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RNA methodologies : a laboratory guide for isolation and characterization

Author: Robert E Farrell, Jr.
Publisher: Amsterdam ; Boston : Elsevier/Academic Press, ©2005.
Edition/Format:   Print book : English : 3rd edView all editions and formats
Summary:
This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate  Read more...
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Genre/Form: Laboratory manuals
Laboratory Manuals
Manuels de laboratoire
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: Robert E Farrell, Jr.
ISBN: 0122496965 9780122496967
OCLC Number: 57414698
Description: xxv, 767 pages : illustrations ; 23 cm
Contents: RNA and the cellular biochemistry revisited --
Transcription and the organization of eukaryotic genes --
Messenger RNA --
Resilient ribonucleases --
RNA isolation strategies --
The truth about tissues --
Isolation of polyadenylated RNA --
Quality control for RNA preparations --
Dot blot analysis --
Electrophoresis of RNA --
Photodocumentation and image analysis --
Northern analysis --
Nucleic acid probe technology --
Practical nucleic acid hybridization --
Principles of detection --
Quantification of specific mRNAs by nuclease protection --
Analysis of nuclear RNA --
cDNA synthesis --
RT-PCR --
Quantitative PCR techniques --
Transcript subtraction methods --
mRNA differential display --
High-throughput analysis of gene expression --
RNA interference : targeted gene silencing --
Genomes, transcriptomes, proteomes, and bioinformatics --
An RNA Paradigm --
Epilogue : a few pearls of wisdom.
Responsibility: Robert E. Farrell, Jr.

Abstract:

A laboratory guide representing a collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization  Read more...

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"...an exhaustive collection of tried, tested, and optimized protocols for isolation and characterization of RNA."

 
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