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Ribozyme protocols

Author: Philip C Turner
Publisher: Totowa, N.J. : Humana Press, ©1997.
Series: Methods in molecular biology (Clifton, N.J.), v. 74.
Edition/Format:   Print book : EnglishView all editions and formats
Summary:

The purpose of Ribozyme Protocols is to provide a helpful compilation of protocols that will be of use-^not only to those with some experience of ribozymes-^but also to those wishing to use ribozymes  Read more...

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Genre/Form: Aufsatzsammlung
Laboratory manuals
Additional Physical Format: Online version:
Ribozyme protocols.
Totowa, N.J. : Humana Press, ©1997
(OCoLC)644113867
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: Philip C Turner
ISBN: 0896033899 9780896033894
OCLC Number: 36557208
Description: xiv, 492 pages : illustrations ; 24 cm.
Contents: Ribozymes : an introduction / Helen A. James, Philip C. Turner --
Computer-aided calculation of the local folding potential of target RNA and its use for ribozyme design / Georg Sczakiel, Martin Tabler --
Computational approaches to the identification of ribozyme target sites / William James, Elizabeth Cowe --
Computer analysis of the conservation and uniqueness of ribozyme-targeted HIV sequences / Mary Beth Young, Arnold Hampel --
Selection of accessible sites for ribozymes on large RNA transcripts / Bruce L. Frank, John Goodchild --
Selection of efficient ribozyme cleavage sites in target RNAs / Andre Lieber, Mikkel Rohde, Michael Strauss --
Chemical synthesis, analysis, and purification of ribozymes / Ravi Vinayak --
A practical method for the production of RNA and ribozymes / Francine E. Wincott, Nassim Usman --
Preparation of templates for production of ribozymes and substrates / Rajesh K. Gaur, Guido Krupp --
Enzymatic synthesis and characterization of unmodified ribozymes and substrates / Guido Krupp --
T7 transcript length determination using enzymatic RNA sequencing / Andrew Siwkowski --
Chemical and enzymatic approaches to construct modified RNAs / Rajesh K. Gaur, Guido Krupp --
Applications of modified transcripts / Rajesh K. Gaur, Frank Conrad, Guido Krupp --
Cloning strategies for catalytic antisense RNAs / Martin Tabler, Mina Tsagris --
PCR-based construction of long hammerhead ribozymes / Martin Zillmann, Gregory Robinson --
Design and production of asymmetric hammerhead ribozymes / Martin Tabler, Georg Sczakiel --
Minimized hammerhead ribozymes / Maxine J. McCall, Philip Hendry, Trevor J. Lockett --
Design of hairpin ribozymes for in vitro and cellular applications / Qiao Yu, John M. Burke --
Design of the hairpin ribozyme for targeting specific RNA sequences / Arnold Hampel, Mary Beth Young, Scott Galasinski, Andrew Siwkowski --
Design and preparation of sequence-specific RNase P ribozymes / Denis Drainas, Guido Krupp --
Theoretical considerations in measuring reaction parameters / Timothy S. McConnell --
Experimental approaches for measuring reaction parameters / Timothy S. McConnell --
Determination of catalytic parameters for hairpin ribozymes / Mary Beth Young, Andrew Siwkowski, Arnold Hampel --
Characterizing ribozyme cleavage reactions / Philip Hendry, Maxine J. McCall, Trevor J. Lockett --
Defining optimum reaction conditions for hammerhead ribozymes / Philip Hendry, Maxine J. McCall, Trevor J. Lockett --
Using fluorescence resonance energy transfer to investigate hammerhead ribozyme kinetics / Thomas A. Perkins, John Goodchild --
Design of hybridizing arms in hammerhead ribozymes / Philip Hendry, Maxine J. McCall, Trevor J. Lockett --
Optimization of hammerhead flanking sequences using oligonucleotide facilitators / John Goodchild --
Enhancement of ribozyme function by RNA binding proteins / Nan Sook Lee, Edouard Bertrand, John J. Rossi --
Selection of fast-hybridizing complementary RNA species in vitro / Ralf Kronenwett, Georg Sczakiel --
In vitro selection of hairpin ribozymes / Bruno Sargueil, John M. Burke --
The detection of hammerhead ribozyme cleavage by RT-PCR methods / Rhonda Perriman --
Detection of ribozyme cleavage products using reverse ligation-mediated PCR (RL-PCR) / Edouard Bertrand, Micheline Fromont-Racine, Raymond Pictet, Thierry Grange --
Quantitation of ribozyme target abundance by QCPCR / Amber A. Beaudry, James A. McSwiggen --
Trans-splicing reactions by ribozymes / Joshua T. Jones, Seong-Wook Lee, Bruce A. Sullenger --
Ligation of RNA molecules by the hairpin ribozyme / Alfredo Bertal-Herranz, John M. Burke --
Mutagenesis and modeling of the hairpin ribozyme family / Andrew Siwkowski, Mary Beth DeYoung, Pamela Anderson, Arnold Hampel --
Preparation of homogeneous ribozyme RNA for crystallization / Jennifer A. Doudna --
Establishing suitability of RNA preparations for crystallization : determination of polydispersity / Adrian R. Ferré-d'Amaré, Jennifer A. Doudna --
A sparse matrix approach to crystallizing ribozymes and RNA motifs / Jamie H. Cate, Jennifer A. Doudna --
Crystallographic analyses of chemically synthesized modified hammerhead RNA sequences as a general approach toward understanding ribozyme structure and function / William G. Scott --
tRNA delivery systems for ribozymes / Rhonda Perriman, Rob de Feyter --
Expressing ribozymes in plants / Rob de Feyter, Judith Gaudron --
Using microinjection of Xenopus oocytes to express and optimize ribozymes in vivo / Philip C. Turner --
Exogenous cellular delivery of ribozymes and ribozyme encoding DNAs / Daniela Castanotto, Edouard Bertrand, John Rossi --
Optimization of lipid-mediated ribozyme delivery to cells in culture / Suzy A. Brown, Thale C. Jarvis --
Retroviral delivery and anti-HIV testing of hammerhead ribozymes / Laurence Cagnon, John Rossi --
Hairpin ribozyme gene therapy for AIDS / Elizabeth A. Duarte, Mark C. Leavitt, Osamu Yamada, Mang Yu --
Clinical aspects of ribozymes as therapeutics in gene therapy / David Looney, Mang Yu.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 74.
Responsibility: edited by Philip C. Turner.

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