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Proteoglycan protocols

Author: Renato V Iozzo
Publisher: Totowa, NJ : Humana Press, 2001.
Series: Methods in molecular biology (Clifton, N.J.), v. 171.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Database:WorldCat
Summary:
Proteoglycans, highly complex macromolecules found in mammalian and lower organisms, serve a multitude of biological functions. In Proteoglycan Protocols, Renato Iozzo, MD, and an authoritative team of investigators present for the first time a comprehensive and up-to-date collection of readily reproducible preparative and analytical methods for the in-depth analysis of these important compounds. Featuring  Read more...
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Genre/Form: Laboratory Manuals
Electronic books
Laboratory manuals
Manuels de laboratoire
Additional Physical Format: Print version:
Proteoglycan protocols.
Totowa, NJ : Humana Press, 2001
(DLC) 00061317
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Renato V Iozzo
ISBN: 0585403198 9780585403199 1592592090 9781592592098
OCLC Number: 50175101
Reproduction Notes: Electronic reproduction. [S.l.] : HathiTrust Digital Library, 2010. MiAaHDL
Description: 1 online resource (xvi, 558 pages) : illustrations.
Details: Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.
Contents: Isolation of proteoglycans from cell cultures and tissues / M. Yanagishita --
Isolation of proteoglycans from tendon / K.G. Vogel and J.A. Peters --
Purification of proteoglycans from mineralized tissues / N.S. Fedarko --
Purification of perlecan from endothelial cells / J. Whitelock --
Isolation and characterization of nervous tissue proteoglycans / Y. Yamaguchi --
Analysis of proteoglycans and glycosaminoglycans from drosophila / W.D. Staatz [and others] --
Cartilage and smooth muscle cell proteoglycans detected by affinity blotting using biotinylated hyaluronan / J. Melrose --
Preparation of proteoglycans for N-terminal and internal amino acid sequence analysis / P.J. Neame --
High-specific-activity 35S-labeled heparan sulfate prepared from cultured cells / N.W. Shworak --
Selective detection of sulfotransferase isoforms by the ligand affinity-conversion approach / N.W. Shworak --
Particulate and soluble glycosaminoglycan-synthesizing enzymes. Preparation assay and use / G. Sugumaran and J.E. Silbert --
Disaccharide composition of hyaluronan and chondroitin/dermatan sulfate. Analysis with fluorophore-assisted carbohydrate electrophoresis / A.H. Plaas [and others] --
Integral glycan sequencing of heparan sulfate and heparin saccharides / J.E. Turnbull --
Analytical and preparative strong anion-exchange HPLC of heparan sulfate and heparin saccharides / J.E. Turnbull --
Proteoglycans analyzed by composite gel electrophoresis and immunoblotting / G.R. Dodge and R. Heimer --
Quantitation of proteoglycans in biological fluids using alcian blue / M. Karlsson and S. Bjornsson --
Cellulose acetate electrophoresis of glycosaminoglycans / Y. Wegrowski and F.X. Maquart --
Disaccharide composition in glycosaminoglycans/proteoglycans analyzed by capillary zone electrophoresis / N.K. Karamanos and A. Hjerpe --
Intact and oligomeric glycosaminoglycans analyzed with capillary electrophoresis / N.K. Karamanos and A. Hjerpe --
Recombinant expression of proteoglycans in mammalian cells. Utility and advantages of the vaccinia virus/T7 bacteriophage hybrid expression system / D.J. McQuillan [and others] --
Purification of recombinant human decorin and its subdomains / A.L. McBain and D.M. Mann --
Prokaryotic expression of proteoglycans / A.D. Murdoch and R.V. Iozzo --
Proteoglycan gene targeting in somatic cells / G. Ghiselli and R.V. Iozzo --
Constitutive and inducible antisense expression / M. Handler and R.V. Iozzo --
Cell-mediated transfer of proteoglycan genes / J.W. Fischer [and others] --
Morphological evaluation of proteoglycans in cells and tissues / S.L. Lara, S.P. Evanko and T.N. Wight --
Expression and characterization of engineered proteoglycans / W. Luo [and others] --
Intracellular localization of engineered proteoglycans / T.L. Chen [and others] --
Selection of glycosaminoglycan-deficient mutants / X. Bai, B. Crawford and J.D. Esko --
Xyloside priming of glycosaminoglycan biosynthesis and inhibition of proteoglycan assembly / T.A. Fritz and J.D. Esko --
Inhibition of heparan sulfate synthesis by chlorate / H.E. Conrad --
Detection of proteoglycan core proteins with glycosaminoglycan lyases and antibodies / J.R. Couchman and P. Tapanadechopone --
Proteoglycan core proteins and catabolic fragments present in tissues and fluids / J.D. Sandy --
Degradation of heparan sulfate by nitrous acid / H.E. Conrad --
Degradation of heparan sulfate with heparin lyases / L.A. LeBrun and R.J. Linhardt --
Degradation of chondroitin sulfate and dermatan sulfate with chondroitin lyases / M.J. Hernaiz and R.J. Linhardt --
In vitro assays for hyaluronan synthase / A.P. Spicer --
Hyaluronidase activity and hyaluronidase inhibitors. Assay using a microtiter-based system / S.S. Nawy [and others] --
Detecting hyaluronidase and hyaluronidase inhibitors. Hyaluronan-substrate gel and -inverse substrate gel techniques / K. Mio [and others] --
Affinity coelectrophoresis of proteoglycan-protein complexes / J.D. San Antonio and A.D. Lander --
Binding constant measurements for inhibitors of growth factor binding to heparan sulfate proteoglycans / K.E. Forsten and M.A. Nugent --
Interaction of proteoglycans with receptor tyrosine kinases / D.K. Moscatello and R.V. Iozzo --
Measuring single-cell cytosolic ca2+ concentration in response to proteoglycans / S. Patel and R.V. Iozzo --
Serum amyloid a peptide interactions with glycosaminoglycans. Evaluation by affinity chromatography / J.B. Ancsin and R. Kisilevsky --
Interactions of lipoproteins with proteoglycans / K.J. Williams --
Hyaluronan and hyaluronan-binding proteins. Probes for specific detection / B.P. Toole, Q. Yu and C.B. Underhill --
Novel confocal-FRAP analysis of carbohydrate-protein interactions within the extracellular matrix / P. Gribbon, B.C. Heng and T.E. Hardingham --
Regulatory roles of syndecans in cell adhesion and invasion / J.K. Langford and R.D. Sanderson --
Optical biosensor techniques to analyze protein-polysaccharide interactions / D.G. Fernig --
Phage display technology to obtain antiheparan sulfate antibodies / T.H. van Kuppevelt [and others] --
Tissue-specific binding by FGF and FGF receptors to endogenous heparan sulfates / A. Friedl, M. Filla and A.C. Rapraeger.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 171.
Responsibility: edited by Renato V. Iozzo.

Abstract:

Presents a comprehensive collection of reproducible preparative and analytical methods for the in-depth analysis of these important compounds. Featuring protocols, this book enables both novice and  Read more...

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"The book is strategically subdivide into 3 sections (isolation and purification; expression, detection, and degradation; interactions) with a total of 51 chapters ( 19, 20, and 12 per subheading). Read more...

 
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