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Practical capillary electrophoresis

Author: Robert Weinberger
Publisher: Boston : Academic Press, ©1993.
Edition/Format:   Print book : EnglishView all editions and formats
Database:WorldCat
Summary:

High performance capillary electrophoresis (HPCE) is perhaps the most powerful separation technique available. This text aims to take the "black art" out of HPCE by providing a comprehensive look at  Read more...

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Additional Physical Format: Online version:
Weinberger, Robert.
Practical capillary electrophoresis.
Boston : Academic Press, ©1993
(OCoLC)623502274
Online version:
Weinberger, Robert.
Practical capillary electrophoresis.
Boston : Academic Press, ©1993
(OCoLC)632101832
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: Robert Weinberger
ISBN: 0127423559 9780127423555
OCLC Number: 27222974
Description: xv, 312 pages : illustrations ; 24 cm
Contents: Introduction --
Electrophoresis --
Microchromatographic separation methods --
Capillary electrophoresis --
Historical perspective --
Instrumentation --
Sources of information on HPCE --
Capillary electrophoresis: a family ot techniques --
Basic concepts --
Electrical conduction in fluid solution --
Language of electrophoresis --
Electroendoosmosis --
Efficiency --
Resolution --
Joule heating --
Optimizing the voltage and temperature --
Capillary diameter and buffer ionic strength --
Optimizing the capillary length --
Buffers --
Capillaries --
Capillary zone electrophoresis --
Mobility --
Buffer additives --
Solute-wall interactions --
Bandbroadening --
Applications --
Methods development --
Capillary isoelectric focusing --
Capillary coatings and electroosmotic flow --
Performing a run --
Focusing and mobilization problems --
Applications --
Size separations in capillary gels and polymer networks --
Separation mechanism --
Materials for CGE --
Applications --
Pulsed-field capillary electrophoresis --
Future of CGE --
Capillary isotachophoresis --
Separation mechanism --
Instrumental aspects --
Buffer selection. (cont) Advantages and problems with CITP --
Applications --
Electrokinetic capillary chromatography --
Micelles --
Separation mechanism --
Sources of bandbroadening --
Selecting the electrolyte system --
Elution range of MECC --
Alternative surfactant systems --
Applications and methods development --
Cyclodextrins --
Chiral recognition --
Optimization --
Capillary electrochromatography --
Electroosmotic pump --
Bandbroadening --
Applications --
Electrochromatography in perspective --
Injection --
Volumetric constraints on injection size --
Performing an injection and run --
Injection techniques --
Injection problems and solutions --
Alternative injection schemes --
Stacking --
Other enrichment techniques --
Detection --
On-capillary detection --
Detection problem --
Classes of detectors --
Limits of detection --
Bandbroadening --
Absorption detection --
Fluorescence detection --
Indirect detection --
Mass spectrometry --
Other detection techniques --
Fraction collection --
Putting it all together --
Selecting the mode of HPCE --
Requirements for robust separations. (cont) Realistic compromises --
Linear dynamic range --
Sample preparation --
Quantitative analysis --
Correction factors --
Special topics --
Field effect electroendoosmosis --
Ultrafast separations --
HPCE on a chip --
Multiple capillary systems --
Gradient and programmed systems --
Two-dimensional systems --
Immunologic trace enrichment.
Responsibility: Robert Weinberger.

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