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Receptor signal transduction protocols

Author: Gary B Willars; R A J Challiss
Publisher: New York : Springer, ©2011.
Series: Springer protocols (Series); Methods in molecular biology (Clifton, N.J.), v. 746.
Edition/Format:   Print book : English : 3rd edView all editions and formats
Summary:
[Publisher-supplied data] As our understanding of G protein-coupled receptor (GPCR) signal transduction continues to grow, we cannot help but be struck by the emerging complexity and the ability of this receptor superfamily to continually surprise us as new facets are discovered. In this third edition of Receptor Signal Transduction Protocols, the expert contributors have taken into account the constant evolution of  Read more...
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Genre/Form: Aufsatzsammlung
Laboratory Manuals
Additional Physical Format: Online version:
Receptor signal transduction protocols.
New York : Humana Press, ©2011
(OCoLC)730973427
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: Gary B Willars; R A J Challiss
ISBN: 1617791253 9781617791253 9781617791260 1617791261
OCLC Number: 706784502
Description: xi, 479 pages : illustrations (some color) ; 27 cm.
Contents: Inducible expression of G protein-coupled receptors in transfected cells / Beryl Koener and Emmanuel Hermans --
Using the Flp-In T-Rex system to regulate GPCR expression / Richard J. Ward, Elisa Alvarez-Curto, and Graeme Milligan --
Viral infection for GPCR expression in eukaryotic cells / Antonio Porcellini, Luisa Iacovelli, and Antonio De Blasi --
Generation of epitope-tagged GPCRs / Yan Huang and Gary B. Willars --
Use of site-directed mutagenesis to study GPCRs / Alex C. Conner [and others] --
Approaches to study GPCR regulation in native systems / Jonathon M. Willets --
Heterologous expression of GPCRs in fission yeast / John Davey and Graham Ladds --
Radioligand binding methods for membrane preparations and intact cells / David B. Bylund and Myron L. Toews --
Quantification of GPCR mRNA using real-time RT-PCR / Trond Brattelid and Finn Olav Levy --
Determining allosteric modulator mechanism of action : integration of radioligand binding and functional assay data / Christopher J. Langmead --
Design and use of fluorescent ligands to study ligand-receptor interactions in single living cells / Stephen J. Briddon, Barrie Kellam, and Stephen J. Hill --
Examining site-specific GPCR phosphorylation / Adrian J. Butcher, Andrew B. Tobin, and Kok Choi Kong --
Ubiquitination of GPCRs / Adriana Caballero and Adriano Marchese --
[35S]GTPgS binding as an index of total G-protein and Ga-subtype-specific activation by GPCRs / Rajendra Mistry, Mark R. Dowling, and R.A. John Challiss --
Using calcium imaging as a readout of GPCR activation / Martin D. Bootman and H. Llewelyn Roderick --
Measuring spatiotemporal dynamics of cyclic AMP signaling in real-time using FRET-based biosensors / Frank Gesellchen [and others] --
Determining the activation of Rho as an index of receptor coupling to G12/13 proteins / Michio Nakaya [and others] --
Use of translocating fluorescent biosensors for real-time monitoring of GPCR-mediated signaling events / Carl P. Nelson and R.A. John Challiss --
Study of GPCR-protein interactions using gel overlay assays and glutathione-S-transferase-fusion protein pull-downs / Ashley E. Brady [and others] --
Study of GPCR-protein interactions by BRET / Martina Kocan and Kevin D.G. Pfleger --
Time resolved FRET strategy with fluorescent ligands to analyze receptor interactions in native tissues : application to GPCR oligomerization / Martin Cottet [and others] --
Peptide affinity purification for the isolation and identification of GPCR-associated protein complexes / Pascal Maurice, Avais M. Daulat, and Ralf Jockers --
Tandem affinity purification and identification of GPCR-associated protein complexes / Avais M. Daulat, Pascal Maurice, and Ralf Jockers --
Identification of GPCR localization in detergent resistant membranes / Ranju Kumari and Anna Francesconi --
Analysis of GPCR localization and trafficking / James N. Hislop and Mark von Zastrow --
Statistical methods in research / Domenico Spina.
Series Title: Springer protocols (Series); Methods in molecular biology (Clifton, N.J.), v. 746.
Responsibility: edited by Gary B. Willars and R.A. John Challiss.
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Abstract:

In this third edition of Receptor Signal Transduction Protocols, the expert contributors take into account the constant evolution of the GPCR field. It contains detailed revisions of chapters from  Read more...

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From the reviews of the third edition:"This is an extensive collection of established, new, and updated techniques for the study of the consequences of receptor activation. Each of the 26 chapters Read more...

 
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