plants from Test Tubes
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Gattung/Form: Laboratory manuals
Manuels de laboratoire
Dokumenttyp: Buch
Alle Autoren: Lydiane Kyte; John G Kleyn
ISBN: 0881923613 9780881923612
OCLC-Nummer: 34321678
Beschreibung: 240 pages : illustrations ; 27 cm
Inhalt: Foreword / Michael E. Kane --
Sect. I. The Basics of Tissue Culture. Ch. 1. Overview. Ch. 2. The Botanical Basis for Tissue Culture. Ch. 3. History. Ch. 4. The Laboratory --
Facilities and Supplies. Ch. 5. Media Ingredients. Ch. 6. Media Preparation. Ch. 7. Explants and Their Preparation. Ch. 8. The Transfer Room. Ch. 9. The Growing Room. Ch. 10. Hardening Off. Ch. 11. Dealing with Biological Contaminants. Ch. 12. Business. Ch. 13. Biotechnology --
Sect. II. Culture Guide to Selected Plants. Ferns. Conifers. Monocotyledons. Dicotyledons --
App. A. Metric Conversions --
App. B. The Microscope --
Introduction, Use, and Care --
App. C. Professional Organizations --
App. D. Suppliers.
Verfasserangabe: Lydiane Kyte and John Kleyn.
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Abstract:

Originally published in 1983, Plants from Test Tubes was one of the first how-to books on plant tissue culture or "cloning." It continues to be an internationally popular primer. New to this third edition is in-depth information on culture contaminants, a tutorial on the use of the microscope, and a discussion of the role of tissue culture as an indispensable tool in modern biotechnology. Plants from Test Tubes remains the most accessible and practical book on the subject. The book is divided into two sections. The first, "The Basics of Tissue Culture," begins with a discussion of the historical and botanical background of micropropagation. A short course in chemistry lays the foundation for media preparation instructions. Sterile technique and culture care are described in detail. Advice is offered on how to deal with possible problems with cultures, including such topics as vitrification, lack of growth, and contamination.

Section II, "Culture Guide to Selected Plants," provides recipes for propagating 54 varieties of ferns, conifers, and flowering plants - an increase of 15 percent from the previous edition. An extensive bibliography and a large appendix of suppliers of chemicals and equipment complete this comprehensive edition.

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