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Stem cells and aging : methods and protocols

Author: Kursad Turksen
Publisher: New York : Humana Press : Springer, ©2013.
Series: Methods in molecular biology (Clifton, N.J.), v. 976.; Springer protocols (Series)
Edition/Format:   Print book : EnglishView all editions and formats
Summary:
Over time, it has become clear that changes in stem cells do occur during aging, not only in their number but also in their relationship to their microenvironment and their functionality as reflected in changes to their metabolome. Stem Cells and Aging: Methods and Protocols brings together chapters from expert contributors with protocols critical for exploring the biology of stem cell aging, all of which is key for  Read more...
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Genre/Form: Laboratory manuals
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: Kursad Turksen
ISBN: 9781627033169 1627033165 9781627033176 1627033173
OCLC Number: 813948044
Description: xi, 179 pages : illustrations (some color).
Contents: Comprehensive hematopoietic stem cell isolation methods / Kyle Rector, Yi Liu, and Gary Van Zant --
Serial transplantation of bone marrow to test self-renewal capacity of hematopoietic stem cells in vivo / Charusheila Ramkumar, Rachel M. Gerstein, and Hong Zhang --
Mouse hematopoietic stem cell transplantation / Hui Cheng, Paulina H. Liang, and Tao Cheng --
Isolation, characterization, and transplantation of adult liver progenitor cells / Mladen I. Yovchev, Mariana D. Dabeva, and Michael Oertel --
Isolation of muscle-derived stem/progenitor cells based on adhesion characteristics to collagen-coated surfaces / Mitra Lavasani [and others] --
Human myoblasts from skeletal muscle biopsies : in vitro culture preparations for morphological and cytochemical analyses at light and electron microscopy / Manuela Malatesta [and others] --
Cardiac stem cell senescence / Daniela Cesselli [and others] --
Isolation of mesenchymal stem cells from human bone and long-term cultivation under physiologic oxygen conditions / Sebastian Klepsch [and others] --
Methods for assessing effects of Wnt/b-catenin signaling in senescence of mesenchymal stem cells / Hai-jie Wang and Yu-zhen Tan --
Intra-femoral injection of human mesenchymal stem cells / Sindhu T. Mohanty and Ilaria Bellantuono --
Tracking of replicative senescence in mesenchymal stem cells by colony-forming unit frequency / Anne Schellenberg, Hatim Hemeda, and Wolfgang Wagner --
Chromatin immunoprecipitation coupled by quantitative real-time PCR as a tool for analyzing epigenetic regulation of stem cell aging / Seunghee Lee, Ji-Won Jung, and Kyung-Sun Kang --
Quantitative fluorescence in situ hybridization on paraffin embedded tissue / Mario Ricciardi, Mauro Krampera, and Marco Chilosi.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 976.; Springer protocols (Series)
Responsibility: edited by Kursad Turksen.

Abstract:

This Methods in Molecular Biology (TM) book presents protocols critical for exploring the biology of stem cell aging, which is vital for understanding age-related stem cell changes at a basic biology  Read more...

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