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Plasmids : a practical approach

Author: K G Hardy
Publisher: Oxford ; New York : IRL Press at Oxford University Press, ©1993.
Series: Practical approach series, 138.
Edition/Format:   Print book : English : 2nd edView all editions and formats
Summary:

Provides research workers with protocols for studying bacterial plasmids, and for using both plasmids and phagemids as vectors. Techniques for studying fundamental aspects of plasmids, such as  Read more...

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Additional Physical Format: Online version:
Plasmids.
Oxford ; New York : IRL Press at Oxford University Press, ©1993
(OCoLC)608167583
Online version:
Plasmids.
Oxford ; New York : IRL Press at Oxford University Press, ©1993
(OCoLC)622280240
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: K G Hardy
ISBN: 0199634459 9780199634453 0199634440 9780199634446
OCLC Number: 28293338
Description: xviii, 252 pages : illustrations ; 24 cm.
Contents: Plasmid replication and maintenance : Introduction --
Single-cell resistance (SCR) to antibodiotics --
Determination of plasmid copy numbers --
Partition and other maintenance functions --
Incompatibility --
Basic replicons --
Origins (ori) of replication --
Rep functions --
Cop functions --
Replicative intermediates --
Timing of replication --
In vitro replication --
Visualization of plasmids / Kurt Nordström --
Plasmids from gram-positive bacteria : Introduction --
Classification --
Isolation of plasmid DNA --
Host range and plasmid transfer --
Plasmid replication modes / Juan C. Alonso and Manuel Espinosa --
Lactococcal plasmid vectors : Introduction --
Lactococcal vector systems / K.J. Leenhouts and G. Venema. Virulence plasmids : Introduction --
Isolation of plasmids --
Curing of plasmids --
Mutagenesis --
Fast and small electrophoresis sequencing gels--
Comparison of helical stability of DNA fragments --
Identification of plsamid-encoded products --
RNA extraction and analysis / Marcelo E. Tolmasky, Luis A. Actis, and Jorge H. Crosa --
Plasmids in Agrobacterium tumefaciens and Rhodococcus fascians : Introduction --
Identification of endogenous plasmids by small scale isolation procedures --
Large scale purification methods --
Introduction of recombinant plasmids --
Analysis of plasmid-encoded functions / Jan Desomer, Rolf Deblaere, and Marc Van Montagu --
Streptomyces plasmid vectors : Introduction --
Autonomously replicating Streptomyces vectors --
Integrating vectors --
Gene disruption/replacement techniques using temperature-sensitive and non-replicative plasmids / W. Wohlleben and G. Muth. Use of [Lambda]-plasmid composite vectors for expression cDNA cloning : Introduction --
Expression in cloning using unidrectional cDNA libraries --
Generation of cDNA libraries by the ADC method --
Analysis of cloned cDNAs --
Applications / Toru Miki and Stuart A. Aaronson --
Phagemids and other hybrid vectors : Introduction --
Early Vectors --
New applications --
Phagemids and lambda ZAP cloning --
Future / Michelle A. Alting-Mees, Peter Vaillancourt, and Jay M. Short --
Extrachromosomal cloning vectors of Saccharomyces cerevisiae : Introduction --
Basic cloning vectors of S. cerevisiae --
Multifunctional cloning vectors of S. cerevisiae --
Yeast host strain --
Introduction of DNA into S. cerevisiae / Robert S. Sikorski.
Series Title: Practical approach series, 138.
Responsibility: edited by K.G. Hardy.
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The book will be essential reading for all those using recombinant DNA techniques. * Aslib Book Guide, Vol. 59, No. 4, April 1994 * 'this volume provides practical guidance on plasmid replication and Read more...

 
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