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SARS- and other coronaviruses : laboratory protocols

Author: David Cavanagh, Ph. D.
Publisher: Totowa, N.J. : Humana, ©2008.
Series: Methods in molecular biology (Clifton, N.J.), v. 454.
Edition/Format:   Print book : EnglishView all editions and formats
Summary:

In 2003, the word "coronavirus" spread across the globe, somewhat further than the virus that sparked the panic. In this book, expert researchers examine these devastating viruses through 23  Read more...

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Genre/Form: Laboratory manuals
Laboratory Manuals
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: David Cavanagh, Ph. D.
ISBN: 9781588298676 1588298671 9781597451819 1597451819
OCLC Number: 192027781
Description: xv, 344 pages : illustrations ; 24 cm.
Contents: A pancoronavirus RT-PCR assay for detection of all known coronaviruses / Leen Vijgen [and others] --
Detection of group 1 coronaviruses in bats using universal coronavirus reverse transcription polymerase chain reactions / Leo L.M. Poon and J.S. Malik Peiris --
Detection and sequence characterization of the 3'-end of coronavirus genomes harboring the highly conserved RNA motif s2m / Christine Monceyron Janassen --
RT-PCR detection of avian coronaviruses of galliform birds (chicken, turkey, pheasant) and in a parrot / Francesca Anne Culver, Paul Britton, and Dave Cavanagh --
Detection of group 2a coronaviruses with emphasis on bovine and wild ruminant strains / Mustafa Hasoksuz, Anastasia Vlasova, and Linda J. Saif --
Detection of SARS coronavirus in humans and animals by conventional and quantitative (real time) reverse transcription polymerase chain reactions / J.S. Malik Peiris and Leo L.M. Poon --
Detection of new viruses by VIDISCA / Krzysztof Pyrc [and others] --
Titration of human coronaviruses, HCoV-229E and HCoV-OC43, by an indirect immunoperoxidase assay / Francine Lambert [and others] --
The preparation of chicken tracheal organ cultures for virus isolation, propagation, and titration / Brenda V. Jones and Ruth M. Hennion --
Isolation and propagation of coronaviruses in embryonated eggs / James S. Guy --
Large-scale preparation of UV-inactivated SARS coronavirus virions for vaccine antigen / Yasuko Tsunetsugu-Yokota --
Purification and electron cryomicroscopy of coronavirus particles / Benjamin W. Neuman [and others] --
Production of coronavirus nonstructural proteins in soluble form for crystallization / Yvonne Piotrowski [and others] --
Generating antibodies to the gene 3 proteins of infectious bronchitis virus / Amanda R. Pendleton and Carolyn E. Machamer --
Establishment and characterization of monoclonal antibodies against SARS coronavirus / Kazuo Ohnishi --
Production of monospecific rabbit antisera recognizing nidovirus proteins / Jessika C. Zevenhoven-Dobbe [and others] --
Manipulation of the coronavirus genome using targeted RNA recombination with interspecies chimeric coronaviruses / Cornelis A.M. de Haan [and others] --
Generation of recombinant coronaviruses using vaccinia virus as the cloning vector and stable cell lines containing coronaviral replicon RNAs / Klara Kristin Eriksson, Divine Makia, and Volker Thiel --
Transient dominant selection for the modification and generation of recombinant infectious bronchitis coronaviruses / Maria Armesto [and others] --
Engineering infectious cDNAs of coronavirus as bacterial artificial chromosomes / Fernando Almazán, Carmen Galán, and Luis Enjuanes --
Systematic assembly and genetic manipulation of the mouse hepatitis virus A59 genome / Eric F. Donaldson, Amy C. Sims, and Ralph S. Baric --
Identification of sugar residues involved in the binding of TGEV to porcine brush border membranes / Christel Schwegmann-Wessels and Georg Herrler --
Pseudotyped vesicular stomatitis virus for analysis of virus entry mediated by SARS coronavirus spike proteins / Shuetsu Fukushi, Rie Watanabe, and Fumihiro Taguchi.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 454.
Responsibility: edited by Dave Cavanagh.

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From the reviews:"The serious nature of coronaviruses became well known with the discovery of the severe acute respiratory syndrome (SARS) virus which spread rapidly with life-threatening Read more...

 
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