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Protein Purification : Principles and Practice

Author: Robert K Scopes
Publisher: New York, NY : Springer New York, 1987.
Series: Springer advanced texts in chemistry.
Edition/Format:   eBook : Document : English : Second editionView all editions and formats
Summary:
This updated edition of a widely accepted textbook now also includes discussions of the important techniques of high performance liquid chromatography and dye-ligand chromatography. The book is a practical guide on how to get started in planning a protein isolation and provides enough information to permit an intelligent choice of appropriate techniques. By pointing to more detailed information in the literature, it  Read more...
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Genre/Form: Electronic books
Additional Physical Format: Print version:
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Robert K Scopes
ISBN: 9781475719574 1475719574
OCLC Number: 853271633
Description: 1 online resource (xv, 329 pages).
Contents: The Enzyme Purification Laboratory --
Making an Extract --
Separation by Precipitation --
Separation by Adsorption I. General Principles --
Separation by Adsorption II. Specific Adsorbents --
Separation in Solution --
Optimization of Procedures; Recombinant Protein Techniques --
Maintenance of Active Enzymes --
Measurement of Enzyme Activity --
Analysis for Purity: Crystallization --
Addition of Precipitants --
Solutions for Measuring Protein Concentration --
Appendices --
References --
Index.
Series Title: Springer advanced texts in chemistry.
Responsibility: by Robert K. Scopes.

Abstract:

This updated edition of a widely accepted textbook now also includes discussions of the important techniques of high performance liquid chromatography and dye-ligand chromatography. The book is a practical guide on how to get started in planning a protein isolation and provides enough information to permit an intelligent choice of appropriate techniques. By pointing to more detailed information in the literature, it can also act as a reference book. This textbook is aimed at courses in protein purification in biochemistry and downstream processing in biotechnology.

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