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Retinal development : methods and protocols

Author: Shu-Zhen Wang
Publisher: New York : Humana Press : Springer, 2012.
Series: Methods in molecular biology (Clifton, N.J.), v. 884.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
In recent years, there have been major advances in the concepts and methodologies used in the study of retinal development at both cellular and molecular levels. These advanced methodologies have allowed and will continue to allow researchers to gain new insights into the molecular mechanisms underlying retinal development. In Retinal Development: Methods and Protocols, expert researchers in the field detail many of  Read more...
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Details

Genre/Form: Electronic books
Laboratory manuals
Additional Physical Format: Print version:
Retinal development.
New York : Humana Press : Springer, ©2012
(DLC) 2012938950
(OCoLC)773671429
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Shu-Zhen Wang
ISBN: 9781617798481 1617798487
OCLC Number: 795994038
Language Note: English.
Description: 1 online resource (xiv, 367 pages) : illustrations (some color).
Contents: Conditional control of gene expression in the mouse retina / Qian Ding and Lin Gan --
Generation of transgenic Xenopus using restriction enzyme-mediated integration / Mohammad Haeri and Barry E. Knox --
In vivo functional analysis of transcription factor : response element interaction using transgenic Xenopus laevis / Heithem M. El-Hodiri, Yi Pan, and Lisa E. Kelly --
Subretinal delivery and electroporation in pigmented and nonpigmented adult mouse eyes / John M. Nickerson, Penny Goodman, Micah A. Chrenek, Christiana J. Bernal, Lennart Berglin, T. Michael Redmond, and Jeffrey H. Boatright --
In ovo eye electroporation / Teri L. Belecky-Adams, Scott R. Hudson, and Sarika Tiwari --
Targeted microinjection of synthetic mRNAs to alter retina gene expression in Xenopus embryos / Sally A. Moody --
Testing retina fate commitment in Xenopus by blastomere deletion, transplantation, and explant culture / Sally A. Moody --
Application of Cre-loxP recombination for lineage tracing of adult zebrafish retinal stem cells / Rajesh Ramachandran, Aaron Reifler, Jin Wan, and Daniel Goldman --
Fate tracing of neurogenin2-expressing cells in the mouse retina using CreER(TM) : LacZ / Wenxin Ma and Shu-Zhen Wang --
In vitro explant culture and related protocols for the study of mouse retinal development / Kangxin Jin and Mengqing Xiang --
In vitro biochemical assays to monitor rhodopsin function / Joshua Sammons and Alecia K. Gross --
Transfection of primary embryonic chicken retinal cells using cationic lipid / Yi-Wen Hsieh and Xian-Jie Yang --
Production of high-titer RCAS retrovirus / Run-Tao Yan and Shu-Zhen Wang --
Chick retinal pigment epithelium transdifferentiation assay for proneural activities / Shu-Zhen Wang and Run-Tao Yan --
Studying the generation of regenerated retinal neuron from muller glia in the mouse eye. / Mike O. Karl and Thomas A. Reh --
Production and transplantation of retinal cells from human and mouse embryonic stem cells / Anna La Torre, Deepak A. Lamba, Anu Jayabalu, and Thomas A. Reh --
Light-induced photoreceptor degeneration in the Retina of the zebrafish / Scott Taylor, Jing Chen, Jing Luo, and Peter Hitchcock --
Microarray-based gene profiling analysis of muller glia-derived retinal stem cells in light-damaged retinas from adult zebrafish / Zhao Qin and Pamela A. Raymond --
Measuring rodent electroretinograms to assess retinal function / Molly E. Clark and Timothy W. Kraft --
Functional imaging of retinal photoreceptors and inner neurons using stimulus-evoked intrinsic optical signals / Xin-Cheng Yao and Yi-Chao Li --
Use of laser capture microdissection for analysis of retinal mRNA/miRNA Expression and DNA Methylation / Laszlo Hackler Jr, Tomohiro Masuda, Verity F. Oliver, Shannath L. Merbs, and Donald J. Zack --
Revealing looping organization of mammalian photoreceptor genes using chromosome conformation capture (3C) assays / Guang-Hua Peng and Shiming Chen --
Retinal transcriptome profiling by directional next-generation sequencing using 100 ng of total RNA / Matthew J. Brooks, Harsha Karur Rajasimha, and Anand Swaroop --
Exome sequencing : capture and sequencing of all human coding regions for disease gene discovery / Rinki Ratna Priya, Harsha Karur Rajasimha, Matthew J. Brooks, and Anand Swaroop --
Determination of posttranslational modifications of photoreceptor differentiation factor NRL : focus on SUMOylation / Jerome E. Roger, Jacob Nellissery, and Anand Swaroop.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 884.
Responsibility: edited by Shu-Zhen Wang.

Abstract:

This book details the protocols used for a wide range of experiments in retinal research, including manipulating gene expression in vivo, retinal regeneration and stem cell-based replacement, and  Read more...

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