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T cell protocols : development and activation

Author: Kelly P Kearse
Publisher: Totowa, N.J. : Humana Press, ©2000.
Series: Methods in molecular biology (Clifton, N.J.), v. 134.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Database:WorldCat
Summary:
In T Cell Protocols: Development and Activation, Kelly P. Kearse and a panel of laboratory experts describe the step-by-step execution of a wide variety of basic and specialized techniques for the study of T cell developmental biology and of their subsequent activation. Readily reproducible, the techniques are explained concisely, but in such detail that they are suitable for both the novice and experienced  Read more...
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Genre/Form: Electronic books
Laboratory manuals
Additional Physical Format: T cell protocols
(DLC) 99025100
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Kelly P Kearse
ISBN: 0896038106 9780896038103 1592596827 9781592596829
OCLC Number: 55698102
Description: 1 online resource (xiv, 365 pages) : illustrations.
Contents: pt. 1. Overview: for chapters 2-15 --
Insights into T-cell development using transgenic and knockout mice --
pt. 2. T cell development --
Isolation and characterization of murine early intrathymic precursor populations --
Differentiation of mouse thymocytes in fetal thymus organ culture --
Purification of immature CD4+CD8+ thymyocytes by panning with anti-CD8 antibody --
Transfection and transcription of genes in developing thymocytes --
analysis of CD4/CD8 lineage commitment by pronase treatment and reexpression assay --
Intrahymic delivery of MHC genes using recombinant adenoviruses --
Quantitative analysis of the usage of human T-cell receptor [alpha] and [beta] chain variable regions by reverse dot-blot hybridization --
Flow cytometric detection of cytoplasmic and surface CD3-£ expression in developing T cells --
Assessing apoptosis in developing T cells by flow cytometry --
Isolation of T-cells subsets by magnetic cell sorting (MACS) --
Isolation of T-cell antigens by retrovirus-mediated expression cloning --
Flow cytometric analysis of murine T lymphocytes: a practical guide --
Production and characterization of T cell hybridomas --
pt. 3. Overview: for chapters 17-29 --
Something happens: a brief history of TCR signal transduction --
pt. 4. T-cell activation --
Measurement of phosphatidylinositol (PI) hydrolysis in activated T lymphocytes --
Fluorescence polarization as an early measure of T-lymphocyte stimulation --
Measurement of lymphoproliferation at the single-cell level by flow cytometry --
Digital image analysis of lymphocyte activation --
Induction of T-cell signaling by immobilized integrin ligands --
Cytotoxic T-cell adherence assay (CAA) --
Detecting ubiquitinated T-cell antigen receptor subunits by immunoblotting --
Determination of CD45 tyrosine phosphatase activity in T lymphocytes --
Measurement of protein tyrosine phosphorylation in T-cell subsets by flow cytometry --
Biochemical analysis of activated T lymphocytes: protein phosphorylation and Ras, ERK and JNK activation --
Activation of heterotrimeric GTP-binding proteins upon TCR/CD3 engagement --
Generation of human T helper 1 and T helper 2 subsets form peripheral blood-derived naive CD4+ T cells --
Transcriptional expression analysis of T-cell activation by multiplex meassenger assay (MMA).
Series Title: Methods in molecular biology (Clifton, N.J.), v. 134.
Responsibility: edited by Kelly P. Kearse.

Abstract:

In T Cell Protocols: Development and Activation, Kelly P. Kearse and a panel of laboratory experts describe the step-by-step execution of a wide variety of basic and specialized techniques for the study of T cell developmental biology and of their subsequent activation. Readily reproducible, the techniques are explained concisely, but in such detail that they are suitable for both the novice and experienced investigator. Coverage includes treatment of both fundamental and novel experimental protocols for the analysis of the role T cells play in immune function and in the prevention and cure of human diseases. Practical and timely, T Cell Protocols: Development and Activation offers basic scientists and clinical investigators a valuable compendium of key protocols that will improve skills with existing methods, as well as afford rapid mastery of powerful new techniques.

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