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RT-PCR protocols

Author: Joe O'Connell, Dr.
Publisher: Totowa, N.J. : Humana Press, ©2002.
Series: Methods in molecular biology (Clifton, N.J.), v. 193.
Edition/Format:   Print book : EnglishView all editions and formats
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Describing the RT-PCR applications, this book contains reproducible protocols for highly sensitive detection and quantification of gene expression, the in situ localization of gene expression in  Read more...

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Genre/Form: Aufsatzsammlung
Laboratory manuals
Laboratory Manuals
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: Joe O'Connell, Dr.
ISBN: 0896038750 9780896038752
OCLC Number: 48957956
Description: xiv, 378 pages : illustrations ; 24 cm.
Contents: Rt-pcr in biomedicine. Opportunities arising from the new accessibility of mrna / J. O'Connell --
The basics of rt-pcr. Some practical considerations / J. O'Connell --
Using the quantitative competitive rt-pcr technique to analyze minute amounts of different mrnas in small tissue samples / S. Greber-Platzer [and others] --
Detection of mrna expression and alternative splicing in a single cell / T. Kumazaki --
Nested rt-pcr. Sensitivity controls are essential to determine the biological significance of detected mrna / T. Goode [and others] --
Quantitative rt-pcr. A review of current methodologies / C. Joyce --
Rapid development of a quantitative-competitive (qc) rt-pcr assay using a composite primer approach / J. O'Connell [and others] --
Quantitation of gene expression by rt-pcr and hplc analysis of pcr products / F. Bachmair, C.G. Huber and G. Daxenbichler --
Time-resolved fluorometric detection of cytokine mrnas amplified by rt-pcr / K. Nieminen [and others] --
Mimic-based rt-pcr quantitation of substance p mrna in human mononuclear phagocytes and lymphocytes / J.P. Lai, S.D. Douglas and W.Z. Ho --
Detection and quantification of the hepatitis c viral genome / L.J. Fanning --
Semi-quantitative detection of hepatitis c virus rna by "real-time" rt-pcr / J.F. Schlaak --
Rt-pcr for the assessment of genetically heterogenous populations of the hepatitis c virus / B. Mullan [and others] --
In situ immuno-pcr. A newly developed method for highly sensitive antigen detection in situ / Y. Cao --
Rt-pcr from laser-capture microdissected samples / T. Crnogorac-Jurcevic, T.O. Nielsen and N.R. Lemoine --
Mycobacterium paratuberculosis detected by nested pcr in intestinal granulomas isolated by lcm in cases of crohn's disease / P. Ryan [and others] --
Rt-pcr-based approaches to generate probes for mrna detection by in situ hybridization / J. O'Connell --
Amplified rna for gene array hybridizations / V.I. Shustova and S.J. Meltzer --
Semi-quantitative determination of differential gene expression in primary tumors and matched metastases by rt-pcr. Comparison with other methods / B. Mann and C. Hanski --
Detection of single nucleotide polymorphisms using a non-isotopic rnase cleavage assay / F. Waldron-Lynch [and others] --
Detection of clonally expanded t-cells by rt-pcr-sscp and nucleotide sequencing of t-cell receptor beta-cdr3 regions / M.S. Kurokawa, K. Nishioka and T. Kato --
Generation of scfv from a phage display mini-library derived from tumor-infiltrating b-cells / N. Gruel [and others] --
Generation of murine scfv intrabodies from b-cell hybridomas / C.H. Nam, S. Moutel and J.L. Teillaud --
Quantitation of mrna levels by rt-pcr in cells purified by facs. Application to peripheral cannabinoid receptors in leukocyte subsets / J. Marchand and P. Carayon --
Detection of anti-sense rna transcripts by anti-sense rt-pcr / M.C. Yeung and A.S. Lau --
Rt-pcr in cdna library construction / V. Healy --
An rt-pcr-based protocol for the rapid generation of large, representative cdna libraries for expression screening / J. O'Connell.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 193.
Responsibility: edited by Joe O'Connell.
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...RT-PCR succeeds in its goal to provide the reader with useful applications relevant to the wider research community. - Clinical Chemistry It is clearly written and can be easily understood by the Read more...

 
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