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Serial analysis of gene expression (SAGE) : methods and protocols

Author: Kåre Lehmann Nielsen
Publisher: Totowa, N.J. : Humana Press, ©2008.
Series: Methods in molecular biology (Clifton, N.J.), v. 387.
Edition/Format:   Print book : EnglishView all editions and formats
Summary:
"Serial Analysis of Gene Expression (SAGE): Digital Gene Expression Profiling facilitates the introduction of SAGE into the laboratory, and provides a framework for interpreting and comparing data derived from SAGE experiments. Of the several methods of genetic profiling available, only SAGE measures the expression of both known and unknown genes. SAGE studies encompass 50,000 tags and can provide detailed knowledge  Read more...
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Genre/Form: Aufsatzsammlung
Laboratory Manuals
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: Kåre Lehmann Nielsen
ISBN: 1588296768 9781588296764
OCLC Number: 71552470
Description: x, 208 pages : illustrations ; 24 cm.
Contents: SAGE and LongSAGE / Annabeth Laursen Høgh and Kåre Lehmann Nielsen --
Robust-LongSAGE (RL-SAGE) : an improved LongSAGE method for high-throughput transcriptome analysis / Malali Gowda and Guo-Liang Wang --
aRNA LongSAGE : SAGE with antisense RNA / Anna M. Heidenblut --
SuperSAGE / Hideo Matsumura [and others] --
Low-cost-medium throughput Sanger dideoxy sequencing / Kåre Lehmann Nielsen --
DeepSAGE : higher sensitivity and multiplexing of samples using a simpler experimental protocol / Kåre Lehmann Nielsen --
High-resolution, genome-wide mapping of chromatin modifications by GMAT / Tae-Young Roh and Keji Zhao --
5'- and 3'-RACEfrom LongSAGE tags / Kåre Lehmann Nielsen --
Extraction and annotation of SAGE tags using sequence quality values / Jeppe Emmersen --
Correction of technology-related artifacts in serial analysis of gene expression / Viatcheslav R. Akmaev --
Duplicate ditag analysis in LongSAGE / Jeppe Emmersen --
Statistical comparison of two or more SAGE libraries : one tag at a time / Gerben J. Schaaf [and others] --
Scaling of gene expression data allowing the comparison of different gene expression platforms / Fred van Ruissen [and others] --
Clustering analysis of SAGE transcription profiles using a Poisson approach / Haiyan Huang, Li Cai, and Wing H. Wong --
Identifying nonspecific SAGE tags by context of gene expression / Xijin Ge and San Ming Wang.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 387.
Responsibility: edited by Kåre Lehmann Nielsen.
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Abstract:

This book facilitates the introduction of SAGE into the laboratory and provides a framework for interpreting and comparing data derived from SAGE experiments. The SAGE protocols presented are  Read more...

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