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RNA turnover in prokaryotes, archae, and organelles

Author: Lynne Maquat; Cecilia M Arraiano
Publisher: Amsterdam ; Boston : Elsevier/Academic Press, ©2008.
Series: Methods in enzymology, v. 447.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
Specific complexes of protein and RNA carry out many essential biological functions, including RNA processing, RNA turnover, RNA folding, as well as the translation of genetic information from mRNA into protein sequences. Messenger RNA (mRNA) decay is now emerging as an important control point and a major contributor to gene expression. Continuing identification of the protein factors and cofactors, and mRNA  Read more...
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Genre/Form: Electronic books
Additional Physical Format: Print version:
RNA turnover in prokaryotes, archae, and organelles.
Amsterdam ; Boston : Elsevier/Academic Press, ©2008
(OCoLC)294859877
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Lynne Maquat; Cecilia M Arraiano
ISBN: 9780080922065 0080922066
OCLC Number: 388032555
Description: 1 online resource (1, 564 pages) : illustrations.
Contents: Analysis of RNA decay, processing, and polyadenylation in Escherichia coli and other prokaryotes / Bijoy K. Mohanty [and others] --
Analyzing the decay of stable RNAs in E. coli / Zhongwei Li and Murray P. Deutscher --
Genomic analysis of mRNA decay in E. coli with DNA microarrays / Pei-Hsun Lin [and others] --
Co-immunopurification of multiprotein complexes containing RNA-degrading enzymes / Agamemnon J. Carpousis [and others] --
PABLO analysis of RNA : 5'-phosphorylation state and 5'-end mapping / Helena Celesnik, Atilio Deana, and Joel G. Belasco --
A proteomic approach to the analysis of RNA degradosome composition in Escherichia coli / Pierluigi Mauri and Gianni Dehò --
New approaches to understanding double-stranded RNA processing by Ribonuclease III : purification and assays of homodimeric and heterodimeric forms of RNase III from bacterial extremophiles and mesophiles / Wenzhao Meng [and others] --
Characterizing ribonucleases in vitro : examples of synergies between biochemical and structural analysis / Cecília Maria Arraiano, Ana Barbas, and Mónica Amblar --
The role of RNA chaperone Hfq in poly(A) metabolism : methods to determine positions, abundance, and lengths of short oligo(A) tails / Philippe Régnier and Eliane Hajnsdorf --
Assaying DEAD-box RNA helicases and their role in mRNA degradation in Escherichia coli / Agamemnon J. Carpousis, Vanessa Khemici, and Leonora Poljak --
Preparation of the Escherichia coli RNase E protein and reconstitution of the RNA degradosome / George A. Mackie [and others] --
Identifying and characterizing substrates of the RNase E/G family of enzymes / Louise Kime, Stefanie S. Jourdan, and Kenneth J. McDowall --
Construction and characterization of E. coli K12 strains in which the transcription of selected genes is desynchronized from translation / Florence Proux and Marc Dreyfus --
Analysis of mRNA decay in Bacillus subtilis / David H. Bechhofer [and others] --
Assay of Bacillus subtilis ribonucleases in vitro / Ciarán Condon [and others] --
Staphylococcus aureus Endoribonuclease III : purification and properties / Clément Chevalier [and others] --
Studying tmRNA-mediated surveillance and nonstop mRNA decay / Thomas Sundermeier [and others] --
Analyses of mRNA destabilization and translational inhibition mediated by Hfq-binding small RNAs / Teppei Morita [and others] --
In vivo and in vitro studies of RNA degrading activities in Archaea / Elena Evguenieva-Hackenberg, Steffen Wagner, and Gabriele Klug --
Expression, reconstitution, and structure of an archaeal RNA degrading exosome / Esben Lorentzen and Elena Conti --
Polyadenylation-mediated RNA degradation in plant mitochondria / Sarah Holec [and others] --
In vivo and in vitro approaches for studying the yeast mitochondrial RNA degradosome complex / Michal Malecki [and others] --
Measuring mRNA decay in human mitochondria / Asuteka Nagao, Narumi Hino-Shigi, and Tsutomu Suzuki --
Detection and characterization of polyadenylated RNA in eukarya, bacteria, archaea, and organelles / Shimyn Slomovic, Victoria Portnoy, and Gadi Schuster --
RNA decay by messenger RNA interferases / Mikkel Christensen-Dalsgaard [and others].
Series Title: Methods in enzymology, v. 447.
Responsibility: edited by Lynne E. Maquat, Cecilia Arraiano.

Abstract:

Specific complexes of protein and RNA carry out many essential biological functions, including RNA processing, RNA turnover, RNA folding, as well as the translation of genetic information from mRNA into protein sequences. Messenger RNA (mRNA) decay is now emerging as an important control point and a major contributor to gene expression. Continuing identification of the protein factors and cofactors, and mRNA instability elements, responsible for mRNA decay allow researchers to build a comprehensive picture of the highly orchestrated processes involved in mRNA decay and its regulation. & lt;br.

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