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Recombinant DNA Laboratory Manual, Revised Edition.

Author: Judith W Zyskind; Sanford I Bernstein
Publisher: Oxford : Elsevier Science, 1992.
Edition/Format:   eBook : Document : English
Summary:
The latest edition of this introductory benchtop manual is up-to-date, affordable, and easy-to-follow. This text is perfect for your two-quarter or one semester course in Recombinant DNA Techniques and is specifically designed to lead your student or technician, who is a newcomer to molecular biology, from the basic skills of growing and maintaining bacterial colonies through plasmid DNA isolation, cloning, DNA  Read more...
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Genre/Form: Electronic books
Laboratory manuals
Additional Physical Format: Print version:
Zyskind, Judith W.
Recombinant DNA Laboratory Manual, Revised Edition.
Oxford : Elsevier Science, ©1992
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Judith W Zyskind; Sanford I Bernstein
ISBN: 9780323137874 0323137873
OCLC Number: 829460322
Notes: Day 3. Hybridization.
Description: 1 online resource (239 pages)
Contents: Front Cover; Copyright Page; Recombinant DNA Laboratory Manual; Dedication; Table of Contents; PREFACE; SCHEDULE OF LABORATORY EXERCISES; Lab I. BACTERIAL GROWTH PARAMETERS; Day 1. Measuring Bacterial Cell Growth; Day 2. Plotting Cell Growth Data; Addendum; Lab II. Isolation and Analysis of Bacterial and Drosophila Chromosomal DNA; Day 1. Isolation and Purification of E. coli Chromosomal DNA; Day 2. Determination of the Concentration and Purity of DNA by UV Spectroscopy; Day 3. Restriction Endonuclease Digestion of Chromosomal DNA. Day 4. Agarose Gel of Chromosomal DNA Restriction Endonuclease DigestionsDay 5. Staining and Photography of Agarose Gel of Chromosomal DNA Restriction Endonuclease Digestions; Lab III. Plasmid DNA Isolation and Agarose Gel Analysis; Day 1. Isolation of Plasmid DNA by the Alkaline-Detergent Method- A Miniprep Procedure; Day 2. Agarose Gel Electrophoresis of Undigested Plasmid DNA; Lab IV. Introduction of DNA into Cells; Day 1. Production of Frozen Competent Cells; Day 2. Transformation of LE392 with pBR329 DNA Isolated from HB101 ::Tn5; Lab V. Tn5 Mutagenesis of PBR329. Day 1. Marker Screening: Divide Transformants into Tcs and Tcr ClassesDay 2. Purification of Tcs and Tcr Clones; Day 3. Isolation of Plasmid DNA by the Alkaline-Detergent Method and Determination of Recovery by Agarose Gel Electrophoresis; Day 4. Restriction Mapping of the Tn5 Inserts using Pstl and EcoRl; Day 5. Agarose Gel of Plasmid DNA Restriction Endonuclease Digestions; Lab VI. DNA Cloning in M13; Day 1. Isolation of Restriction Fragment from an Agarose Gel; Day 2. Estimation of Recovery of Restriction Fragment and Isolation of M13mp 19 RF DNA. Day 3. EcoRl Digestion of M13mpl9 RF DNA and Treatment with Alkaline PhosphataseDay 4. Removing the Phosphatase and EcoRl and Analysis of the EcoRl Digest of M13mpl9 RF DNA; Day 5. Ligation; Ligation of EcoRl Digested M13mpl9 RF DNA and the Purified pRSG192 EcoRl Fragment; Day 6. Transfection of XL 1-Blue with Ligation Mixtures; Day 7. Plaque Purification; Day 8. Isolation of Colorless Plaques That Contain the chb Gene and Growth of Recombinant Bacteriophage; Lab VII. DNA Sequencing; Day 1. Isolation of Recombinant M13mpl9 RF and ss DNA. Day 2. Restriction Digestion and Gel Electrophoresis of Recombinant Phage to Determine Orientation of InsertEstimating the Recovery of ss DNA using Agarose Gel Electrophoresis; Day 3. Staining Gel of Pstl Restriction Fragments; Template Annealing and Dideoxy Sequencing; Day 4. Electrophoretic Separation of Sequencing Reactions; Day 5. Developing Autoradiogram and Reading DNA Sequence; Lab VIII. DNA Gel Blotting, Probe Preparation, Hybridization, and Hybrid Detection; Day 0. Agarose Gel Electrophoresis; Day 1. Gel Blotting; Day 2. Baking the Blot, Nick Translation, and Biotinylation of DNA.

Abstract:

The latest edition of this introductory benchtop manual is up-to-date, affordable, and easy-to-follow. This text is perfect for your two-quarter or one semester course in Recombinant DNA Techniques and is specifically designed to lead your student or technician, who is a newcomer to molecular biology, from the basic skills of growing and maintaining bacterial colonies through plasmid DNA isolation, cloning, DNA sequencing, and hybrid detection. The most useful features of this manual for your classroom and laboratory are:* Comb-bound, three-column, large 9-1/4"" x 7-1/2"" format.

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