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RNA methodologies : laboratory guide for isolation and characterization

Author: Robert E Farrell, Jr.
Publisher: Amsterdam ; Boston : Elsevier/Academic Press, 2010.
Edition/Format:   eBook : Document : English : 4th ed
Summary:
This is the fourth edition of the successful laboratory guide which has translated the rich story of riboneucleic acid for over fifteen years. RNA Methodologies 4e presents the latest collection of tested laboratory protocols for the isolation and characterization of eukaryotic and prokaryotic RNA with greater emphasis on transcript profiling, including quantification issues and elucidation of alternative  Read more...
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Genre/Form: Laboratory Manuals
Electronic books
Laboratory manuals
Additional Physical Format: Print version:
Farrell, Robert E.
RNA methodologies.
Amsterdam ; Boston : Elsevier/Academic Press, 2010
(DLC) 2005041012
(OCoLC)496226598
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Robert E Farrell, Jr.
ISBN: 9780123747273 0123747279 9780080884950 0080884954
OCLC Number: 528609521
Notes: Previous edition: 2005.
Description: 1 online resource (xxiii, 717 pages) : illustrations
Contents: CHAPTER 1: RNA and the Cellular Biochemistry Revisited --
CHAPTER 2: RNA Isolation Strategies --
CHAPTER 3: The Truth about Tissues --
CHAPTER 4: Going Green: RNA and the Molecular Biology of Plants (NEW) --
CHAPTER 5: Isolation of Polyadenylated RNA --
CHAPTER 6: Quality Control for RNA Preparations (EXPANDED) --
CHAPTER 7: Resilient Ribonucleases --
CHAPTER 8: Stringency: Conditions that Influence Nucleic Acid Structure (EXPANDED) --
CHAPTER 9: Electrophoresis of RNA (EXPANDED) --
CHAPTER 10: Photodocumentation and Image Analysis (EXPANDED) --
CHAPTER 11: Northern Analysis --
CHAPTER 12: Nucleic Acid Probe Technology --
CHAPTER 13: Practical Nucleic Acid Hybridization --
CHAPTER 14: Principles of Detection (EXPANDED) --
CHAPTER 15: Quantification of Specific mRNAs by Nuclease Protection --
CHAPTER 16: Analysis of Nuclear RNA (EXPANDED) --
CHAPTER 17: cDNA Synthesis (EXPANDED) --
CHAPTER 18: RT-PCR (NEW) --
CHAPTER 19: Quantitative PCR Techniques --
CHAPTER 20: Functional Genomics and Transcript Profiling (NEW) --
CHAPTER 21: High-Throughput Analysis of Gene Expression --
CHAPTER 22: Non-Array Methods for Global Analysis of Gene Expression (NEW) --
CHAPTER 23: RNA Interference: Targeted Gene Silencing (EXPANDED) --
CHAPTER 24: Genomes, Transcriptomes, Proteomes, and Bioinformatics --
CHAPTER 25: An RNA Paradigm.
Responsibility: Robert E. Farrell Jr.

Abstract:

Presents a collection of tested laboratory protocols for the isolation and characterization of eukaryotic and prokaryotic RNA. This book emphasises on transcript profiling, including quantification  Read more...

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