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Rho GTPases : methods and protocols

Author: Francisco Rivero-Crespo
Publisher: New York, NY : Humana Press, ©2012.
Series: Methods in molecular biology (Clifton, N.J.), v. 827.; Springer protocols (Series)
Edition/Format:   Print book : EnglishView all editions and formats
Summary:

Rho GTPase: Methods and Protocols provides a historical overview of the field and an account of the phylogenetics of the Rho family, general biochemical methods, and functional assays that allow  Read more...

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Genre/Form: Laboratory manuals
Laboratory Manuals
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: Francisco Rivero-Crespo
ISBN: 9781617794414 1617794414 1617794422 9781617794421
OCLC Number: 744298127
Description: xiv, 407 pages : illustrations ; 26 cm.
Contents: Historical overview of Rho GTPases --
Rho GTPases: Deciphering the evolutionary history of a complex protein family --
Biochemical assays to characterize Rho GTPases --
How to analyze bacterial toxins targeting Rho GTPases --
Assessing ubiquitylation of Rho GTPases in mammalian cells --
Posttranslational lipid modification of Rho family small GTPases --
Analysis of the role of RhoGDI1 and isoprenylation in the degradation of RhoGTPases --
A quantitative fluorometric approach for measuring the interaction of RhoGDI with membranes and Rho GTPases --
Rho GTPases and cancer cell transendothelial migration --
Quantitative and robust assay to measure cell-cell contact assembly and maintenance --
Rho GTPase knockout induction in primary keratinocytes from adult mice --
Rho GTPase techniques in osteoclastogenesis --
Assessment of Rho GTPase signaling during neurite outgrowth --
Assessment of the role for Rho family GTPases in nadph oxidase activation --
Multiplex imaging of Rho family GTPase activities in living cells --
FRET-based imaging of rac and cdc42 activation during fc-receptor-mediated phagocytosis in macrophages --
High-throughput flow cytometry bead-based multiplex assay for identification of Rho GTPase inhibitors --
Functional analysis of Rho GTPase activation and inhibition in a bead-based miniaturized format --
Use of phage display for the identification of molecular sensors specific for activated Rho --
Identification of new interacting partners for atypical Rho GTPases: A silac-based approach --
Using zebrafish for studying Rho GTPases signaling in vivo --
Analysis of Rho GTPase function in axon pathfinding using caenorhabditis elegans --
Protocol for ex vivo incubation of drosophila primary post-embryonic haemocytes for real-time analyses --
Analysis of Rho GTPase activation in saccharomyces cerevisiae --
Assaying Rho GTPase-dependent processes in dictyostelium discoideum --
FRAP-based analysis of Rho GTPase-dependent polar exocytosis in pollen tubes.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 827.; Springer protocols (Series)
Responsibility: edited by Francisco Rivero.
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