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Potassium channels : methods and protocols

Author: Jonathan D Lippiat
Publisher: New York, NY : Humana Press, ©2008.
Series: Springer protocols (Series); Methods in molecular biology (Clifton, N.J.), v. 491.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
Due to the importance of potassium channels in cellular function, researchers have been challenged to develop tools and experimental procedures to probe the function and identify chemicals that modulate the behaviour of this super-family of ion channels. In Potassium Channels: Methods and Protocols, experts in the field present a range of experimental approaches that have been developed to investigate potassium  Read more...
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Additional Physical Format: Print version:
Potassium channels.
New York, NY : Humana Press, ©2008
(DLC) 2008931178
(OCoLC)236120873
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Jonathan D Lippiat
ISBN: 9781597455268 1597455261
OCLC Number: 300493429
Language Note: English.
Description: 1 online resource (x, 300 pages) : illustrations.
Contents: Identifying transcriptional regulatory regions using reporter genes and DNA-protein interactions by chromatin immunoprecipitation / Lezanne Ooi and Ian C. Wood --
Quantitative RT-PCR methods for investigation of low copy potassium channel gene expresssion in native murine arteries / Alex Cheong, Samuel J. Fountain, and David J. Beech --
Cloning of potassium chanel splice variants from tissues and cells / Lie Chen and Michael J. Shipston --
Chemiluminescence assays to investigate membrane expression and clathrin-mediated endocytosis of KATP channels / Andrew J. Smith and Asipu Sivaprasadarao --
Investigation of KATP channel endocytosis by immunofluorescence / Andrew J. Smith and Asipu Sivaprasadarao --
Investigation of KATP channel endocytosis and cell surface density by biotinylation and western blotting / Andrew J. Smith and Asipu Sivaprasadarao --
Lipid microdomains and K+ channel compartmentation : detergent and non-detergent-based methods for the isolation and characterisation of cholesterol-enriched lipid rafts / Laura J. Sampson and Caroline Dart --
Determination of phosphoinositide binding to K+ channel subunits using a protein-lipid overlay assay / Alison M. Thomas and Andrew Tinker --
Protein complex analysis of native brain potassium channels by proteomics / Guillaume Sandoz and Florian Lesage --
Xenopus oocytes as a heterologous expression system for studying ion channels with the patch-clamp technique / Paolo Tammaro, Kenju Shimomura, and Peter Proks --
Whole-cell recording using the perforated patch clamp technique / Jonathan D. Lippiat --
Recording the activity of ATP-sensitive K+ channels in open-cell cell-attached configuration / Andrei I. Tarasov --
Planar patch clamp : advances in electrophysiology / Andrea Brüggemann [and others] --
Analysing steroid modulation of BKCa channels reconstituted into planar lipid bilayers / Heidi de Wet, Jonathan D. Lippiat, and Marcus Allen --
Using bioluminescence resonance energy transfer to measure ion channel assembly / Gina M. Whitaker and Eric A. Accili --
The use of FRET microscopy to elucidate steady state channel conformational rearrangements and G protein interaction with the GIRK channels / Adi Raveh, Inbal Riven, and Eitan Reuveny --
The voltage-clamp fluorometry technique / Chris S. Gandhi and Riccardo Olcese --
Identification of mutations in the Kir6.2 subunit of the KATP channel / Sarah E. Flanagan and Sian Ellard --
Modulation of potassium ion channel proteins utilising antibodies / Mark L. Dallas, Susan A. Deuchars, and Jim Deuchars --
Fluorescence-based Tl+-influx assays as a novel approach for characterization of small-conductance CA²-activated K+ channel modulators / Susanne Jørgensen, Tina H. Johansen, and Tino Dyhring --
Rubidium efflux as a tool for the pharmacological characterisation of compounds with BK channel opening properties / Neil G. McKay, Robert W. Kirby, and Kim Lawson --
Recording hERG potassium currents and assessing the effects of compounds using the whole-cell patch-clamp technique / Ray M. Helliwell.
Series Title: Springer protocols (Series); Methods in molecular biology (Clifton, N.J.), v. 491.
Responsibility: edited by Jonathan D. Lippiat.

Abstract:

In Potassium Channels: Methods and Protocols, experts present a range of experimental approaches. These have been developed to investigate potassium channel structure, function, pharmacology, cell  Read more...

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From the reviews:"This is a very informative book, covering a variety of methods available to study potassium channels ... . The protocols are clearly organized and usefully detailed. ... Read more...

 
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