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Recombinant DNA methodology

Author: Ray Wu; Lawrence Grossman; Kivie Moldave
Publisher: San Diego : Academic Press, ©1989.
Series: Selected methods in enzymology.
Edition/Format:   Print book : EnglishView all editions and formats

Covers topics such as: Enzymes in DNA research; DNA isolation, hybridization, and cloning; DNA sequence analysis; cDNA cloning; Gene products; Identification of cloned genes and mapping of genes;  Read more...


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Genre/Form: Collected Works
Additional Physical Format: Online version:
Recombinant DNA methodology.
San Diego : Academic Press, ©1989
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: Ray Wu; Lawrence Grossman; Kivie Moldave
ISBN: 0127655603 9780127655604
OCLC Number: 19064333
Notes: Reprint of articles from volumes 68, 100, 101, 153, 154, and 155 of Methods in enzymology.
Description: xxxi, 760 pages : illustrations ; 25 cm.
Contents: Enzymes in Recombinant DNA Research:N.P. Higgins and N.R. Cozzarelli, DNA-Joining Enzymes: A Review.R. Fuchs and R. Blakesley, Guide to the Use of Type II Restriction Endonucleases.M. McClelland, Site-Specific Cleavage of DNA at 8-, 9- and 10bp Sequences.L.-H. Guo and R. Wu, Exonuclease III: Use for DNA Sequence Analysis and in Specific Deletions of Nucleotides.Methods for Isolation, Purification, or Amplification of DNA:E. Southern, Gel Electrophoresis of Restriction Fragments.C.L. Smith and C.R. Cantor, Purification, Specific Fragmentation, and Separation of Large DNA Molecules.G.F. Carle and M.V. Olson, Orthogonal-Field-Alternation Gel Electrophoresis.H.C. Birnboim, A Rapid Alkaline Extraction Method for the Isolation of Plasmid DNA.K.B. Mullis and F.A. Faloona, Specific Synthesis of DNA in Vitro via a Polymerase-Catalyzed Chain Reaction.Vectors or Methods for Gene Cloning:J. Collins, Escherichia coli Plasmids Packageable in Vitro in a ~gl Bacteriophage Particles.J. Vieira and J. Messing, Production of Single-Stranded Plasmid DNA.H. Okayama, M. Kawaichi, M. Brownstein, F. Lee, T. Yokota, and K. Arai, High-Efficiency Cloning of Full-Length cDNA; Construction and Screening of cDNA Expression Libraries for Mammalian Cells.D.A. Morrison, Transformation and Preservation of Competent Bacterial Cells by Freezing.D. Hanahan and M. Meselson, Plasmid Screening at High Colony Density.J. Karn, S. Brenner, and L. Barnett, New Bacteriophage Lambda Vectors with Positive Selection for Cloned Inserts.A.M. Frischauf, N. Murray, and H. Lehrach, ~gl Phage Vectors-EMBL Series.M. Snyder, S. Elledge, D. Sweetser, R.A. Young, and R.W. Davis, ~glgt 11: Gene Isolation with Antibody Probes and Other Applications:R.J. Rothstein, One-Step Gene Disruption in Yeast.S.W. Rudy, J.W. Szostak, and A.W. Murrayv, Cloning Regulated Yeast Genes from a Pool of lacZ Fusions.L. Clarke, C.-L. Hsiao, and J. Carbon, Selection Procedure for Isolation of Centromere DNAs from Saccharomyces cerevisiae.J.R. Broach, Construction of High Copy Yeast Vectors Using 2-~gmm Circle Sequences.S.G. Rogers, H.J. Klee, R.B. Horsch, and R.T. Fraley, Improved Vectors for Plant Transformation: Expression Cassette Vectors and New Selectable Markers.Vectors or Methods for Expression of Cloned Genes:M.J. Casadaban, A. Martinez-Arias, S.K. Shapira, and J. Chou, ~gb-Galactosidase Gene Fusions for Analyzing Gene Expression in Escherichia coli and Yeast.M. Rosenberg, Y.-S. Ho, and A. Shatzman, The Use of pKC30 and Its Derivatives for Controlled Expression of Genes.E. Remaut, A. Marmenout, G. Simons, and W. Fiers, Expression of Heterologous Unfused Protein in Escherichia coli.G.D. Duffaud, P.E. March, and M. Inouye, Expression and Secretion of Foreign Proteins in Escherichia coli.L. Guarente, Yeast Promoters and LacZ Fusions Designed to Study Expression of Cloned Genes in Yeast.J.B. Gurdon and M.P. Wickens, The Use of Xenopus Oocytes for the Expression of Cloned Genes.G.A. Bitter, K.M. Egan, R.A. Koski, M.O. Jones, S.G. Elliott, and J.C. Giffin, Expression and Secretion Vectors for Yeast.Methods for Oligonucleotide-Directed Mutagenesis:M.J. Zoller and M. Smith, Oligonucleotide-Directed Mutagenesis of DNA Fragments Cloned into M13 Vectors.W. Kramer and H.-J. Fritz, Oligonucleotide-Directed Construction of Mutations via Gapped Duplex DNA.T.A. Kunkel, J.D. Roberts and R.A. Zakour, Rapid and Efficient Site-Specific Mutagenesis without Phenotypic Selection.R. Pine and P.C. Huang, An Improved Method to Obtain a Large Number of Mutants in a Defined Region of DNA.D.E. Hill, A.R. Oliphant, and K. Struhl, Mutagenesis with Degenerate Oligonucleotides: An Efficient Method for Saturating a Defined DNA Region with Base Pair Substitutions.Miscellaneous Methods:A. Vainstein, A. Razin, A. Graessmann, and A. Loyter, Fusogenic Reconstituted Sendai Virus Envelopes as a Vehicle for Introducing DNA into Viable Mammalian Cells.J.L. Manley, A. Fire, M. Samuels, and P.A. Sharp, In Vitro Transcriptions: Whole-Cell Extract.W.C. Merrick, Translation of Exogenous mRNAs in Reticulocyte Lysates.C.W. Anderson, J.W. Straus, and B.S. Dudock, Preparation of a Cell-Free Protein-Synthesizing System from Wheat Germ.R.D. Shillito and I. Potrykus, Direct Gene Transfer to Protoplasts of Dicotyledonous and Monocotyledonous Plants by a Number of Methods, Including Electroporation.R.M.K. Dale and A. Arrow, A Rapid Single-Stranded Cloning, Sequencing, Insertion, and Deletion Strategy.T.D. Tullius, B.A. Dombroski, M.E.A. Churchhill, and L. Kam, Hydroxyl Radical Footprinting: A High-Resolution Method for Mapping Protein-DNA Contacts.
Series Title: Selected methods in enzymology.
Other Titles: Methods in enzymology.
Responsibility: edited by Ray Wu, Lawrence Grossman, Kivie Moldave.
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