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Ribonucleases. Volume 341, Part A

Author: Allen W Nicholson
Publisher: San Diego : Academic Press, ©2001.
Series: Methods in enzymology, volume 341, pt. A.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
This first of two volumes provides up-to-date, methods-related information on ribonuclease functions, assays, and applications. Chapter topics include the identification of, characterization of, and assays for secreted ribonucleases; viral ribonucleases, artificial and engineered ribonucleases, and ribozymes. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the  Read more...
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Genre/Form: Electronic books
Additional Physical Format: Print version:
Ribonucleases. Pt. A.
San Diego : Academic Press, ©2001
(OCoLC)48193898
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Allen W Nicholson
ISBN: 9780080496917 0080496911 0121822427 9780121822422
OCLC Number: 166230185
Description: 1 online resource (xxxvi, 734 pages) : illustrations.
Contents: Front Cover; Ribonucleases; Copyright Page; Table of Contents; Contributors to Volume 341; Preface; Volume in Series; Section I: Ribonuclease Classification and Review; Chapter 1. A Natural Classification of Ribonucleases; Chapter 2. The Ribonuclease T1 Family; Chapter 3. Ribonuclease T2; Chapter 4. The Ribonuclease P Family; Section II: Ribonuclease Assays; Chapter 5. Fast, Facile, Hypersensitive Assays for Ribonucleolytic Activity; Chapter 6. Activity Staining for Detection of Ribonucleases Using Dried Agarose Film Overlay Method after Isoelectric Focusing. Chapter 7. Gel Renaturation Assay for RibonucleasesChapter 8. Analysis of Ribonucleases Following Gel Electrophoresis; Chapter 9. Ribonuclease Assays Utilizing Toluidine Blue Indicator Plates, Methylene Blue, or Fluorescence Correlation Spectroscopy; Chapter 10. Ribonuclease Activities of Trypanosome RNA Editing Complex Directed to Cleave Specifically at a Chosen Site; Chapter 11. Ribonuclease YI*, RNA Structure Studies, and Variable Single-Strand Specificities of RNases; Section III: Secreted Ribonucleases. Chapter 12. Bovine Pancreatic Ribonuclease A: Oxidative and Conformational Folding StudiesChapter 13. Purification of Engineered Human Pancreatic Ribonuclease; Chapter 14. Degradation of Double-Stranded RNA by Mammalian Pancreatic-Type Ribonucleases; Chapter 15. Seminal Ribonuclease: Preparation of Natural and Recombinant Enzyme, Quaternary Isoforms, Isoenzymes, Monomeric Forms; Assay for Selective Cytotoxicity of the Enzyme; Chapter 16. Angiogenin; Chapter 17. Eosinophil-Derived Neurotoxin; Chapter 18. Eosinophil Cationic Protein; Chapter 19. Deciphering the Mechanism of RNase T1. Chapter 20. Mitogillin and Related Fungal RibotoxinsChapter 21. RNase U2 and a-Sarcin: A Study of Relationships; Chapter 22. Secretory Acid Ribonucleases from Tomato, Lycopersicon esculentum Mill.; Chapter 23. Leczyme; Section IV: Ribonucleases H; Chapter 24. Prokaryotic Type 2 RNases H; Chapter 25. RNase H1 of Saccharomyces cerevisiae: Methods and Nomenclature; Chapter 26. Ribonucleases H of the Budding Yeast, Saccharomyces cerevisiae; Chapter 27. Human RNases H; Chapter 28. Assays for Retroviral RNase H; Section V: Synthetic Ribonucleases. Chapter 29. Sequence-Selective Artificial RibonucleasesChapter 30. RNA Cleavage by 1,4-Diazabicyclo[2.2.2]- octane-Imidazole Conjugates; Chapter 31. Preparation and Use of ZFY-6 Zinc Finger Ribonuclease; Section VI: Ribonucleolytic Nucleic Acids; Chapter 32. RNA Cleavage by the 10-23 DNA Enzyme; Chapter 33. Leadzyme; Chapter 34. Hammerhead Ribozyme Structure and Function in Plant RNA Replication; Chapter 35. Kinetic Analysis of Bimolecular Hepatitis delta Ribozyme; Chapter 36. Catalytic and Structural Assays for the Hairpin Ribozyme; Chapter 37. Intracellular Applications of Ribozymes.
Series Title: Methods in enzymology, volume 341, pt. A.
Responsibility: edited by Allen W. Nicholson.

Abstract:

This first of two volumes provides up-to-date, methods-related information on ribonuclease functions, assays, and applications. Chapter topics include the identification of, characterization of, and  Read more...

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PRAISE FOR THE SERIES"The Methods in Enzymology series represents the gold-standard."--NEUROSCIENCE"Incomparably useful." --ANALYTICAL BIOCHEMISTRY"It is a true 'methods' series, including almost Read more...

 
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