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Protein NMR techniques

Author: A Kristina Downing
Publisher: Totowa, N.J. : Humana Press, ©2004.
Series: Methods in molecular biology (Clifton, N.J.), v. 278.
Edition/Format:   eBook : Document : English : 2nd edView all editions and formats
Summary:
Carrying on in the tradition of its much praised earlier edition, A. Kristina Downing has selected new protocols describing the many recent advances in NMR methodology that are revolutionizing the field. Described in step-by-step detail by established hands-on experts, these readily reproducible techniques include methods for high-level recombinant protein expression using sophisticated isotopic labeling strategies,  Read more...
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Genre/Form: Electronic books
Additional Physical Format: Print version:
Protein NMR techniques.
Totowa, N.J. : Humana Press, ©2004
(DLC) 2003027535
(OCoLC)53955192
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: A Kristina Downing
ISBN: 1588292460 9781588292469 1592598099 9781592598090
OCLC Number: 61264161
Language Note: English.
Description: 1 online resource (xi, 487 pages) : illustrations.
Contents: Screening and optimizing protein production in e. coli / Lorraine Hewitt and James M. McDonnell --
Isotopic labelling of recombinant proteins from the methylotrophic yeast pichia pastoris / Andrew R. Pickford and Joanne M. O'Leary --
Perdeuteration/site-specific protonation approaches for high mw --
Proteins / Stephen Matthews --
Segmental isotopic labelling for structural biological applications of NMR / David Cowburn [and others] --
TROSY-based correlation and NOE spectroscopy for NMR structural studies of large proteins / Guang Zhu [and others]. Media for studies of partially aligned states / Kieran Fleming and Stephen Matthews --
Residual dipolar couplings in protein structure determination / Eva de Alba and Nico Tjandra --
Projection angle restraints for studying structure and dynamics of biomolecules / Christian Griesinger [and others] --
Characterizing domain interfaces by NMR / Luke M. Rooney, Sachchidanand, and Jörn M. Werner --
Characterization of the overall rotational diffusion of a protein from 15N relaxation measurements and hydrodynamic calculations / Jennifer Blake Hall, Oliver Walker, and David Fushman. TROSY-based NMR experiments for the study of macromolecular dynamics and hydrogen bonding / Guang Zhu [and others] --
Measurement of intermediate exchange phenomena / James G. Kempf and J. Patrick Loria --
NMR studies of partially folded molten globule states / Christina Redfield --
Structure determination of protein complexes by NMR / Daniel Nietlispach [and others] --
NMR studies of protein/nucleic acid interactions / Gabriele Varani, Yu Chen, and Thomas C. Leeper --
Using NMRview to visualize and analyze the NMR spectra of macromolecules / Bruce A. Johnson. Automated NMR structure calculation with CYANA / Peter Güntert --
NOE assignment with ARIA 2.0: the nuts and bolts / Michael Habeck [and others] --
Membrane protein structure determinations using solid state NMR / Anthony Watts [and others].
Series Title: Methods in molecular biology (Clifton, N.J.), v. 278.
Responsibility: edited by A. Kristina Downing.
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Abstract:

Staying with the idea of isotopic labeling, Chapter 3 describes methods for perdeuteration and site-specific protonation and is the first of several chapters in the book that is relevant to studies  Read more...

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From reviews of the first edition..."...includes many good practical details which are so often omitted and which workers new to the field have to rediscover..." -Analyst "...an Read more...

 
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