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Protein sequencing protocols

Author: Bryan John Smith
Publisher: Totowa, N.J. : Humana Press, ©2003.
Series: Methods in molecular biology (Clifton, N.J.), v. 211.
Edition/Format:   Print book : English : 2nd edView all editions and formats
Summary:

Determination of the protein sequence is as important today as it was a half century ago, even though the techniques and purposes have changed over time. The first edition of Protein Sequencing  Read more...

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Genre/Form: Laboratory manuals
Aufsatzsammlung
Laboratory Manuals
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: Bryan John Smith
ISBN: 0896039757 9780896039759
OCLC Number: 123154440
Description: xii, 493 pages : illustrations ; 24 cm.
Contents: 1. Strategies for Handling Polypeptides on a Micro-Scale / Bryan John Smith and Paul Tempst --
2. SDS Polyacrylamide Gel Electrophoresis for N-Terminal Protein Sequencing / Bryan John Smith --
3. Two-Dimensional Polyacrylamide Gel Electrophoresis for the Separation of Proteins for Chemical Characterization / Michael J. Dunn --
4. Electroblotting of Proteins from Polyacrylamide Gels for Chemical Characterization / Michael J. Dunn --
5. Enzymatic Cleavage of Proteins / Bryan John Smith --
6. Chemical Cleavage of Polypeptides / Bryan John Smith --
7. Enzymatic Preparation and Isolation of Glycopeptides / David J. Harvey --
8. Analytical and Micropreparative Capillary Electrophoresis of Peptides / Alan J. Smith --
9. High-Performance Liquid Chromatography On-Line Derivative Spectroscopy for the Characterization of Peptides with Aromatic Amino Acid Residues / Christoph W. Turck --
10. Hydrolysis of Samples for Amino Acid Analysis / Ian Davidson --
11. Amino Acid Analysis: Precolumn Derivatization Methods / G. Brent Irvine and Ian Davidson --
12. Post Column Amino Acid Analysis / Alan J. Smith --
13. Amino Acid Analysis Using Pre-Column Derivatization with 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate: Analysis of Hydrolyzed Proteins and Electroblotted Samples / Steven A. Cohen --
14. Amino Acid Analysis in Protein Hydrolysates Using Anion Exchange Chromatography and IPAD Detection / Petr Jandik, Jun Cheng and Nebojsa Avdalovic --
15. D-Amino Acid Analysis / Andrea Scaloni, Maurizio Simmaco and Francesco Bossa --
16. Validation of Amino Acid Analysis Methods / Andrew J. Reason --
17. Electrospray Mass Spectrometry of Peptides, Proteins, and Glycoproteins / Fiona M. Greer and Howard R. Morris --
18. Peptide Mass Fingerprinting Using MALDI-TOF Mass Spectrometry / Darryl J. C. Pappin --
19. Nanoelectrospray Tandem Mass Spectrometry and Sequence Similarity Searching for Identification of Proteins from Organisms with Unknown Genomes / Anna Shevchenko, Shamil Sunyaev, Adam Liska, Peer Bork and Andrej Shevchenko --
20. Direct Identification of Proteins in Ultracomplex Mixtures: Applications to Proteome Analysis / David M. Schieltz and John R. Yates III --
21. Identification of PTH-Amino Acids by HPLC / Gregory A. Grant and Mark W. Crankshaw --
22. Protein Sequencer Maintenance and Troubleshooting / Bryan Dunbar --
23. N-Terminal Protein Sequencing for Special Applications / Philip J. Jackson --
24. Identification of Phosphorylation Sites by Edman Degradation / Laurey Steinke and Richard G. Cook --
25. Validation of Protein Sequencing in a Regulated Laboratory / Philip J. Jackson and Neil Dodsworth --
26. Automated C-Terminal Protein Sequence Analysis Using the Alkylated-Thiohydantoin Method / David R. Dupont, Sylvia W. Yuen and Kenneth S. Graham --
27. Chemical Modifications of Proteins as an Aid to Sequence Analysis / Alex F. Carne --
28. Deblocking of N-Terminally Modified Proteins / Hisashi Hirano and Roza M. Kamp --
29. Deblocking of Proteins Containing N-Terminal Pyroglutamic Acid / Jacek Mozdzanowski --
30. Identification of Sites of Glycosylation / David J. Harvey --
31. Analysis of Sites of Protein Phosphorylation / Alastair Aitken and Michele Learmonth --
32. Quantitation and Location of Disulfide Bonds in Proteins / Alastair Aitken and Michele Learmonth --
33. Getting the Most from Your Protein Sequence / Richard R. Copley and Robert B. Russell --
App. 1. Letter Codes, Structures, Masses, and Derivatives of Amino Acids / James P. Turner --
App. 2. Protein Consensus Sequence Motifs / Alastair Aitken.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 211.
Responsibility: edited by Bryan John Smith.
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"...this book would certainly benefit readers who are new to the field as well as those more experienced as it serves as a convenient collection of tried and trusted methods...the book gives Read more...

 
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