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Protein expression in mammalian cells : methods and protocols

Author: James Hartley
Publisher: New York : Humana Press, ©2012.
Series: Springer protocols (Series); Methods in molecular biology (Clifton, N.J.), v. 801.
Edition/Format:   Print book : EnglishView all editions and formats
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Protein Expression in Mammalian Cells presents a compendium of vital techniques to further our knowledge of mammalian protein expression. Each chapter includes a brief introduction, step-by-step,  Read more...

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Genre/Form: Laboratory manuals
Laboratory Manuals
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: James Hartley
ISBN: 9781617793516 1617793515 9781617793523 1617793523
OCLC Number: 733249844
Description: xi, 276 pages : illustrations ; 26 cm.
Contents: Why proteins in mammalian cells? / James L. Hartley --
Large-scale transfection of mammalian cells / Lucia Baldi [and others] --
Selection of high expressing mammalian cells by surface display of reporters / Christine T. DeMaria --
Expression of secreted protein in mammalian cells using baculovirus particles / Barbara Ann Jardin, Cynthia B. Elias, and Satya Prakash --
Transfection of difficult-to-transfect primary mammalian cells / Oliver Gresch and Ludger Altrogge --
Stable protein expression in mammalian cells using baculoviruses / Andreas Lackner [and others] --
Using matrix attachment regions to improve recombinant protein production / Niamh Harraghy [and others] --
Controlling apoptosis to optimize yields of prtoeins from mammalian cells / Matthew P. Zustiak [and others] --
Post-transcriptional regulatory elements for enhancing transient gene expression levels in mammalian cells / Mariati [and others] --
Converting monoclonal antibodies into Fab fragments for transient expression in mammalian cells / Joanne E. Nettleship [and others] --
Generation of high-expressing cells by methotrexate amplification of destabilized dihydrofolate reductase selection marker / Say Kong Ng --
Tools for coproducing multiple proteins in mammalian cells / Zahra Assur, Wayne A. Hendrickson, and Filippo Mancia --
Identification and characterization of protein glycosylation using specific endo- and exoglycosidases / Paula Magnelli, Alicia Bielik, and Ellen Guthrie --
Strategies for efficient transfection of CHO-cells with plasmid DNA / Renate Kunert and Karola Vorauer-Uhl --
Methods for constructing clones for protein expression in mammalian cells / Takefumi Sone and Fumio Imamoto --
Optimizing transient recombinant protein expression in mammalian cells / Ralph F. Hopkins, Vanessa E. Wall, and Dominic Esposito.
Series Title: Springer protocols (Series); Methods in molecular biology (Clifton, N.J.), v. 801.
Responsibility: edited by James L. Hartley.
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From the reviews:"This book is really a great help for all those that wish to enter the field and I am sure even for those who are already involved with recombinant protein production in mammalian Read more...

 
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