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Studies of multi-heme cytochromes from Geobacter sulfurreducens

Author: Marianne SchifferP Raj PokkuluriYuri LonderValerie OrshonskyNorma DukeAll authors
Publisher: Washington, D.C. : United States. Dept. of Energy. Office of Science ; Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2004.
Edition/Format:   eBook : Document : Conference publication : National government publication : English
Database:WorldCat
Summary:
The Geobacteraceae family predominates in the reduction of uranium in subsurface environments. We are focusing on the model organism, Geobacter sulfurreducens; its genome contains a large number (>100) of cytochromes c that function in metal reduction pathways. Intensive functional genomics and physiological studies are in progress in Prof. Derek Lovley's laboratory, and the complete genome sequence of this  Read more...
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Material Type: Conference publication, Document, Government publication, National government publication, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Marianne Schiffer; P Raj Pokkuluri; Yuri Londer; Valerie Orshonsky; Norma Duke; Argonne National Lab.; United States. Department of Energy. Office of Science.; United States. Department of Energy. Environmental Management Science Program.; United States. Department of Energy. Office of Scientific and Technical Information.
OCLC Number: 316456584
Notes: Published through the Information Bridge: DOE Scientific and Technical Information.
03/17/2004.
"CONF-NABIR2004-36."
Annual NABIR PI Meeting, March 15-17, 2004, Warrenton, VA.
Schiffer, Marianne; Pokkuluri, P. Raj; Londer, Yuri; Orshonsky, Valerie; Duke, Norma.
Details: Mode of access: World Wide Web.

Abstract:

The Geobacteraceae family predominates in the reduction of uranium in subsurface environments. We are focusing on the model organism, Geobacter sulfurreducens; its genome contains a large number (>100) of cytochromes c that function in metal reduction pathways. Intensive functional genomics and physiological studies are in progress in Prof. Derek Lovley's laboratory, and the complete genome sequence of this organism has been determined by Methe et al. 2003. We are studying cytochromes from the c{sub 7} family that are required for the reduction of Fe(III). Previously, we expressed in E. coli (Londer et al., 2002) and determined the three-dimensional structure at 1.45 {angstrom} resolution (Pokkuluri et al., 2004a) of the three-heme cytochrome c{sub 7} (PpcA, coded by ORF01023) characterized by Lloyd et al., 2003. Further we identified in the G. sulfurreducens genome ORFs for several of its homologs (Pokkuluri et al., 2004a). Four of the ORFs are the same size as PpcA; three other ORFs are polymers of c{sub 7}-type domains, two of which consist of four domains and one of nine domains, that contain 12 and 27 hemes respectively.

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