skip to content
Riboswitches : methods and protocols Preview this item
ClosePreview this item
Checking...

Riboswitches : methods and protocols

Author: Alexander Serganov
Publisher: New York : Humana, ©2009.
Series: Methods in molecular biology (Clifton, N.J.), v. 540.; Springer protocols (Series)
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
The revolutionary discoveries of RNA interference and bacterial regulatory RNAs led to further breakthroughs such as the identification of riboswitches and related RNA sensors, mRNA regions capable of alternating their conformations in response to the presence of cellular metabolites and other physical or chemical cues. In Riboswitches: Methods and Protocols, expert researchers provide comprehensive and up-to-date  Read more...
Rating:

(not yet rated) 0 with reviews - Be the first.

Subjects
More like this

Find a copy in the library

&AllPage.SpinnerRetrieving; Finding libraries that hold this item...

Details

Genre/Form: Electronic books
Laboratory Manuals
Additional Physical Format: Print version:
Riboswitches.
New York : Humana, ©2009
(DLC) 2008944038
(OCoLC)316002776
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Alexander Serganov
ISBN: 9781597455589 159745558X
OCLC Number: 320959049
Language Note: English.
Description: 1 online resource (xi, 366 pages) : illustrations.
Contents: Predicting riboswitch regulation on a genomic scale / Jeffrey E. Barrick --
Enzymatic ligation strategies for the preparation of purine riboswitches with site-specific chemical modification / Renate Rieder, Claudia Höbartner and Ronald Micura --
Application of fluorescent measurements for characterization of riboswitch-ligand interactions / Benoit Heppell [and others] --
Transcriptional approaches to riboswitch studies / Alexander Mironov, Vitaly Epshtein and Evgeny Nudler --
Kinetics of riboswitch regulation studied by in vitro transciption / J. Kenneth Wickiser --
Molecular basis of RNA-mediated gene regulation on the adenine riboswitch by single-molecule approaches / Jean-Franc̜ois Lemay [and others] --
Methods for analysis of ligand-induced RNA confromational changes / Chad A. Brautigam, Catherine A. Wakeman and Wade C. Winkler --
Preparation and crystallization of riboswitch-ligand complexes / Olga Pikovskaya [and others] --
Crystallization of the glmS ribozyme-riboswitch / Daniel J. Klein and Adrian R. Ferré-D'Amaré --
Riboswitch conformations revealed by small-angle X-ray scattering / Jan Lipfert, Daniel Herschlag and Sebastian Doniach --
Time-resolved NMR spectroscopy : ligand-induced refolding of riboswitches / Janina Buck [and others] --
Analysis of the RNA backbone : structural Analysis of riboswitches by in-line probing and selective 2'-hydroxyl acylation and primer extension / Catherine A. Wakeman and Wade C. Winkler --
Identification of metabolite-riboswitch interactions using nucleotide analog interference mapping and suppression / Juliane K. Soukup and Garrett A. Soukup --
RNA-dependent RNA switches in bacteria / Tina M. Henkin --
Probing mRNA structure and sRNA-mRNA interactions in bacteria using enzymes and lead(II) / Clément Chevalier [and others] --
Structural probing of RNA thermosensors / Claude Chiaruttini, Frédéric Allem and Mathias Springer --
Ribosomal initiation complexes probed by toeprinting and effect of trans-acting translational regulators in bacteria / Pierre Fechter [and others] --
Isolation and characterization of the heat shock RNA 1 / Ilya Shamovsky and Evgeny Nudler --
Analysis of tRNA-directed transcription antitermination in the T box system in vivo / Tina M. Henkin --
In vitro selection of conformational probes for riboswitches / Günter Mayer and Michael Famulok --
A green fluorescent protein (GFP)-based plasmid system to study post-transcriptional control of gene expression in vivo / Johannes H. Urban and Jörg Vogel --
High-throughput screens to Discover synthetic riboswitches / Sean A. Lynch, Shana Topp and Justin P. Gallivan --
A mammalian cell-based assay for screening inhibitors of RNA cleavage / Laising Yen, Brent R. Stockwell and Richard C. Mulligan --
In vitro selection of glmS ribozymes / Kristian H. Link and Ronald R. Breaker.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 540.; Springer protocols (Series)
Responsibility: edited by Alexander Serganov.

Abstract:

This book provides comprehensive, up-to-date coverage of many methods used to study riboswitches and other RNAs involved in gene expression control. It focuses on mRNAs responding to small organic  Read more...

Reviews

Editorial reviews

Publisher Synopsis

From the reviews:"Riboswitches are among the latest additions to the growing field of RNA-based gene expression control systems found in both prokaryotic and eukaryotic cells. ... useful in various Read more...

 
User-contributed reviews
Retrieving GoodReads reviews...
Retrieving DOGObooks reviews...

Tags

Be the first.
Confirm this request

You may have already requested this item. Please select Ok if you would like to proceed with this request anyway.

Linked Data


Primary Entity

<http://www.worldcat.org/oclc/320959049> # Riboswitches : methods and protocols
    a schema:Book, schema:MediaObject, schema:CreativeWork ;
    library:oclcnum "320959049" ;
    library:placeOfPublication <http://id.loc.gov/vocabulary/countries/nyu> ;
    library:placeOfPublication <http://dbpedia.org/resource/New_York_City> ; # New York
    schema:about <http://id.worldcat.org/fast/1086244> ; # RNA
    schema:about <http://experiment.worldcat.org/entity/work/data/895353802#Topic/gene_expression_regulation_physiology> ; # Gene Expression Regulation--physiology
    schema:about <http://experiment.worldcat.org/entity/work/data/895353802#Topic/clinical_laboratory_techniques> ; # Clinical Laboratory Techniques
    schema:about <http://id.worldcat.org/fast/1017394> ; # Messenger RNA
    schema:about <http://id.worldcat.org/fast/998471> ; # Ligands (Biochemistry)
    schema:about <http://dewey.info/class/572/e23/> ;
    schema:about <http://id.worldcat.org/fast/1086260> ; # RNA-protein interactions
    schema:about <http://experiment.worldcat.org/entity/work/data/895353802#Topic/rna_chemistry> ; # RNA--chemistry
    schema:bookFormat schema:EBook ;
    schema:contributor <http://viaf.org/viaf/88424333> ; # Alexander Serganov
    schema:copyrightYear "2009" ;
    schema:datePublished "2009" ;
    schema:description "The revolutionary discoveries of RNA interference and bacterial regulatory RNAs led to further breakthroughs such as the identification of riboswitches and related RNA sensors, mRNA regions capable of alternating their conformations in response to the presence of cellular metabolites and other physical or chemical cues. In Riboswitches: Methods and Protocols, expert researchers provide comprehensive and up-to-date coverage of various methods used to study riboswitches and other RNAs involved in gene expression control. Examining biochemical and modern biophysical techniques, the volume focuses on mRNAs responding to small organic molecules but expands the definition of a riboswitch to incorporate classes of RNA that undergo conformational transitions in response to other stimuli in order to control the expression of genes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Riboswitches: Methods and Protocols promises to inspire both novices and experts working in a wide range of contemporary biological areas who wish to further develop their RNA methodology."@en ;
    schema:description "Predicting riboswitch regulation on a genomic scale / Jeffrey E. Barrick -- Enzymatic ligation strategies for the preparation of purine riboswitches with site-specific chemical modification / Renate Rieder, Claudia Höbartner and Ronald Micura -- Application of fluorescent measurements for characterization of riboswitch-ligand interactions / Benoit Heppell [and others] -- Transcriptional approaches to riboswitch studies / Alexander Mironov, Vitaly Epshtein and Evgeny Nudler -- Kinetics of riboswitch regulation studied by in vitro transciption / J. Kenneth Wickiser -- Molecular basis of RNA-mediated gene regulation on the adenine riboswitch by single-molecule approaches / Jean-Franc̜ois Lemay [and others] -- Methods for analysis of ligand-induced RNA confromational changes / Chad A. Brautigam, Catherine A. Wakeman and Wade C. Winkler -- Preparation and crystallization of riboswitch-ligand complexes / Olga Pikovskaya [and others] -- Crystallization of the glmS ribozyme-riboswitch / Daniel J. Klein and Adrian R. Ferré-D'Amaré -- Riboswitch conformations revealed by small-angle X-ray scattering / Jan Lipfert, Daniel Herschlag and Sebastian Doniach -- Time-resolved NMR spectroscopy : ligand-induced refolding of riboswitches / Janina Buck [and others] -- Analysis of the RNA backbone : structural Analysis of riboswitches by in-line probing and selective 2'-hydroxyl acylation and primer extension / Catherine A. Wakeman and Wade C. Winkler -- Identification of metabolite-riboswitch interactions using nucleotide analog interference mapping and suppression / Juliane K. Soukup and Garrett A. Soukup -- RNA-dependent RNA switches in bacteria / Tina M. Henkin -- Probing mRNA structure and sRNA-mRNA interactions in bacteria using enzymes and lead(II) / Clément Chevalier [and others] -- Structural probing of RNA thermosensors / Claude Chiaruttini, Frédéric Allem and Mathias Springer -- Ribosomal initiation complexes probed by toeprinting and effect of trans-acting translational regulators in bacteria / Pierre Fechter [and others] -- Isolation and characterization of the heat shock RNA 1 / Ilya Shamovsky and Evgeny Nudler -- Analysis of tRNA-directed transcription antitermination in the T box system in vivo / Tina M. Henkin -- In vitro selection of conformational probes for riboswitches / Günter Mayer and Michael Famulok -- A green fluorescent protein (GFP)-based plasmid system to study post-transcriptional control of gene expression in vivo / Johannes H. Urban and Jörg Vogel -- High-throughput screens to Discover synthetic riboswitches / Sean A. Lynch, Shana Topp and Justin P. Gallivan -- A mammalian cell-based assay for screening inhibitors of RNA cleavage / Laising Yen, Brent R. Stockwell and Richard C. Mulligan -- In vitro selection of glmS ribozymes / Kristian H. Link and Ronald R. Breaker."@en ;
    schema:exampleOfWork <http://worldcat.org/entity/work/id/895353802> ;
    schema:genre "Laboratory Manuals"@en ;
    schema:genre "Electronic books"@en ;
    schema:inLanguage "en" ;
    schema:isPartOf <http://worldcat.org/issn/1064-3745> ; # Methods in molecular biology,
    schema:isPartOf <http://experiment.worldcat.org/entity/work/data/895353802#Series/springer_protocols> ; # Springer protocols
    schema:isPartOf <http://experiment.worldcat.org/entity/work/data/895353802#Series/methods_in_molecular_biology_clifton_n_j> ; # Methods in molecular biology (Clifton, N.J.) ;
    schema:isPartOf <http://experiment.worldcat.org/entity/work/data/895353802#Series/springer_protocols_series> ; # Springer protocols (Series)
    schema:isSimilarTo <http://www.worldcat.org/oclc/316002776> ;
    schema:name "Riboswitches : methods and protocols"@en ;
    schema:productID "320959049" ;
    schema:publication <http://www.worldcat.org/title/-/oclc/320959049#PublicationEvent/new_york_humana_2009> ;
    schema:publisher <http://experiment.worldcat.org/entity/work/data/895353802#Agent/humana> ; # Humana
    schema:url <http://dx.doi.org/10.1007/978-1-59745-558-9> ;
    schema:url <https://link.springer.com/openurl?genre=book&isbn=978-1-934115-88-6> ;
    schema:url <http://link.springer.com/openurl?genre=book&isbn=978-1-59745-558-9> ;
    schema:url <http://www.springerlink.com/content/p84737/?sortorder=asc&p_o=20> ;
    schema:url <http://www.springerprotocols.com/doiresolver?genre=book&pid=10.1007/978-1-59745-558-9> ;
    schema:url <http://www.springerprotocols.com/BookToc/doi/10.1007/978-1-59745-558-9> ;
    schema:url <http://link.springer.com/openurl?genre=book&isbn=978-1-934115-88-6> ;
    schema:url <https://0-link-springer-com.pugwash.lib.warwick.ac.uk/book/10.1007/978-1-59745-558-9> ;
    schema:url <http://link.springer.com/10.1007/978-1-59745-558-9> ;
    schema:url <http://link.springer.com/book/10.1007/978-1-59745-558-9> ;
    schema:workExample <http://worldcat.org/isbn/9781597455589> ;
    schema:workExample <http://dx.doi.org/10.1007/978-1-59745-558-9> ;
    umbel:isLike <http://d-nb.info/990588394> ;
    wdrs:describedby <http://www.worldcat.org/title/-/oclc/320959049> ;
    .


Related Entities

<http://dbpedia.org/resource/New_York_City> # New York
    a schema:Place ;
    schema:name "New York" ;
    .

<http://experiment.worldcat.org/entity/work/data/895353802#Series/methods_in_molecular_biology_clifton_n_j> # Methods in molecular biology (Clifton, N.J.) ;
    a bgn:PublicationSeries ;
    schema:hasPart <http://www.worldcat.org/oclc/320959049> ; # Riboswitches : methods and protocols
    schema:name "Methods in molecular biology (Clifton, N.J.) ;" ;
    .

<http://experiment.worldcat.org/entity/work/data/895353802#Series/springer_protocols> # Springer protocols
    a bgn:PublicationSeries ;
    schema:hasPart <http://www.worldcat.org/oclc/320959049> ; # Riboswitches : methods and protocols
    schema:name "Springer protocols" ;
    .

<http://experiment.worldcat.org/entity/work/data/895353802#Series/springer_protocols_series> # Springer protocols (Series)
    a bgn:PublicationSeries ;
    schema:hasPart <http://www.worldcat.org/oclc/320959049> ; # Riboswitches : methods and protocols
    schema:name "Springer protocols (Series)" ;
    .

<http://experiment.worldcat.org/entity/work/data/895353802#Topic/clinical_laboratory_techniques> # Clinical Laboratory Techniques
    a schema:Intangible ;
    schema:name "Clinical Laboratory Techniques"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/895353802#Topic/gene_expression_regulation_physiology> # Gene Expression Regulation--physiology
    a schema:Intangible ;
    schema:name "Gene Expression Regulation--physiology"@en ;
    .

<http://id.worldcat.org/fast/1017394> # Messenger RNA
    a schema:Intangible ;
    schema:name "Messenger RNA"@en ;
    .

<http://id.worldcat.org/fast/1086244> # RNA
    a schema:Intangible ;
    schema:name "RNA"@en ;
    .

<http://id.worldcat.org/fast/1086260> # RNA-protein interactions
    a schema:Intangible ;
    schema:name "RNA-protein interactions"@en ;
    .

<http://id.worldcat.org/fast/998471> # Ligands (Biochemistry)
    a schema:Intangible ;
    schema:name "Ligands (Biochemistry)"@en ;
    .

<http://viaf.org/viaf/88424333> # Alexander Serganov
    a schema:Person ;
    schema:familyName "Serganov" ;
    schema:givenName "Alexander" ;
    schema:name "Alexander Serganov" ;
    .

<http://worldcat.org/isbn/9781597455589>
    a schema:ProductModel ;
    schema:isbn "159745558X" ;
    schema:isbn "9781597455589" ;
    .

<http://worldcat.org/issn/1064-3745> # Methods in molecular biology,
    a bgn:PublicationSeries ;
    schema:hasPart <http://www.worldcat.org/oclc/320959049> ; # Riboswitches : methods and protocols
    schema:issn "1064-3745" ;
    schema:name "Methods in molecular biology," ;
    .

<http://www.worldcat.org/oclc/316002776>
    a schema:CreativeWork ;
    rdfs:label "Riboswitches." ;
    schema:description "Print version:" ;
    schema:isSimilarTo <http://www.worldcat.org/oclc/320959049> ; # Riboswitches : methods and protocols
    .


Content-negotiable representations

Close Window

Please sign in to WorldCat 

Don't have an account? You can easily create a free account.