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| Genre/Form: | Laboratory Manuals |
|---|---|
| Document Type: | Book |
| All Authors / Contributors: |
Alexander Serganov |
| ISBN: | 9781934115886 1934115886 9781597455589 159745558X |
| OCLC Number: | 316002776 |
| Description: | xi, 366 p. . ill. ; 27 cm. |
| Contents: | Predicting riboswitch regulation on a genomic scale / J.E. Barrick -- Enzymatic ligation strategies for the preparation of purine riboswitches with site-specific chemical modifications / R. Rieder, C. Hobartner and R. Micura -- Application of fluorescent measurements for characterization of riboswitch-ligand interactions / B. Heppell ... [et al.] -- Transcriptional approaches to riboswitch studies / A. Mironov, V. Epshtein and E. Nudler -- Kinetics of riboswitch regulation studied by in vitro transcription / J.K. Wickiser -- Molecular basis of RNA-mediated gene regulation on the adenine riboswitch by single-molecule approaches / J.F. Lemay ... [et al.] -- Methods for analysis of ligand-induced RNA conformational changes / C.A. Brautigam, C.A. Wakeman and W.C. Winkler -- Monitoring RNA-ligand interactions using isothermal titration calorimetry / S.D. Gilbert and R.T. Batey -- Preparation and crystallization of riboswitch-ligand complexes / O. Pikovskaya ... [et al.] -- Crystallization of the glmS ribozyme-riboswitch / D.J. Klein and A.R. Ferre-D'Amare -- Riboswitch conformations revealed by small-angle X-ray scattering / J. Lipfert, D. Herschlag and S. Doniach -- Time-resolved NMR spectroscopy: ligand-induced refolding of riboswitches / J. Buck ... [et al.] -- Analysis of the RNA backbone: structural analysis of riboswitches by in-line probing and selective 2'-hydroxyl acylation and primer extension / C.A. Wakeman and W.C. Winkler -- Identification of metabolite-riboswitch interactions using nucleotide analog interference mapping and suppression / J.K. Soukup and G.A. Soukup -- RNA-dependent RNA switches in bacteria / T.M. Henkin -- Probing mRNA structure and sRNA-mRNA interactions in bacteria using enzymes and lead(II) / C. Chevalier ... [et al.] -- Structural probing of RNA thermosensors / C. Chiaruttini, F. Allem and M. Springer -- Ribosomal initiation complexes probed by toeprinting and effect of trans-acting translational regulators in bacteria / P. Fechter ... [et al.] -- Isolation and characterization of the heat shock RNA 1 / I. Shamovsky and E. Nudler -- Analysis of tRNA-directed transcription antitermination in the T box system in vivo / T.M. Henkin -- In vitro selection of conformational probes for riboswitches / G. Mayer and M. Famulok -- A green fluorescent protein (GFP)-based plasmid system to study post-transcriptional control of gene expression in vivo / J.H. Urban and J. Vogel -- High-throughput screens to discover synthetic riboswitches / S.A. Lynch, S. Topp and J.P. Gallivan -- A mammalian cell-based assay for screening inhibitors of RNA cleavage / L. Yen, B.R. Stockwell and R.C. Mulligan -- In vitro selection of glmS ribozymes / K.H. Link and R.R. Breaker. |
| Series Title: | Methods in molecular biology (Clifton, N.J.), v. 540.; Springer protocols. |
| Responsibility: | edited by Alexander Serganov. |
Reviews
Publisher Synopsis
From the reviews: "Riboswitches are among the latest additions to the growing field of RNA-based gene expression control systems found in both prokaryotic and eukaryotic cells. ! useful in various aspects of work on riboswitches and other regulatory RNAs. ! It also refers to pertinent and up-to-date literature. ! The reviewed book can be strongly recommended to RNA researchers with different levels of expertise, as well as to those who learn or teach in this field." (Joanna Ciesla, Acta Biochimica Polonica, Vol. 56 (3), 2009) "This book is a good collection of 'Methods and Protocols' for studying ! riboswitches as well as some other RNAs involved in gene expression. ! Readers are introduced to a broad range of RNA methodologies from basic to applied in this book. I ! recommend the book for researchers who work in the area of riboswitches, but also for other scientists who are involved in RNA-relevant studies." (Atsushi Ogawa, ChemBioChem, Vol. 10, 2009) Read more...
