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Stress response : methods and protocols

Author: Stephen M Keyse
Publisher: Totowa, NJ : Humana Press, ©2000.
Series: Methods in molecular biology (Clifton, N.J.), v. 99.
Edition/Format:   Print book : EnglishView all editions and formats
Database:WorldCat
Summary:

It is understood that the response of mammalian cells to a wide variety of potentially toxic agents may be linked with many human diseases. In this volume, Stephen Keyse has assembled a diverse  Read more...

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Genre/Form: Laboratory Manuals
Laboratory manuals
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: Stephen M Keyse
ISBN: 0896036111 9780896036116
OCLC Number: 42475447
Description: xvi, 488 pages : illustrations ; 24 cm.
Contents: Identifying and counting protein modifications triggered by nitrosative stress / Prabodh K. Sehajpal, Harry M. Lander --
Determination of carbonyl groups in oxidized proteins / Rodney L. Levine, Nancy Wehr, Joy A. Williams, Earl R. Stadtman, Emily Shacter --
Quantitation of 4-hydroxynonenal protein adducts / Koji Uchida, Earl R. Stadtman --
Detection of oxidative stress in lymphocytes using dichlorodihydrofluorescein diacetate / Cecile M. Krejsa, Gary L. Schieven --
The measurement of protein degradation in response to oxidative stress / Thomas Reinheckel, Tilman Grune, Kelvin J. Davies --
Analysis of the role of the AMP-activated protein kinase in the response to cellular stress / D. Grahame Hardie, Ian P. Salt, Stephen P. Davies --
Detection and activation of stress-responsive tyrosine kinases / Gary L. Schieven --
Detection of DNA-dependent protein kinase in extracts from human and rodent cells / Yamini Achari, Susan P. Lees-Miller --
Expression and assay of recombinant ATM / Yael Ziv, Sharon Banin, Dae-Sik Lim, Christine E. Canman, Michael B. Kastan, Yosef Shiloh --
Detection and purification of a multiprotein kinase complex from mammalian cells : IKK Signalsome / Frank Mercurio, David B. Young, Anthony M. Manning --
Methods to assay stress-activated protein kinases / Ana Cuenda --
Monitoring the activation of stress-activated protein kinases using GAL4 fusion transactivators / Chao-Feng Zheng, Li Xu --
Use of kinase inhibitors to dissect signaling pathways / Ana Cuenda, Dario R. Alessi --
The development and use of phospho-specific antibodies to study protein phosphorylation / Jeremy P. Blaydes, Borek Vojtesek, Graham B. Bloomberg, Ted R. Hupp --
Peptide assay of protein kinases and use of variant peptides to determine recognition motifs / D. Grahame Hardie --
Assaying NF-KB and AP-1 DNA-binding and transcriptional activity / Judith M. Mueller, Heike L. Pahl --
Analysis of the mammalian heat-shock response : inducible gene expression and heat-shock factor activity / Anu Mathew, Yanhong Shi, Caroline Jolly, Richard I. Morimoto --
Approaches to define the involvement of reactive oxygen species and iron in ultraviolet-A inducible gene expression / Charareh Pourzand, Olivier Reelfs, Rex M. Tyrrell --
The human immunodeficiency virus LTR-promoter region as a reporter of stress-induced gene expression / Michael W. Bate, Sushma R. Jassal, David W. Brighty --
SAGE : the serial analysis of gene expression / Jill Powell --
Analysis of differential gene expression using the SABRE enrichment protocol / Daniel J. Lavery, Philippe Fonjallaz, Fabienne Fleury-Olela, Ueli Schibler --
UVB-regulated gene expression in human keratinocytes: analysis by differential display / Harry Frank Abts, Thomas Welss, Kai Breuhahn, Thomas Ruzicka --
Heme oxygenase activity current methods and applications / Stefan W. Ryter, Egil Kvam, Rex M. Tyrrell --
Analysis of molecular chaperone activities using in vitro and in vivo approaches / Brian C. Freeman, Annamieke Michels, Jaewhan Song, Harm H. Kampinga, Richard I. Morimoto --
Analysis of chaperone properties of small Hsp's / Monika Ehrnsperger, Matthias Gaestel, Johannes Buchner --
Analysis of small Hsp phosphorylation / Rainer Benndorf, Katrin Engel, Matthias Gaestel --
Analysis of multisite phosphorylation of the p53 tumor-suppressor protein bytryptic phosphopeptide mappin / David W. Meek, Diane M. Milne --
Development of physiological models to study stress protein responses / Ted R. Hupp.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 99.
Responsibility: edited by Stephen M. Keyse.
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"This handbook will be very helpful in this respect for everybody interested in studies on the effects of stress at the cellular level using biochemical and molecular biology methods." -Cell Biology Read more...

 
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