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Sphingosine-1-Phosphate : methods and protocols

Author: Alice Pébay; Kursad Turksen
Publisher: New York : Humana Press, ©2012.
Series: Methods in molecular biology, 874.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
Sphingosine-1-phosphate is a bioactive lysophospholipid which has become, in recent years, the focus of much research interest as it has widespread developmental and physio-pathological actions, controlling events within the nervous, reproductive, gastrointestinal, vascular, respiratory, and immune systems, in addition to having a prominent role in cancer, early mammalian embryogenesis, and stem cells. In  Read more...
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Genre/Form: Electronic books
Laboratory manuals
Additional Physical Format: Print version:
Sphingosine-1-Phosphate.
New York : Humana Press, ©2012
(DLC) 2012935580
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Alice Pébay; Kursad Turksen
ISBN: 9781617798009 1617798002 1617797995 9781617797996
OCLC Number: 792807416
Language Note: English.
Notes: Includes index.
Description: 1 online resource (xi, 221 pages).
Contents: Biochemical methods for quantifying sphingolipids : ceramide, sphingosine, sphingosine kinase-1 activity, and sphingosine-1-phosphate / Leyre Brizuela and Olivier Cuvillier --
Isoform-selective assays for sphingosine kinase activity / Melissa R. Pitman, Duyen H. Pham, and Stuart M. Pitson --
Quantification of sphingosine-1-phosphate and related sphingolipids by liquid chromatography coupled to tandem mass spectrometry / Constantin Bode and Markus H. Gräler --
Cleanup method for mass spectrometry of sphingosine-1-phosphate in blood and solid tissues using a phosphate capture molecule / Jun-ichi Morishige, Tamotsu Tanaka, and Kiyoshi Satouchi --
Immunohistochemical detection of sphingosine-1-phosphate and sphingosine kinase-1 in human tissue samples / Barbara Visentin, Gary Reynolds, and Roger Sabbadini --
Assessment of sphingosine-1-phosphate activity in biological samples by receptor internalization and adherens junction formation / Hideru Obinata and Timothy Hla --
High-throughput assays to measure intracellular Ca2+ mobilization in cells that express recombinant S1P receptor subtypes / William J. Valentine and Gabor Tigyi --
Quantifying sphingosine-1-phosphate-dependent activation of the RhoGTPases / Matt D. Medlin, Joan M. Taylor, and Christopher P. Mack --
Optimized protocols to analyze sphingosine-1-phosphate signal transduction pathways during acrosomal exocytosis in human sperm / Silvia A. Belmonte and Laila Suhaiman --
Use of intravital microscopy and in vitro chemotaxis assays to study the roles of sphingosine-1-phosphate in bone homeostasis / Taeko Ishii [and others] --
Assessment of sphingosine-1-phosphate receptor expression and associated intracellular signaling cascades in primary cells of the human central nervous system / Veronique E. Miron [and others] --
Sphingosine-1-phosphate signaling in skeletal muscle cells / Elisabetta Meacci, Francesca Bini, and Chiara Battistini --
Maintenance of human embryonic stem cells by sphingosine-1-phosphate and platelet-derived growth factor / Raymond C.B. Wong, Martin F. Pera, and Alice Pébay --
Ceramide and sphingosine-1-phosphate signaling in embryonic stem cell differentiation / Erhard Bieberich --
Sphingosine-1-phosphate signaling in neural progenitors / Phillip Callihan and Shelley B. Hooks --
Utilizing sphingosine-1-phosphate to stimulate sprouting angiogenesis / Shih-Chi Su and Kayla J. Bayless.
Series Title: Methods in molecular biology, 874.
Responsibility: edited by Alice Pébay, Kursad Turksen.

Abstract:

This Methods in Molecular Biology (TM) volume describes in-depth techniques in an array of cell types and with various physiological applications, showcasing the important effects of S1P in  Read more...

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