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Regulators and effectors of small GTPases. / Part E, GTPases involved in vesicular traffic

Author: William Edward Balch; Channing J Der; Alan Hall, biochimiste)
Publisher: San Diego : Academic Press, cop. 2001.
Series: Methods in enzymology (Print), v. 329.
Edition/Format:   Print book : EnglishView all editions and formats
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Small GTPases play a key role in many aspects of contemporary cell biology: control of cell growth and differentiation; regulation of cell adhesion and cell movement; the organization of the actin  Read more...

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Document Type: Book
All Authors / Contributors: William Edward Balch; Channing J Der; Alan Hall, biochimiste)
ISBN: 0121822303 9780121822309
ISSN:0076-6879
OCLC Number: 490457661
Description: XXXVIII-553 p., [3] f. de pl. : ill. en coul. ; 24 cm.
Contents: Section I. Rab GTPases 1: Structural Basis for Rab Function: An Overview 2: Expression of Wild-Type and Mutant Green Fluorescent Protein-Rabl for Fluorescence Microscopy Analysis 3: Fluorescence Methods for Monitoring Interactions of Rab Proteins with Nucleotides, Rab Escort Protein, and Geranylgeranyltransferase 4: Prenylation of Rab Proteins in Vitro by Geranylgeranyltransferases 5: Antibody and Oligonucleotide Probes to Distinguish Intracellular Expression and Localization Patterns of Rab GDP-Dissociation Inhibitor Isoforms 6: Expression, Purification, and Biochemical Properties of Ypt/Rab GTPase-Activating Proteins of Gyp Family 7: Purification and Properties of Rab3 GDP/GTP Exchange Protein 8: Purification and Properties of Rab3 GTPase-Activating Protein 9: Rabphilin-3: A Target Molecule for Rab3 Small G Proteins 10: Doc2I+/- as Modulator of Ca2+-Dependent Exocytosis 11: Purification and Properties of a GTPase-Activating Protein for Yeast Rab GTPases 12: Purification and Characterization of Yeast Exocyst Complex 13: Expression and Properties of Rab4 and Its Effector Rabaptin-4 in Endocytic Recycling 14: Purification of EEA1 from Bovine Brain Cytosol Using Rab5 Affinity Chromatography and Activity Assays 15: Expression, Purification, and Characterization of Rab5 Effector Complex, Rabaptin-5/Rabex-5 16: Measurement of Rab5 Protein Kinase B/akt and Regulation of Ras-Activated Endocytosis 17: Expression, Purification, and Biochemical Properties of Rabkinesin-6 Domains and Their Interactions with Rab6A 18: Expression and Functional Analyses of Rab8 and Rablla in Exocytic Transport from trans-Golgi Network 19: Expression and Properties of Rab7 in Endosome Function 20: Expression, Purification, and Properties of Rab8 Function in Actin Cortical Skeleton Organization and Polarized Transport 21: Properties of Rab13 Interaction with Rod cGMP Phosphodiesterase I' Subunit 22: Subcellular Localization of Rab17 by Cryo-Immunogold Electron Microscopy in Epithelial Cells Grown on Polycarbonate Filters 23: Expression and Properties of Rab25 in Polarized Madin-Darby Canine Kidney Cells 24: Purification of TRAPP from Saccharomyces cerevisiae and Identification of Its Mammalian Counterpart Section II. ADP-Ribosylation Factor (ARF) GTPases 25: Structural and Functional Organization of ADP-Ribosylation Factor (ARF) Proteins 26: Expression and Properties of ADP-Ribosylation Factor (ARF6) in Endocytic Pathways 27: Expression and Analysis of ARNO and ARNO Mutants and Their Effects on ADP-Ribosylation Factor (ARF)-Mediated Actin Cytoskeletal Rearrangements 28: Expression, Purification, and Measurements of Activity of ARNO1, a Guanine Nucleotide Exchange Factor for ADP-Ribosylation Factor 1 (ARF1) 29: Expression, Purification, and Biochemical Properties of EFA6, a Sec7 Domain-Containing Guanine Exchange Factor for ADP-Ribosylation Factor 6 (ARF6) 30: Isolation and Properties of GRP1, an ADP-Ribosylation Factor (ARF)-Guanine Nucleotide Exchange Protein Regulated by Phosphatidylinositol 3, 4, 5-Trisphosphate 31: Functional Analysis of ADP-Ribosylation Factor (ARF) Guanine Nucleotide Exchange Factors Gea1p and Gea2p in Yeast 32: Isolation, Cloning, and Characterization of Brefeldin A-Inhibited Guanine Nucleotide-Exchange Protein for ADP-Ribosylation Factor 33: Expression, Purification, and Properties of ADP-Ribosylation Factor (ARF) GTPase Activating Protein-1 34: Expression, Analysis, and Properties of Yeast ADP-Ribosylation Factor (ARF) GTPase Activating Proteins (GAPs) Gcs1 and Glo3 35: Purification and Properties of ARD1, An ADP-Ribosylation Factor (ARF)-Related Protein with GTPase-Activating Domain 36: Purification and Characterization of GIT Family of ADP-Ribosylation Factor (ARF) GTPase-Activating Proteins 37: Assay and Purification of Phosphoinositide-Dependent ADP-Ribosylation Factor (ARF) GTPase Activating Proteins 38: Biological Properties and Measurement of Phospholipase D Activation by ADP-Ribosylation Factor (ARF) 39: Use of Aminoglycoside Antibiotics and Related Compounds to Study ADP-Ribosylation Factor (ARF) /Coatomer Function in Golgi Traffic 40: Adaptor Protein 1-Dependent Clathrin Coat Assembly on Synthetic Liposomes and Golgi Membranes 41: Receptor-Dependent Formation of COPI-Coated Vesicles from Chemically Defined Donor Liposomes 42: ADP-Ribosylation Factor (ARF) as Regulator of Spectrin Assembly at Golgi Complex 43: Purification, Properties, and Analysis of yARL3 44: Preparation and Assay of Recombinant ADP-Ribosylation Factor-Like Protein-1 (ARL1) Section III. Sar GTPases 45: Purification and Properties of Rat Liver Sec23aEURO"Sec24 Complex 46: Purification of Functional Sec13paEURO"Sec31p Complex, A Subunit of COPII Coat Section IV. Dynamin GTPases 47: Expression, Purification, and Functional Assays for Self-Association of Dynamin-1 48: Analysis of Phosphoinositide Binding by Pleckstrin Homology Domain from Dynamin 49: Mapping Dynamin Interdomain Interactions with Yeast Two-Hybrid and Glutathione S-Transferase Pulldown Experiments 50: Interactions of Dynamin and Amphiphysin with Liposomes 51: Activation of Dynamin GTPase Activity by Phosphoinositides and SH3 Domain-Containing Proteins Section V. Septin GTPases 52: Expression and Analysis of Properties of Septin CDCrel-1 in Exocytosis Author Index Subject Index
Series Title: Methods in enzymology (Print), v. 329.
Other Titles: GTPases involved in vesicular traffic
Responsibility: ed. by W.E. Balch, Channing J. Der, Alan Hall.
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