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RNAi : design and application

Author: Sailen Barik
Publisher: Totowa, N.J. : Humana Press, ©2008.
Series: Methods in molecular biology (Clifton, N.J.), v. 442.
Edition/Format:   Print book : EnglishView all editions and formats
Summary:

In this book, leaders in the field contribute state-of-the-art, easy to follow methods and bench protocols designed for practical, everyday use of RNAi in biological research.

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Genre/Form: Aufsatzsammlung
Laboratory manuals
Laboratory Manuals
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: Sailen Barik
ISBN: 9781588298744 1588298744 9781597451918 1597451916
OCLC Number: 172979420
Description: xiv, 270 pages : illustrations.
Contents: Principles of dicer substrate (D-siRNA) design and function / Mohammed Amarzguioui and John J. Rossi --
Expression, purification, and analysis of recombinant Drosophila dicer-1 and dicer-2 enzymes / Xuecheng Ye and Qinghua Liu --
In vitro RNA cleavage assay for argonaute-family proteins / Keita Miyoshi [and others] --
Identifying siRNA-induced off-targets by microarray analysis / Emily Anderson [and others] --
Hydrodynamic delivery of siRNA in a mouse model of sepsis / Doreen E. Wesche-Soldato [and others] --
Nasal delivery of siRNA / Vira Bitko and Sailen Barik --
RNA interference as a genetic tool in trypanosomes / Vivian Bellofatto and Jennifer B. Palenchar --
Lentivirus-mediated RNA interference in mammalian neurons / Scott Q. Harper and Pedro Gonzalez-Alegre --
Silencing genes by RNA interference in the protozoan parasite Entamoeba histolytica / Carlos F. Solis and Nancy Guillén --
Use of RNAi in C. elegans / Tsuyoshi Ohkumo [and others] --
Application of siRNA against SARS in the rhesus macaque model / Qingquan Tang [and others] --
siRNA and shRNA as anticancer agents in a cervical cancer model / Wenyi Gu, Lisa Putral, and Nigel McMillan --
Identification and expression analysis of small RNAs during development / Toshiaki Watanabe, Hiroshi Imai, and Naojiro Minami --
Screening and identification of virus-encoded RNA silencing suppressors / Sumona Karjee, Mohammad Nurul Islam, and Sunil K. Mukherjee --
Application of RNA interference in functional genomics studies of a social insect / Michael E. Scharf, Xuguo Zhou, and Margaret A. Schwinghammer --
Temporal control of gene silencing by in ovo electroporation / Thomas Baeriswyl, Olivier Mauti, and Esther T. Stoeckli --
Altering flower color in transgenic plants by RNAi-mediated engineering of flavonoid biosynthetic pathway / Yoshikazu Tanaka, Noriko Nakamura, and Junichi Togami --
Transgenic RNA interference in mice / Pumin Zhang.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 442.
Responsibility: edited by Sailen Barik.
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