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Skeletal Research : an Experimental Approach.

Author: David J Simmons
Publisher: Oxford : Elsevier Science, 1979.
Edition/Format:   eBook : Document : EnglishView all editions and formats
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Skeletal Research: An Experimental Approach.
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Genre/Form: Electronic books
Additional Physical Format: Print version:
Simmons, David J.
Skeletal Research: An Experimental Approach.
Oxford : Elsevier Science, ©1979
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: David J Simmons
ISBN: 9780323141598 0323141595
OCLC Number: 836402164
Notes: II. Hydroxyproline and Proline.
Description: 1 online resource (631 pages)
Contents: Front Cover; Skeletal Research: An Experimental Approach; Copyright Page; Table of Contents; List of Contributors; Preface; PART I: BONE CELLS, CARTILAGE CELLS, AND ORGAN CULTURE; Chapter 1. Isolated Bone Cells; I. Introduction; II. Important Considerations in the Study of Isolated Bone Cells; III. Method of Choice; IV. Characterization of Separated Bone Cells; References; Chapter 2. Organ Culture of Bone; I. Historical Overview; II. Scope of This Chapter; III. Aspects of Methodology; IV. Quantitative Methods for the Study of Bone Resorption in Organ Culture. V. Quantitative Methods for the Study of Bone Formation in Organ CultureVI. Future Uses and Problems with Organ Culture Techniques; References; Chapter 3. Cartilage Cells and Organ Culture; I. Introduction; II. Organ and Expiant Cultures; III. Chondrocyte Cultures; IV. Species Considerations; V. Metabolism; VI. Regulation of Metabolism; References; PART II: CELL AND TISSUE BIOCHEMISTRY; Chapter 4. Studies on the Intermediary Metabolism of Growth Cartilage; I. Introduction; II. Important Considerations in the Use of Tissue Slices. III. Isolation of Mitochondria from Chick Tibial Epiphyseal CartilageReferences; Chapter 5. Partial Purification of Plasma Membranes from Calcified Connective Tissues; I. Introduction; II. Tissue Disruption; III. Particle Separation; IV. Fractionation Markers; V. Epiphyseal Cartilage Plasma Membranes; VI. Rat Calvaria and Osteosarcoma Plasma Membranes; References; Chapter 6. Isolation of Cartilage Matrix Vesicles; I. The Discovery and Function of Matrix Vesicles; II. Methods for Isolation of Cells and Subcellular Particles from Cartilage. III. Method for Isolation of Matrix Vesicles from Epiphyseal CartilageIV. Comments on the Procedure; V. Comparison with Other Modified Methods and Conclusions; References; Chapter 7. Lipid Techniques for Calcified Tissues; I. Introduction; II. Preparation of Tissue Samples; III. Extraction of Tissue Lipids; IV. Processing of Lipid Extracts; V. Separation of Lipid Classes; VI. Lipid Metabolic Studies; References; PART III: BONE AS TISSUE; Chapter 8. Special Histochemical Methods; I. Methods for the Study of Enzymes; II. Immunohistochemical Methods; III. Methods for the Study of Matrix.

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Skeletal Research: An Experimental Approach.

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