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RNA interference

Author: David R Engelke; John J Rossi
Publisher: San Diego : Elsevier Academic Press, ©2005.
Series: Methods in enzymology, v. 392.
Edition/Format:   Print book : EnglishView all editions and formats
Summary:

Covers RNAi in non-vertebrates (plants, C elegans, drosophila, and S pombe), and Mammalian systems (human and non-human cells). This volume discusses extensive methodology related to delivery  Read more...

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Genre/Form: Handbooks and manuals
Fulltext
Handbooks
Internet Resources
Handbooks, manuals, etc
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: David R Engelke; John J Rossi
ISBN: 0121827976 9780121827977
OCLC Number: 57676191
Description: xxxvii, 453, [13] pages of plates : illustrations (some color) ; 24 cm.
Contents: Transgene-induced RNA interference as a tool for plant functional genomics / Karen McGinnis, Vicki Chandler, Karen Cone, Heidi Kaeppler, Shawn Kaeppler, Arthur Kerschen, Craig Pikaard, Eric Richards, Lyudmila Sidorenko, Todd Smith, Nathan Springer and Tuya Wulan --
Constructs and methods for hairpin RNA-mediated gene silencing in plants / Chris A. Helliwell and Peter M. Waterhouse --
RNA interference in Caenorhabditis elegans / Juan Wang and Maureen M. Waterhouse --
High-throughput RNA interference screens in Drosophila tissue culture cells / Susan Armknecht, Michael Boutros, Amy Kiger, Kent Nybakken, Bernard Mathey-Prevot and Norbert Perrimon --
Mechanistic insights aid computational short interfering RNA design / Queta Boese, Devin Leake, Angela Reynolds, Steven Read, Stephen A. Scaringe, William S. Marshall and Anastasia Khvorova --
Novel methods for expressing RNA interference in human cells / Masayuki Sano, Yoshio Kato, Hideo Akashi, Makoto Miyagishi and Kazunari Taira --
Delivery of small interfering RNA to mammalian cells in culture by using cationic lipid/polymer-based transfection reagents / Robert M. Brazas and James E. Hagstrom --
Subcellular distribution of small interfering RNA: directed delivery through RNA polymerase III expresson cassettes and localization by In Situ hybridization / Cynthia P. Paul --
Viral delivery of recombinant short hairpin RNAs / Beverly L. Davidson and Scott Q. Harper --
Targeting cellular genes with PCR cassettes expressing short interfering RNAs / Daniela Castanotto and Lisa Scherer --
Use of short hairpin RNA expression vectors to study mammalian neural development / Jenn-Yah Yu, Tsu-Wei Wang, Anne B. Vojtek, Jack M. Parent and David L. Turner --
Analysis of T-cell development by using short interfacing RNA to knock down protein expression / Gabriela Hernandez-Hoyos and Jose Alberola-Ila / Lentiviral vector delivery of recombinant small interfering RNA expression cassettes / Ming-Jie Li and John J. Rossi --
Paradigms for conditional expression of RNA interference molecules for use against viral targets / David S. Strayer, Mark Feitelson, Bill Sun, and Alexey A. Matskevich --
High-throughput RNA interference strategies for target discovery and validation by using synthetic short interfering RNAs: functional genomics investigations of biological pathways / Christoph Sachse, Eberhard Krausz, Andrea Kronke, Michael Hannus, Andrew Walsh, Anne Grabner, Dmitriy Ovcharenko, David Dorris, Claude Trudel, Birte Sonnichsen, and Christophe J. Echeverri --
RNA interference In Vivo: toward synthetic small inhibitory RNA-based therapeutics / Antonin de Fougerolles, Muthiah Manoharan, Rachel Meyers and Hans-Peter Vornlocher --
Labeling and characterization of small RNAs associated with the RNA interference effector complex RITs / Andre Verdel and Danesh Moazed --
RNA interference spreading in C. elegans / Robin C. May and Ronald H.A. Plasterk --
Human dicer: purification, properties, and interaction with PAZ PIWI domain proteins / Fabrice A. Kolb, Haidi Zhang, Kasia Jaronczyk, Nasser Tahbaz, Tom C. Hobman and Witold Filipowicz --
Delivery of siRNA and siRNA expression constructs to adult mammals by hydrodynamic intravascular injection / David L. Lewis and Jon A. Wolff --
Analysis of short interfering RNA function in RNA interference by using Drosophila embryo extracts and Schneider cells / Concetta Lipardi, Hye Jung Baek, Qin Wei and Bruce M. Paterson --
Use of RNA polymerase II to transcribe artificial microRNAs / Yan Zeng, Xuezhong Cai, and Bryan R. Cullen --
Adeno-associated virus vectors for short hairpin RNA expression / Dirk Grimm, Kusum Pandey, and Mark A. Kay --
Simple, robust strategies for generating DNA-directed RNA interference constructs / Robert R. Rice, Andrew N. Muirhead, Bruce T. Harrison, Andrew J. Kassianos, Petra L. Sedlak, Narelle J. Maugeri, Peter J. Goss, Jonathan R. Davey, David E. James and Michael W. Graham.
Series Title: Methods in enzymology, v. 392.
Responsibility: edited by David R. Engelke, John J. Rossi.

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