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RNA abundance analysis : methods and protocols

Author: Hailing Jin; Walter Gassmann
Publisher: New York : Humana Press : Springer, ©2012.
Series: Methods in molecular biology (Clifton, N.J.), v. 883.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Database:WorldCat
Summary:
RNA abundance analysis is one of the most important approaches for gene expression studies in the field of molecular biology. In RNA Abundance Analysis: Methods and Protocols, expert researchers cover a wide range of techniques on RNA extraction, detection, quantification, visualization, and genome-wide profiling, from conventional methods to state-of-the-art high throughput approaches. This volume includes detailed  Read more...
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Genre/Form: Electronic books
Laboratory manuals
Additional Physical Format: Print version:
RNA abundance analysis.
New York : Humana Press ; Springer, ©2012
(OCoLC)773666130
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Hailing Jin; Walter Gassmann
ISBN: 9781617798399 1617798398
OCLC Number: 794007806
Description: 1 online resource (xiii, 231 pages) : illustrations.
Contents: SuperSAGE : powerful serial analysis of gene expression / Hideo Matsumura [and others] --
Northern blot analysis for expression profiling of mRNAs and small RNAs / Ankur R. Bhardwaj [and others] --
Construction of RNA-seq libraries from large and microscopic tissues for the illumina sequencing platform / Hagop S. Atamian and Isgouhi Kaloshian --
Strand-specific RNA-seq applied to malaria samples / Nadia Ponts, Duk-Won D. Chung, and Karine G. Le Roch --
RNA in situ hybridization in Arabidopsis / Miin-Feng Wu and Doris Wagner --
Laser microdissection of cells and isolation of high-quality RNA after cryosectioning / Marta Barcala, Carmen Fenoll, and Carolina Escobar --
Detection and quantification of alternative splicing variants using RNA-seq / Douglas W. Bryant Jr, Henry D. Priest, and Todd C. Mockler --
Separating and analyzing sulfur-containing RNAs with Organomercury Gels / Elisa Biondi and Donald H. Burke --
RNAse mapping and quantitation of RNA isoforms / Lakshminarayan K. Venkatesh, Olufemi Fasina, and David J. Pintel --
Detection and quantification of viral and satellite RNAs in plant hosts / Sun-Jung Kwon, Jang-Kyun Seo, and A.L.N. Rao --
In situ detection of mature miRNAs in plants using LNA-modified DNA probes / Xiaozhen Yao, Hai Huang, and Lin Xu --
Small RNA isolation and library construction for expression profiling of small RNAs from neurospora and fusarium using illumina high-throughput deep sequencing / Gyungsoon Park and Katherine A. Borkovich --
Isolation and profiling of protein-associated small RNAs / Hongwei Zhao [and others] --
New virus discovery by deep sequencing of small RNAs / Kashmir Singh, Ravneet Kaur, and Wenping Qiu --
Global assembly of expressed sequence tags / Foo Cheung --
Computational analysis of RNA-seq / Scott A. Givan, Christopher A. Bottoms, and William G. Spollen --
Identification of microRNAs and natural antisense transcript-originated endogenous siRNAs from small-RNA deep sequencing data / Weixiong Zhang [and others].
Series Title: Methods in molecular biology (Clifton, N.J.), v. 883.
Responsibility: edited by Hailing Jin, Walter Gassmann.
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Abstract:

This book examines a wide range of techniques on RNA extraction, detection, quantification, visualization, and genome-wide profiling, from conventional methods to state-of-the-art high throughput  Read more...

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