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Protein arginylation : methods and protocols

Author: A Kashina
Publisher: New York, NY : Humana Press, [2015] ©2015
Series: Methods in molecular biology (Clifton, N.J.), volume 1337.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Database:WorldCat
Summary:
"This volume presents a comprehensive overview of all the existing methods on analysing protein arginylation, from the early methods utilizing crude protein preparations and whole-cell assays to the latest advanced methods involving recombinant protein techniques, antibodies, high precision mass spectrometry, and chemical probes. This book also includes essays from the founders of the field, who originally  Read more...
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Genre/Form: Electronic books
Laboratory manuals
Laboratory Manuals
Additional Physical Format: Print version:
Protein Arginylation.
Humana Press Inc 2015
(OCoLC)908374364
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: A Kashina
ISBN: 9781493929351 1493929356 1493929348 9781493929344
OCLC Number: 919313248
Description: 1 online resource (ix, 149 pages) : illustrations (some color).
Contents: Protein arginylation: Over 50 years of discovery --
Recollection of how we came across the protein modification with amino acids by aminoacyl trna-protein transferase --
Arginyltransferase: A personal and historical perspective --
Arginylation in a partially purified fraction of 150k x g supernatants of axoplasm and injured vertebrate nerves --
Preparation of ate1 enzyme from native mammalian tissues --
Correlated measurement of endogenous ate1 activity on native acceptor proteins in tissues and cultured cells to detect cellular aging --
Assaying the posttranslational arginylation of proteins in cultured cells --
Assaying ate1 activity in yeast by beta-gal degradation --
Bacterial expression and purification of recombinant arginyltransferase (ate1) and arg-trna synthetase (rrs) for arginylation assays --
Assaying ate1 activity in vitro --
High-throughput arginylation assay in microplate format --
Assay of arginyltransferase activity by a fluorescent hplc method --
Identification of arginylated proteins by mass spectrometry --
Analysis of arginylated peptides by subtractive edman degradation --
Transferase-mediated labeling of protein n-termini with click chemistry handles --
Applying arginylation for bottom-up proteomics --
Development of new tools for the studies of protein arginylation.
Series Title: Methods in molecular biology (Clifton, N.J.), volume 1337.
Responsibility: edited by Anna S. Kashina.
More information:

Abstract:

"This volume presents a comprehensive overview of all the existing methods on analysing protein arginylation, from the early methods utilizing crude protein preparations and whole-cell assays to the latest advanced methods involving recombinant protein techniques, antibodies, high precision mass spectrometry, and chemical probes. This book also includes essays from the founders of the field, who originally discovered arginylation in the early 1960s and brought it to international recognition. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Protein Arginylation: Methods and Protocols would interest the emerging body of scientists involved in the studies of posttranslational arginylation and the rapidly growing community of researchers working on a broad range of posttranslational modifications, the analysis of which often meets similar challenges and utilizes similar principles as posttranslational arginylation."--

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