• Cite/Export
  Cite/Export

  Copy a citation

  APA (6th ed.)

  

  Chicago (Author-Date, 15th ed.)

  

  Harvard (18th ed.)

  

  MLA (7th ed.)

  

  Turabian (6th ed.)

  

  Export a citation

  Export to RefWorks   Export to EndNote / Reference Manager   Export to EasyBib   Cancel

  Note: Citations are based on reference standards. However, formatting rules can vary widely between applications and fields of interest or study. The specific requirements or preferences of your reviewing publisher, classroom teacher, institution or organization should be applied.
• Print
• E-mail
  E-mail

  All fields are required.

  The E-mail Address(es) field is required. Please enter recipient e-mail address(es).

  The E-mail Address(es) you entered is(are) not in a valid format. Please re-enter recipient e-mail address(es).

  You may send this item to up to five recipients.

  Enter recipient e-mail address(es): Separate up to five addresses with commas (,)

  The name field is required. Please enter your name.

  Enter your name: The subject field is required. Please enter the subject. Subject:

  The E-mail message field is required. Please enter the message.

  E-mail Message: I thought you might be interested in this item at http://www.worldcat.org/oclc/38007210 Title: The regular mosquito iridescent virus (RMIV) in the black saltmarsh mosquito, Aedes taeniorhynchus (Wiedemann): production, purification, transovarial transmission, site of entry, development of infections, and polypeptide composition Author: Stephen Carson Hembree Publisher: 1974. OCLC:38007210

  
  

  The ReCaptcha terms you entered were incorrect. Please try to match the 2 words shown in the window, or try the audio version.

  Cancel
• Share
• Permalink
  Permalink

  Copy this URL to link to this page:

• Write a review
• Rate this item:
• • 1
  • 2
  • 3
  • 4
  • 5

More like this

Subjects

• Viruses.
• Mosquitoes.

Similar Items

The regular mosquito iridescent virus (RMIV) in the black saltmarsh mosquito, Aedes taeniorhynchus (Wiedemann): production, purification, transovarial transmission, site of entry, development of infections, and polypeptide composition

Author:	Stephen Carson Hembree
Dissertation:	Thesis (Ph. D.)--University of Florida, 1974.
Edition/Format:	Thesis/dissertation : Thesis/dissertation : Manuscript  Archival Material : EnglishView all editions and formats
Summary:	A system permitting the weekly production by 1 person of gram quantities of the regular mosquito iridescent virus (RMIV) in the black saltmarsh mosquito, Aedes taeniorhynchus (Wiedemann) , was described. The efficiency of the system resulted from exposure of larvae at their most susceptible age to an optimum quantity of RMIV inoculum and from the use of a virus purification procedure designed to minimize waste. Transmission rates of about 10 per cent were routinely achieved. An average of 58 ug of virus was produced per infected larva, constituting 9.35 per cent of the dry weight of the average infected larva. Attenuation of infectivity occurred throughout the virus purification procedure, no major part of it being attributable to a single cause. Transovarial transmission of the virus increased with the age of larvae at. exposure through day 5 of larval life, but decreased when exposure was made just before pupation. All of the larvae produced by 9 isolated, transovarially transmitting females were infected. Transmitting females produced fewer average progeny than nontransmitting females. Males did not transmit virus to previously unexposed females. Total transmission (i.e., transmission resulting in overt disease plus transmission indicated only by infections among progeny) decreased with the age of larvae at exposure and was greatest for larvae in the second instar when exposed. A probable site of entry of RMIV into host tissue was found by eiectronmicroscopy in midgut epithelium at the level of the foregut invagination. Infections were found in fatbody, epidermis, imaginal buds and tracheal epithelium by autoradiography of 3H-methyl thymidine treated larvae 84 hr after the initiation of exposure to the virus. Infections were found in fatbody by fluorescent antibody staining 48 hr after initiation of exposure. Infection had appeared in epidermis and in imaginal buds by 72 hr after initiation of exposure, and in tracheal epithelium by 96 hr after initiation of exposure. Infections resulting in transovarial transmission could not be detected by fluorescent antibody staining. Twenty-five polypeptides were found in RMIV by polyacrylamide gel disc electrophoresis of sodium dodecyl sulfate---2-mercaptoethanol disrupted virus. The molecular weights of these were estimated by polyacrylamide gel disc electrophoresis and ranged from 23,500 to 331,000. Fourteen per cent of the genome of the virus could code for its structural polypeptides, assuming no redundancy and that no 5S ribosomal RNA or transfer RNA were coded for.  Read more...
Rating:	(not yet rated) 0 with reviews - Be the first.