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Retinoid protocols

Author: Christopher P F Redfern
Publisher: Totowa, N.J. : Humana Press, ©1998.
Series: Methods in molecular biology (Clifton, N.J.), v. 89.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
Christopher Redfern brings together in Retinoid Protocols a comprehensive collection of key biochemical and molecular methods for retinoid research. These easily reproducible techniques range from methods of handling and analyzing retinoids to advanced protocols for gene targeting, from binding protein function in transgenic animals to the study of nuclear retinoid receptors. In addition, the book makes accessible  Read more...
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Genre/Form: Electronic books
Laboratory manuals
Laboratory Manuals
Additional Physical Format: Print version:
Retinoid protocols.
Totowa, N.J. : Humana Press, ©1998
(DLC) 97044455
(OCoLC)37843809
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Christopher P F Redfern
ISBN: 9781592595730 1592595731
OCLC Number: 260242107
Language Note: English.
Description: 1 online resource (xvi, 434 pages) : illustrations.
Contents: Properties of retinoids : structure, handling, and preparation / Arun B. Barua, Harold C. Furr --
Quantitative analyses of naturally occurring retinoids / Joseph L. Napoli, Ronald L. Horst --
Detection and measurement of retinoic acid production by isolated tissues using retinoic acid-sensitive reporter cell lines / Michael Wagner --
Immunohistochemistry for CRBPs and CRABPs / Ulf Eriksson, Anne-Lee Gustafsson --
Whole-mount in situ hybridization of mouse embryos exposed to teratogenic levels of retinoic acid / Sally D. Lyn --
Reverse transcriptase-polymerase chain reaction (RT-PCR) for cellular retinoid-binding proteins / Andrew D. Loughney, Christopher P. Redfern --
Methods for producing recombinant human cellular retinaldehyde-binding protein / John W. Crabb, Yang Chen, Steve Goldflam, Karen West, James Kapron --
Expression and purification of CRABPs from E. coli / Joseph L. Napoli, Zuzana Sperkova, Marek Sperka, Paul D. Fiorella --
Purification and fluorescent titration of cellular retinol-binding protein / Giorgio Malpeli, Claudia Folli, Davide Cavazzini, Giovanni Sartori, Rodolfo Berni --
Fluorometric titration of the CRABPs / Andrew W. Norris, Ellen Li --
Expression and mutagenesis of retinol-binding protein / Manickavasagam Sundaram, Asipu Sivaprasadarao, John B. Findlay --
Interactions of retinol-binding protein with transthyretin and its receptor / Asipu Sivaprasadarao, Manickavasagam Sundaram, John B. Findlay --
Detection of conformational changes in cellular retinoid-binding proteins by limited proteolysis / Robert S. Jamison, Marcia E. Newcomer, David E. Ong --
Measurement of rates of dissociation of retinoids from the interphotoreceptor retinoid-binding protein / Noa Noy --
Use of antisense oligonucleotides to study the role of CRABPs in retinoic acid-induced gene expression / Paul Nugent, Robert M. Greene --
Preparation of polyclonal antibodies to retinoid receptors / Jacqueline A. Dyck, Vickie J. LaMorte --
Detection of RARs and RXRs in cells and tissues using specific ligand-binding assays and ligand-binding lmmunoprecipitation techniques / Elizabeth A. Allegretto --
Nonisotopic in situ hybridization for the detection of nuclear retinoid receptor transcripts in tissue sections / Xiaochun Xu, Reuben Lotan --
In situ hybridization with ³⁵S-labeled probes for retinoid receptors / Karen Niederreither, Pascal Dollé --
Isolation of retinoid receptors from manimalian cells / Ann K. Daly, Christopher P. Redfern --
Analysis of retinoid receptor phosphorylation / Ali Tahayato, Christophe Rachez, Pierre Formstecher, Philippe Lefebvre --
Photoaffinity labeling of RARs and mapping of labeled sites by an endoproteinase combination technique / Yuichi Hashimoto, Toru Sasaki --
PCR cloning of N-terminal RAR Isoforms and APL-associated PLZF-RAR[alpha] fusion proteins / Arthur Zelent --
RT-PCR in diagnosis and disease monitoring of acute promyelocytic leukemia (APL) / David Grimwade, Stephen Langabeer, Kathy Howe, Ellen Solomon --
A two-hybrid protein interaction system to identify factors that interact with retinoid and vitamin D receptors / Paul N. MacDonald --
Gel-shift analysis and identification of RXREs and RAREs by PCR-based selection / Myriam I. Baes, Peter E. Declercq --
Identification and cloning of RA-regulated genes by mRNA-differential display / Jay A. White, Martin Petkovich --
Gene targeting of retinoid receptors / David Lohnes.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 89.
Responsibility: edited by Christopher P.F. Redfern.
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Abstract:

Interest in retinoic acid, the main biologically active derivative of vi- min A or retinol, increased dramatically between 1989 and 1993, following the cloning of nuclear receptors or RARs reported  Read more...

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