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The protein protocols handbook

Author: John M Walker
Publisher: New York, N.Y. : Humana Press, ©2009.
Edition/Format:   Print book : English : 3rd edView all editions and formats

The third edition of The Protein Protocols Handbook introduces 57 critically important new chapters, and significantly updates the previous edition's tried-and-trusted methods. The book offers over  Read more...


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Genre/Form: Handbooks and manuals
Handbooks, manuals, etc
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: John M Walker
ISBN: 9781603274746 160327474X
OCLC Number: 505003419
Description: xxxv, 1991 pages : illustrations ; 26 cm
Contents: Part I : QUANTITATION OF PROTEINS 1. Protein Determination by UV Absorption A. Aitken and M.P. Learmonth2. The Lowry Method for Protein Quantitation J. H. Waterborg 3 The Bicinchoninic Acid (BCA) Assay for Protein Quantitation J.M. Walker4 The Bradford Method for Protein Quantitation N.Kruger 5. Ultrafast Protein Determinations Using Microwave Enhancement R. Akins and R. S. Tuan 6. The Nitric Acid Methods for Protein Estimation in Biological Samples S.A. Boerner, Y.K. Lee, S.H. Kaufmann and K. Bible7. Quantitation of Tryptophan in Proteins A. Aitken and M.P. Learmonth8. Kinetic Silver Staining of Proteins D. D. Root and K. Wang 9. Quantitation of cellular proteins by flow cytometry T. D. Friedrich , F.A. Ray, R.L. Smith and J. M. Lehman10. Quantitation of cellular proteins by laser scanning cytometryT.D. Friedrich, R.L.Smith and J.M. Lehman Part II : ELECTROPHORESIS OF PROTEINS AND PEPTIDES AND DETECTION IN GELS 11 Protein Solubility in 2D Electrophoresis: Basic principles and issues T.Rabilloud12 Mouse and Human Tissues Sample Preparation for 2-D Electrophoresis C. Zabel and J. Klose13 Plant Protein sample preparation for 2DE S. Carpentier , R.Swennen and B. Panis 14 Preparation of bacterial samples for 2-D PAGE. B. Vandahl, G. Christiansen and S. Birkelund15 Preparation of bodily fluids for 2-D PAGE Sullivan, H.Brzeski, J. Ganesalingam, and M. Mayr16 Immunoaffinity Depletion of high abundance plasma and serum proteinsL.A. Echan and D. Speicher 17 Preparation of Yeast samples for 2D PAGE J. Norbeck18 Membrane Protein Preparation Using Aqueous Polymer Two Phase Systems J. Schindler and H-G Nothwang19 Subcellular fractionation of small sample amounts H.G Nothwang, I. Guillemin and J. Schindler20. Nondenaturing Polyacrylamide Gel Electrophoresis of Proteins J.M. Walker21 SDS Polyacrylamide Gel Electrophoresis of Proteins J.M. Walker22 Gradient SDS Polyacrylamide Gel Electrophoresis of Proteins J.M. Walker23. SDS-Polyacrylamide Gel Electrophoresis of Peptides R. C. Judd24 Blue native gel electrophoresis (BN-PAGE) Elke A. Dian, J. Rassow and Christian Motz 25 Separation of proteins by gel electrophoresis in the Tris-Taurine-HCl system S.Luche, M. Chevallet C. Lelong and T. Rabilloud 26 Cetyltrimethylammonium Bromide Discontinuous Gel Electrophoresis of Proteins: M -Based Separation of Proteins with Retained Native ActivityR.E. Akins and R. S. Tuan 27 Acetic Acid-Urea Polyacrylamide Gel Electrophoresis of Basic Proteins J. H. Waterborg28 Acid-Urea-Triton Polyacrylamide Gel Electrophoresis of Histones J. H. Waterborg 29 Isoelectric Focusing of Proteins in Ultra-Thin Polyacrylamide Gels J. M. Walker30 Serial immobilized pH gradient isoelectric focusing over pH 4-9 S.Poznanovic, W. Wozny, H. Zengerling, G.P. Schwall and M. A. Cahill 31. Radiolabelling of Eukaryotic Cells and Subsequent Preparation for 2-D <N. Bizios32 Two-Dimensional PAGE Using Carrier Ampholyte pH Gradients in the First Dimension P.Gravel33 Vertical agarose electrophoresis and electroblotting of high molecular weight proteins M. Greaser and C.M. Warren34 2D PAGE of high molecular weight proteins M. Oh-Ishi and T.Maeda 35 Casting immobilised pH gradients E. Gianazza 36 Nonequilibrium pH Gel Electrophoresis J. Carroll 37 Microchip capillary electrophoresis B.A. Fogarty, N.A.Lacher and S.M. Lunte38 Protein Separations in Microfluidic Chips A. Chow and B.Fathollahi 39 Difference gel electrophoresis (DIGE) D. Friedman and K.S. Lilley 40 Comparing 2-D Electrophoresis Gels Across Internet Databases P. Lemkin, G.C.Thornwall, and J.A. Evans 41 Quantification of Radiolabeled Proteins in Polyacrylamide Gels 25.4.07 W.R. Springer 42 Differential ProteoTope radioactive quantification of protein abundance ratiosW.Wozny, G.P.Schwall, C.S. Sastri, S.Poznanovic, W. Stegmann, Hunzinger, K. Groebe and M.A. Cahill43 Quantitation of Proteins on Polyacrylamide Gels J. Smith44 Using SDS-PAGE and scanning laser densitometry to measure proteinsAaron P. Miles and A. Saul 45 Rapid and Sensitive Staining of Unfixed Proteins in Polyacrylamide Gels with Nile Red J-R. Daban, S. B.A. Bermudez amd F.J. Alba 46 Zinc reverse staining technique C. Fernandez-Patron 47 Protein Staining with Calconcarboxylic Acid in Polyacrylamide Gels Wei-Tao Cong, Sun-Young Hwang, Li-Tai Jin and Jung-Kap Choi48 Detection of Proteins in Polyacrylamide Gels by Silver Staining M. J. Dunn 49 Background-free Protein Detection on Polyacrylamide Gels and on Electroblots Using Transition Metal Chelate Stains W.F. Patton50 Detection of Proteins in Polyacrylamide Gels by Fluorescent Staining M. J. Dunn51 Detection of Glycoproteins in Gels and Blots Nicolle Packer, Malcolm S Ball, Peter L Devine, and Wayne F Patton52 Staining of Glycoproteins/Proteoglycans on SDS-Gels H.J. Moller and J.H. Poulsen 53. Detection of Proteins and Sialoglycoproteins in Polyacrylamide Gels Using Eosin X stain F. Lin & G. Wise54 Pro-Q Diamond phosphoprotein staining G.K. Agrawal and J.J. Thelen55. Electroelution of Proteins from Polyacrylamide Gels P. Jenoe and Martin Horst56 Autoradiography and Fluorography of Acrylamide Gels A. Circolo and S. Gulati57 Proteolytic Activity Detection by Two-Dimensional Zymography J.Wilkesman Part III : BLOTTING AND DETECTION METHODS 58. Protein Blotting by Electroblotting M. Page and R.Thorpe59. Protein Blotting by the Semi-dry Method P. Gravel 60. Protein Blotting by the Capillary Method J.M. Walker61. Western Blotting of basic proteins electrophoretically resolved on acid-urea-Triton-polyacrylamide gels G.P. Delcuve and J. R. Davie62 Immunoblotting of 2-DE Separated Proteins Magi and L Bianchi 63 High efficiency blotting of high-molecular weight proteins M. Greaser and D.R. Swartz64. Alkaline Phosphatase Labeling of IgG Antibody G. B.Wisdom 65. b-Galactosidase Labeling of IgG Antibody G. B. Wisdom 66. Horseradish Peroxidase Labeling of IgG Antibody G. B. Wisdom 67. Digoxigenin (DIG) Labelling of IgG Antibody G. B. Wisdom 68. Conjugation of fluorochromes to antibodies S-Y. Mao 69. Coupling of Antibodies with Biotin R.P. Haugland and Wendy W. You70. Preparation of Avidin Conjugates R.P. Haugland and M.K. Bhalgat 71. MDPF Staining of Proteins on Western Blots F. Alba and J.R. Daban 72. Copper Iodided Staining of Proteins and its silver enhancement D. D. Root and K. Wang73. Detection of Proteins on Blots using Direct Blue 71 Wei-Tao Cong, Sun-Young Hwang, Li-Tai Jin and Jung-Kap Choi74 Detection of proteins on Western blots using colorimetric and radiometric vusialization of Secondary Ligands N. J. Kruger 75. Identification of Glycoproteins on Nitrocellulose Membranes Using Lectin BlottingP. Gravel 76. A Sensitive Method to Quantitatively Detect Total Protein on Membranes after Electrophoretic Transfer Using Avidin- or Streptavidin-Biotin W.J. LaRochelle 77. Detection and Quantification of Proteins on Immunoblots using Enhanced Chemiluminescence J. Young 78 Reutilization of Western Blots After Chemiluminescent Detection or Autoradiography S. Kaufmann 79 The use of quantum dot luminescent probes for Western blot analysis S. Makrides, C. Gasbarro and J.M.Bello 80 The use of infrared fluorescent dyes in quantitative immunoblotting C-H Yang, C. Kasbek, and H. A. Fisk 81 The use of infrared fluorescent dyes in immunofluorescence microscopy C. Kasbek, C-H Yang and H. A. Fisk Part IV : CHEMICAL MODIFICATION OF PROTEINS AND PEPTIDE PRODUCTION, PURIFICATION AND CHARACTERISATION 82 Carboxymethylation of Cysteine Using Iodoacetamide/Iodoacetic Acid A. Aitken and Michele Learmonth83 Performic Acid Oxidation A. Aitken and Michele Learmonth84 Succinylation of Protein A. Aitken and Michele Learmonth85 Pyridylethylation of Cysteine Residues M. Ward86. Side-Chain Selective Chemical Modifications of Proteins D. S. Tawfik87 Nitration of Tyrosines D. S. Tawfik88 Ethoxyformylation of Histidine D. S. Tawfik89 Modification of Arginine Side Chains with p-Hydroxyphenylglyoxal D. S. Tawfik90 Amidination of Carboxyl Groups D. S. Tawfik91 Amidination of Lysine Side Chains D.S. Tawfik92 Modification of tryptophan with 2-Hydroxy-5-Nitrogenzylbromide D. S. Tawfik 93. Modification of Sulhydryl Groups with DTNB D. S. Tawfik 94 Chemical Cleavage of Proteins at Methionyl-X Peptide Bonds B.J. Smith95 Chemical Cleavage of Proteins at Tryptophanyl-X Peptide Bonds B. J. Smith96 Chemical Cleavage of Proteins at Aspartyl-X Peptide Bonds B. J.Smith97 Chemical Cleavage of Proteins at Cysteinyl-X Peptide Bonds B. J. Smith98 Chemical Cleavage of Proteins at Asparaginyl-Glycyl Peptide Bonds B.J. Smith99 Enzymatic Digestion of Proteins in Solution and in SDS Polyacrylamide Gels K.L. Stone, E.E.Gulcicek and K.R. Williams100 On-PVDF protein digestions for N-terminal sequencing and peptide mass fingerprinting Victoria Pham, William Henzel & Jennie R Lill101 Enzymatic Digestion of Proteins on PVDF membranes J. Fernandez and S. Mische102 Reverse Phase HPLC Separation of Enzymatic Digests of Proteins K.L. Stone and K.R. Williams103 Peptide Mapping by Two-Dimensional Thin-Layer Electrophoresis-Thin-Layer Chromatography R. C. Judd104 Peptide Mapping by Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis R. C. Judd105 Peptide Mapping by High-Performance Liquid Chromatography P. Hojrup 106 Production of Protein Hydrolysates Using Enzymes J. M. Walker and P. J. Sweeney107 Amino Acid Analysis by Precolumn Derivatization with 1- Fluoro-2,4-Dinitrophenyl-5-L-Alanine Amide (Marfey's Reagent) S. Kochhar and P. Christen 108 Amino acid analysis in protein hydrolysates using anion exchange chromatography and IPAD detection P. Jandik, Jun Cheng and N. Avdalovic 109 Validation of amino acid analysis A. Reason110 Molecular Weight Estimation for Native Proteins Using High-Performance Size Exclusion Chromatography G. B. Irvine 111 Detection of Disulfide-Linked Peptides by HPLC A. Aitken and M. Learmonth112 Detection of Disulfide-Linked Peptides by Mass Spectrometry A.Aitken and M. Learmonth113 Diagonal Electrophoresis for Detecting Disulfide Bridges A. Aitken and M. Learmonth114. Estimation of Disulfide Bonds Using Ellman's Reagent A. Aitken and M. Learmonth115 Quantitation of Cysteine Residues and Disulfide Bonds by Electrophoresis A. Aitken and M. Learmonth116 N-Terminal sequencing of the N-terminally Modified Proteins R. Kamp and Hisashi Hirano 117 De-blocking of Proteins containing N-Terminal Pyroglutamic AcidcJ. Mozdzanowski 118 Detection and characterization of protein mutations by mass spectrometryY. Wada119 Peptide Sequencing by Nanoelectrospray Tandem Mass SpectrometryO. Norregaard Jensen and M. Wilm120 Protein identification by Peptide mass fingerprinting using MALDI-TOFF Mass spectrometryJ. Webster and D. Oxley 121 Protein ladder sequencing Rong Wang and Brian T. Chait122. Sequence Analysis with WinGene/WinPepL. Hennig 123 HPLC and MS of integral membrane proteinsJ. Whitelegge 124 Enrichment of Serum Peptides and Analysis by MALDI-TOF Mass Spectrometry H.W. Ressom, R.S.Varghese and R. Goldman125 Computational Methods for the Analysis of MALDI-TOF Spectra to Discover Peptide Serum Biomarkers Y. An, H.W. Ressom and R. Goldman Part V : POST-TRANSLATIONAL MODIFICATIONS 126 Simple tools for complex N-glycan analysis A-C. Fitchette, M. Benchabane, T. Paccalet, L. Faye and V. Gomord127 A Lectin-Binding Assay for the Rapid Characterization of the Glycosylation of Purified Gycoproteins M.T. Goodarzi, A. Fotinopoulou and G. A. Turner128 Chemical Methods of Analysis of Glycoproteins E.F. Hounsell, M.J. Davies and K.D. Smith129 Monosaccharide Analysis by HPAEC E.F. Hounsell, M.J. Davies and K.D. Smith130 Monosaccharide Analysis by GC E.F. Hounsell, M.J. Davies and K.D. Smith131 Determination of monosaccharide linkage and Substitution Patterns by GC.MS Methylation Analysis E.F. Hounsell, M.J. Davies and K.D. Smith132 Sialic Acid Analysis by HPAEC-PAD E.F. Hounsell, M.J. Davies and K.D. Smith133 Chemical Release of O-Linked Oligosaccharide Chains E.F. Hounsell, M.J. Davies and K.D. Smith134 O-Linked Oligosaccharide Profiling by HPLC E.F. Hounsell, M.J. Davies and K.D. Smith135 O-Linked Oligosaccharide Profiling by HPAEC-PAD E.F. Hounsell, M.J. Davies and K.D. Smith136 Release of N-Linked Oligosaccharide Chains by Hydrazinolysis T.Mizuochi and E.F. Hounsell137 Enzymatic Release of O- and N-Linked Oligosaccharide Chains E.F. Hounsell, M.J. Davies and K.D. Smith138 N-Linked Oligosaccharide Profiling by HPLC on Porous Graphitized Carbon (PGC) E.F. Hounsell, M.J. Davies and K.D. Smith139 N-linked Oligosaccharide Profiling by HPAEC-PAD E.F. Hounsell, M.J. Davies and K.D. Smith140 HPAE-PAD analysis of monosaccharides released by exoglycosidase digestion Weitzhandler, M., J. Rohrer, J,R Thayer and N. Avdalovic 141 Microassay Analyses of Protein Glycosylation N.K.C. Wong, N. Kanu, N. Thandrayen, G,J.Rademaker, C.I. Baldwin, D.V. Renouf and E.F. Hounsell 142 Polyacrylamide gel electrophoresis of fluorophore-labeled carbohydrates from glycoproteinsB. Brandley, J. Klock and C. Starr 143 HPLC Analysis of fluorescently labelled glycans A. Merry and S. Astrautsova144 Glycoprofiling purified glycoproteins using Surface Plasmon Resonance A. Fotinopoulou and G.A. Turner 145 Sequencing Heparan Sulphate Saccharides J. E. Turnbull146 Analysis of glycoprotein heterogeneity by Capillary Electrophoresis and Mass Spectrometry A. Hooker and D.C. James147 Affinity chromatography of oligosaccharides and glycopeptides with immobilized lectins K. Yamamoto 148 In-Gel Enzymatic Release of N-glycans D. Harvey149 Analysis of N-Linked glycans by mass spectrometry D. Harvey150 MS Analysis of Protein glycosylation N.Takemori, N. Komori and H. Matsumoto 151 Mapping protein N-glycosylation by COFRADIC B. Ghesquiere, J. Vandekerckhove, and K. Gevaert 152 Mass spectrometric analysis of O-linked glycans released directly from Glycoproteins in gels using ss-elimination A.M. Taylor and J. Thomas-Oates 153 Glycopeptide analysis using LC/MS and LC/MSn S.Itoh, D. Takakura, N. Kawasaki and T. Yamaguchi 154 Identification of Vitamin K-Dependent Proteins Using a Gla-specific Monoclonal Antibody K. Hansson 155 The identification of protein S-Nitrocysteine T.M Greco, S.L.Stamer, D.C. Liebler and H. Ischiropoulos 156 Detection of nitrotyrosine-containing proteins Xianquan Zhan and D. Desiderio 157 Mass spectrometric determination of protein Ubiquitination C. E. Parker, M.R.E. Warren, V. Mocanu, S.F. Greer and C.H. Borchers158 Detection of Sumoylated ProteinsOk-Kyong Park-Sarge and K. Sarge 159 Efficient enrichment of intact phosphorylated proteins by modified immobilized metal-affinity chromatographyA. Dubrovska 160 Analyzing Protein Phosphorylation J. Colyer161 Mass spectrometric analysis of protein phosphorylation S. Gander, A. Cremonesi, J. Chicher, S. Moes and P. Jenoe 162 Protein microarrays for phosphorylation studies B. Kersten and T. Feilner 163. Two Dimensional Phosphopeptide Mapping H. Nagahara, R.R. Latek, S.A. Ezhevsky and S.F. Dowdy 164 Identification of Proteins Modified by Protein (D-Aspartyl/L-Isoaspartyl) Carboxyl Methyltransferase D. Weber and P.N. McFadden165 Analysis of Tyrosine-o- sulfation Jens R. Bundgaard, J.W. Sen, A.H. Johnsen and J.F. Rehfeld 166 Analysis of Protein Palmitoylation by metabolic radiolabeling methods K.H. Pedone, L.S. Bernstein, Maurine E. Linder and J.R. Hepler167 Incorporation of radiolabeled prenyl alcohols and their analogs into mammalian cell proteins: a useful tool for studying protein prenylationA. Corsini, C.C. Farnsworth, P. McGeady, M.H. Gelb and J.A. Glomset168 Labelling and analysis of isoprenylated proteins in cells D.A. Andres, D.C. Crick, H.P.Spielmann and C. J. Waechter Part VII : ANTIBODY TECHNIQUES 169 Antibody Production R. Burns170 Production of Antibodies Using Proteins in Gel Bands S.A. Amero, T.C. James and S.C.R. Elgin171 Raising Highly Specific Polyclonal Antibodies Using Biocompatible Support Bound Antigens M. Diano and A. Le Bivic172 Production of antisera using peptide conjugates T. E. Adrian173 Small-molecule protein conjugation procedures S. Thompson 174 The Chloramine T Method for Radiolabeling Protein G.S. Bailey175 The Lactoperoxidase Method for Radiolabeling Protein G.S. Bailey176 The Bolton and Hunter Method for Radiolabeling Protein G.S. Bailey177 Preparation of 125I-labelled peptides and proteins with high specific activity using iodogen M. Conlon 178 Purification and Assessment of Quality of Radioiodinated Protein G.S. Bailey179 Purification of IgG by Precipitation with Sodium Sulfate or Ammoniun SulfateM. Page and R. Thorpe180 Purification of IgG Using Caprylic Acid M. Page and R. Thorpe181 Purification of IgG Using DEAE-Sepharose Chromatography M. Page and R. Thorpe182 Purification of IgG Using Ion-Exchange HPLC M. Page and R. Thorpe183 Purification of IgG by Precipitation with Polyethylene Glycol (PEG) M. Page and R. Thorpe184 Purification of IgG Using Protein A or Protein G M. Page and R. Thorpe185 Purification of IgG Using Size Exclusion High Performance Liquid Chromatography (SE-HPLC) C. Dolman and R. Thorpe 186 Purification of IgG Using Affinity Chromatography on Antigen-Ligand Columns M. Page and R. Thorpe187 Purification of IgG Using Thiophilic ChromatographyM. Page and R. Thorpe188 Analysis of IgG Fractions by Electrophoresis M. Page and R. Thorpe189 Purification of Immunoglobulin Y (IgY) from Chicken Eggs C.R. Bird and R. Thorpe 190 Affinity Purification of Immunoglobulins Using PAM (Protein A Mimetic)G. Fassina G. Palombo, A. Verdoliva and M. Ruvo191 Detection of Serological Cross Reactions by Western Cross Blotting P. Hemmerl, A. Hartl, J. Freund and J.Thalhamer 192 Enzymatic Digestion of Monoclonal Antibodies S. Andrew193 How to Make Bispecific Antibodies R. French194 Antigen Measurements Using ELISA W. Jordan195 Enhanced chemiluminescence immunoassay R. Stott196 Immunoprecipitation and Blotting S. Thompson 197 Determination of Epitope by Mass Spectrometry C.Hager-Braun and K. B. Tomer 198 Immunogen Preparation and Immunization Procedures for Rats and Mice M. Page and R. Thorpe199 Making Hybridomas R. Burns200 Growing hybridomas G. Entrican, C. Jepson and D. Deane201 Mouse Hybridomas as an Entryway to Monoclonal Antibody Design and Production E. Mechetner202 Pitfalls E. Mechetner203 Recombinant antibody expression and purification A. Knappik and R. Brundiers204 Screening Hybridoma Culture Supernatants Using Solid-Phase Radiobinding Assay M. Page and R. Thorpe205 Screening Hybridoma Culture Supernatants Using ELISA M. Page and R. Thorpe206 Growth and Purification of Murine Monoclonal Antibodies M. Page and R. Thorpe207 Affinity purification techniques for monoclonal antibodies A. Schwarz208 A rapid method for generating large numbers of high-affinity monoclonal antibodies from a single mouseN.T. Man and G.E. Morris
Responsibility: edited by John M. Walker.
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