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Proteomics in practice : a laboratory manual of proteome analysis

Author: Reiner Westermeier; Tom Naven
Publisher: Weinheim : Wiley-VCH, ©2002.
Edition/Format:   Print book : EnglishView all editions and formats
Summary:

Proteomics is a key area in the post-genomic era providing new insights into protein functions, interactions, and pathways. It is equally important for basic as well as applied research. This  Read more...

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Genre/Form: Laboratory Manuals
Handbooks and manuals
Handbooks, manuals, etc
Additional Physical Format: Online version:
Westermeier, Reiner.
Proteomics in practice.
Weinheim : Wiley-VCH, ©2002
(OCoLC)606840471
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: Reiner Westermeier; Tom Naven
ISBN: 9783527303540 3527303545
OCLC Number: 55689485
Description: xxiv, 316 pages : illustrations ; 25 cm
Contents: pt. I. Proteomics Technology --
Introduction --
Expression proteomics --
Two-dimensional Electrophoresis --
Spot handling --
Mass spectrometry --
Protein identification by database searching --
Methods of proteome analysis --
pt. II. Course Manual --
Step 1. Sample preparation --
Step 2. Isoelectric focusing --
Step 3. SDS Polyacrylamide Gel Electrophoresis --
Step 4. Staining of the gels --
Step 5. Scanning of gels and image analysis --
Step 6. Fluorescence difference gel electrophoresis --
Step 7. Spot excision --
Step 8. Sample destaining --
Step 9. In-gel digestion --
Step 10. Microscale desalting and concentration of sample --
Step 11. Chemical derivatisation of the peptide digest --
Step 12. MS analysis --
Step 13. Calibration of the MALDI-ToF MS --
Step 14. Preparing for a database search --
Step 15. PMF database search unsuccessful.
Responsibility: Reiner Westermeier, Tom Naven.
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"This book says straight from the start that it will tell you how to do proteomics by 2D electrophoresis, followed by peptide mass fingerprinting, and then CAF MALDI sequence analysis" Read more...

 
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