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Spin labeling : the next millennium

Author: Lawrence J Berliner
Publisher: New York : Kluwer Academic, ©2002.
Series: Biological magnetic resonance, v. 14.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:

) Thecurrent volume, Spin Labeling: The NextMillennium, presents an excellent collection of techniques and applications that evolved during the past decade since the last volume, volume 8 (1989).

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Genre/Form: Electronic books
Additional Physical Format: Print version:
Spin labeling.
New York : Kluwer Academic, ©2002
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Lawrence J Berliner
ISBN: 0306470721 9780306470721 6610206430 9786610206438
OCLC Number: 50175021
Description: 1 online resource (xvii, 423 pages) : illustrations.
Contents: Cover --
Table of Contents --
Introduction: Reflections on the Beginning of the Spin Labeling Technique --
Chapter 1 Analysis of Spin Label Line Shapes with Novel Inhomogeneous Broadening from Different Component Widths: Application to Spatially Disconnected Domains in Membranes --
1. Introduction --
1.1. Inhomogeneous Broadening Due to Unresolved Hyperfine Structure --
1. 2. Inhomogeneous Broadening Due to Different Component Widths . --
2. Theory --
2.1. Sum of Two Spectra with Different Widths --
2.2. Statistical Distribution of Spin Labels --
2.3. Methods to Determine the Existence of a Statistical Distribution of Spin Labels --
2.4. Experimental Considerations --
3. Applications --
3.1. Domain Structure in a Two-Component, Two-Phase Lipid Bilayer System --
3.2. Effect of a Peptide on Domain Structure --
4. Conclusions --
References --
Chapter 2 Progressive Saturation and Saturation Transfer EPR for Measuring Exchange Processes and Proximity Relations in Membranes --
1. Introduction --
1.1. Continuous Wave Saturation --
1. 2. Effective Spin Lattice Relaxation Times --
2. Contributions to Intrinsic Spin-Lattice Relaxation Rates --
2.1. Electron Relaxation Mechanisms --
2.2. Nuclear Relaxation Mechanisms --
3. Paramagnetic Relaxation Enhancement --
3.1. Paramagnetic Enhancement by Heisenberg Exchange --
3.2. Paramagnetic Enhancement by Magnetic Dipole-Dipole Interactions --
4. Spin Lattice Relaxation Enhancement by Exchange Processes --
4.1. Two-Site Exchange --
4.2. Heisenberg Spin Exchange --
5. Continuous Wave Saturation Measurements --
5.1. Progressive Saturation EPR --
5.2. Saturation Transfer EPR --
6. Applications --
6.1. Two-Site Lipid Exchange and Lipid-Protein Interactions --
6.2. Lipid and Protein Collision Frequencies: Translational Diffusion --
6.3. Spin Label Location and Accessibility: Paramagnetic Relaxation --
6.4. Distance Measurements: Dipolar Relaxation --
6.5. Slow Rotational Diffusion and Saturation Studies --
7. Conclusions --
References --
Chapter 3 Comparative Spin Label Spectra at X-Band and W-band --
1. Introduction --
2. Comparative X- and W-Band EPR Spectra of Spin Labels in Isotropic Liquids --
2.1. Solution of Perdeuterated Tempone in Toluene --
2.2. Solution of 3-Doxyl-17 Hydroxy-5 Androstane in O-xylene --
2.3. Resolution Enhancement in High Magnetic Fields: Mixtures of Phenyl-tert-butylnitrone Spin Adducts --
3. Sample Deoxygenation in CW HF EPR Experiments --
4. Comparative X- and W-Band EPR Spectra of Spin-Labeled Phospholipids and Proteins --
4.1. Small Spin Labels in Phospholipid Membranes --
4.2. Doxyl Stearic Acid Labels in Phospholipid Membranes --
4.3. Binding Spin-Labeled Fatty Acids to Bovine Serum Albumin --
4.4. Spin-Labeled Proteins: Immobilized Labels --
4.5. Spin-Labeled Proteins: Mobile Labels --
References --
Chapter 4 Use of Imidazoline Nitroxides in Studies of Chemical Reactions: ESR Measurements of the Concentration and Reactivity of Protons, Thiols, and Nitric Oxide --
1. Introduction --
2. Stable Nitroxides in Proton Exchange Reactions --
2.1. Theoretical Considerations --
2.2. Effect of pH on ESR Spectra of Stable Nitroxides --
2.3.pH-Sensitive Nitroxides in Studies of Proton Exchange Reactions --
2.4. ESR Measurements of the Local Concentration of Protons --
2.5. ESR Studies of Proton.
Series Title: Biological magnetic resonance, v. 14.
Responsibility: edited by Lawrence J. Berliner.

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about a previous volume: `Continues the tradition of high quality that has come to be expected from this series.' Analytical Chemistry

 
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