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Sphingolipid metabolism and cell signaling. / Part B

Author: Alfred H Merrill; Yusuf A Hannun
Publisher: San Diego (Calif.) : Academic press, 2000.
Series: Methods in enzymology (Print), 312.
Edition/Format:   Print book : EnglishView all editions and formats
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Additional Physical Format: Sphingolipid metabolism and cell signaling. Part B [Ressource électronique] / edited by Alfred H. Merrill, Yusuf A. Hannun
Amsterdam : Elsevier, cop. 2011. (@Methods in enzymology)
(ABES)152090789
Document Type: Book
All Authors / Contributors: Alfred H Merrill; Yusuf A Hannun
ISBN: 0121822133 9780121822132
ISSN:0076-6879
OCLC Number: 490971233
Notes: Une version électronique est accessible aux usagers des établissements qui ont fait l'acquisition.
Description: 1 vol. (XXXVIII-648 p.) : ill. ; 24 cm.
Contents: Section I: Methods for Analyzing Sphingolipids [1]: Analysis of Sphingoid Bases and Sphingoid Base 1-Phosphates by High-Performance Liquid Chromatography [2]: Enzymatic Method for Measurement of Sphingosine 1-Phosphate [3]: Ceramide Mass Analysis by Normal-Phase High-Performance Liquid Chromatography [4]: Quantitative Determination of Ceramide Using Diglyceride Kinase [5]: Analysis of Sphingomyelin, Glucosylceramide, Ceramide, Sphingosine, and Sphingosine 1-Phosphate by Tandem Mass Spectrometry [6]: Analyses of Glycosphingollpids by High-Performance Liquid Chromatography [7]: Sphingolipid Extraction and Analysis by Thin-Layer Chromatography [8]: Extraction and Analysis of Multiple Sphingolipids from a Single Sample [9]: Purification of Sphingolipid Classes by Solid-Phase Extraction with Aminopropyl and Weak Cation Exchanger Cartridges [10]: Ganglioside Analysis by High-Performance Thin-Layer Chromatography [11]: Purification and Analysis of Gangliosides [12]: Thin-Layer Chromatography Blotting Using Polyvinylidene Difluoride Membrane (Far-Eastern Blotting) and Its Applications [13]: Thin-Layer Chromatography Immunostaining [14]: Monoclonal Anti-Glycosphingolipid Antibodies [15]: Immunolocalization of Gangliosides by Light Microscopy Using Anti-Ganglioside Antibodies [16]: Cloud-Point Extraction of Gangliosides using Nonionic Detergent C14EO6 [17]: Analyses of Glycosphingolipids Using Clam, Mercenaria mercenaria, Ceramide Glycanase [18]: Quantitative Analyses of Binding Affinity and Specificity for Glycolipid Receptors by Surface Plasmon Resonance [19]: Use of Circular Dichroism for Assigning Stereochemistry of Sphingosine and Other Long-Chain Bases [20]: Infrared Determination of Conformational Order and Phase Behavior in Ceramides and Stratum Corneum Models [21]: Use of Nuclear Magnetic Resonance Spectroscopy in Evaluation of Ganglioside Structure, Conformation, and Dynamics [22]: Fluorescence Quenching Assay of Sphingolipid/Phospholipid Phase Separation in Model Membranes Section II: Methods for Analyzing Aspects of Sphingolipid Metabolism in Intact Cells [23]: Synthesis of Fluorescent Substrates and Their Application to Study of Sphingolipid Metabolism in Vitro and in Intact Cells [24]: Selection of Mammalian Cell Mutants in Sphingolipid Biosynthesis [25]: Selection of Yeast Mutants in Sphingolipid Metabolism [26]: Fluorescence-Based Selection of Gene-Corrected Hematopoietic Stem and Progenitor Cells Based on Acid Sphingomyelinase Expression [27]: Mammalian Ganglioside Sialidases: Preparation and Activity Assays Section III: Sphingolipid-Protein Interactions and Cellular Targets [28]: Effects of Sphingosine and Other Sphingolipids on Protein Kinase C [29]: Kinetic Analysis of Sphingoid Base Inhibition of Yeast Phosphatidate Phosphatase [30]: Assays of Sphingosine-Dependent Kinase for 14-3-3 Protein [31]: Synthesis and Use of Caged Sphingolipids [32]: Binding of Sphingosine 1-Phosphate to Cell Surface Receptors [33]: Use of Short-Chain Ceramides [34]: Analysis of Ceramide-Activated Protein Phosphatases [35]: Use of Affinity Chromatography and TID-Ceramide Photoaffinity Labeling for Detection of Ceramide-Binding Proteins [36]: Lectin-Mediated Cell Adhesion to Immobilized Glycosphingolipids [37]: Analysis of Glycolipid-Dependent Cell Adhesion Based on Carbohydrate-Carbohydrate Interaction [38]: Analysis of Interactions between Glycosphingolipids and Microbial Toxins [39]: Oxidation of Aglycone of Glycosphingolipids: Serine and Ceramide Acid Precursors for Soluble Glycoconjugates [40]: Separation of Glycosphingolipid-Enriched Microdomains from Caveolar Membrane Characterized by Presence of Caveolin [41]: Reconstitution of Sphingolipid-Cholesterol Plasma Membrane Mlcrodomalns for Studies of Virus-Glycolipid Interactions [42]: Analysis of Ceramides Present in Glycosylphosphatidylinositol Anchored Proteins of Saccharomyces cerevisiae [43]: Preparation of Functionalized Lipid Tubules for Electron Crystallography of Macromolecules Section IV: Sphingolipid Transport and Trafficking [44]: Applications of BODIPY-Sphingolipid Analogs to Study Lipid Traffic and Metabolism in Cells [45]: Using Biotinylated Gangliosides to Study Their Distribution and Traffic in Cells by Immunoelectron Microscopy [46]: Assays for Transmembrane Movement of Sphingolipids Section V: Other Methods [47]: Compilation of Methods Published in Previous Volumes of Methods in Enzymology Author Index Subject Index
Series Title: Methods in enzymology (Print), 312.
Responsibility: edited by Alfred H. Merrill, Yusuf A. Hannun.

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