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Reporter gene assays : methods and protocols

Author: Robert Damoiseaux; Samuel Hasson
Publisher: New York, NY : Humana Press : Springer, [2018] ©2018
Series: Methods in molecular biology (Clifton, N.J.), v. 1755.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:

This volume explores the applications of reporter gene technology and the methodologies needed for their effective implementation.

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Genre/Form: Electronic books
Additional Physical Format: Print version:
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Robert Damoiseaux; Samuel Hasson
ISBN: 9781493977246 1493977245 9781493977222 1493977229
OCLC Number: 1031709027
Description: 1 online resource (xii, 234 pages) : illustrations (some color).
Contents: Genome-edited cell lines for high-throughput screening / Patricia Dranchak, John J. Moran, Ryan MacArthur, Camila Lopez-Anido, James Inglese, and John Svaren --
High-throughput firefly luciferase reporter assays / Ellen Siebring-van Olst and Victor W. van Beusechem --
Using the 2A protein coexpression system : multicistronic 2A vectors expressing gene(s) of interest and reporter proteins / Garry A. Luke and Martin D. Ryan --
Developing mammalian cellular clock models using firefly luciferase reporter / Chidambaram Ramanathan and Andrew C. Liu --
High-throughput screening method to identify alternative splicing regulators / Cheryl Stork and Sika Zheng --
High-throughput screening of a luciferase reporter of gene silencing on the inactive X chromosome / Alissa Keegan, Kathrin Plath, and Robert Damoiseaux --
Making it all work : functional genomics and reporter gene assays / Genevieve Welch, Robert Damoiseaux, and Loren Miraglia --
Reporter gene assays using transfectable functional genomics libraries / Genevieve Welch, Robert Damoiseaux, and Loren Miraglia --
Reporter gene assays using viral functional genomics libraries / Genevieve Welch, Robert Damoiseaux, and Loren Miraglia --
Using YFP as a reporter of gene expression in the green alga Chlamydomonas reinhardtii / Crysten E. Blaby-Haas, M. Dudley Page, and Sabeeha S. Merchant --
Gene expression analysis by arylsulfatase assays in the green alga Chlamydomonas reinhardtii / Ian K. Blaby and Crysten E. Blaby-Haas --
Endogenous locus reporter assays / Yaping Liu, Jeffrey Hermes, Jing Li, and Matthew Tudor --
High-content reporter assays / Erica Cook, Jeffrey Hermes, Jing Li, and Matthew Tudor --
Data mining and computational modeling of high-throughput screening datasets / Sean Ekins, Alex M. Clark, Krishna Dole, Kellan Gregory, Andrew M. Mcnutt, Anna Coulon Spektor, Charlie Weatherall, Nadia K. Litterman, and Barry A. Bunin --
Intravital imaging of human melanoma cells in the mouse ear skin by two-photon excitation microscopy / Nathan Y. Bentolila, Raymond L. Barnhill, Claire Lugassy, and Laurent A. Bentolila.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 1755.
Responsibility: edited by Robert Damoiseaux, Samuel Hasson.

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