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Telomeres and telomerase : methods and protocols

Author: Songyang Zhou
Publisher: New York : Humana Press ; Springer, [2017] ©2017
Series: Methods in molecular biology (Clifton, N.J.), v. 1587.
Edition/Format:   eBook : Document : English : Third editionView all editions and formats
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Genre/Form: Electronic books
Laboratory manuals
Laboratory Manuals
Additional Physical Format: Print version:
Telomeres and telomerase.
New York : Humana Press, c2017
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Songyang Zhou
ISBN: 9781493968923 1493968920
OCLC Number: 979416171
Description: 1 online resource (xi, 218 pages) : illustrations (some color)
Contents: Introduction to telomeres and telomerase / Zhou Songyang --
Analysis of average telomere length in human telomeric protein knockout cells generated by CRISPR/Cas9 / Jun Xu, Zhou Songyang, Dan Liu, and Hyeung Kim --
Telomere length analysis by quantitative fluorescent in situ hybridization (Q-FISH) / Isabelle Ourliac-Garnier and Arturo Londoño-Vallejo --
Telomere strand-specific length analysis by fluorescent in situ hybridization (Q-CO-FISH) / Isabelle Ourliac-Garnier and Arturo Londoño-Vallejo --
Telomere G-rich overhang length measurement : DSN method / Yong Zhao, Jerry W. Shay, and Woodring E. Wright --
Telomere G-overhang length measurement method 2 : G-tail telomere HPA / Hidetoshi Tahara --
Telomere terminal G/C strand synthesis : measuring telomerase action and C-rich fill-in / Yong Zhao, Jerry W. Shay, and Woodring E. Wright --
Analysis of yeast telomerase by primer extension assays / Min Hsu and Neal F. Lue --
Assessing telomerase activities in mammalian cells using the quantitative PCR-based telomeric repeat amplification protocol (qTRAP) / Shuai Jiang, Mengfan Tang, Huawei Xin, and Junjiu Huang --
Telomeres and nextgen CO-FISH : directional genomic hybridization (Telo-dGH) / Miles J. McKenna, Erin Robinson, Edwin H. Goodwin, Michael N. Cornforth, and Susan M. Bailey --
Visualization of human telomerase localization by fluorescence microscopy techniques / Eladio Abreu, Rebecca M. Terns, and Michael P. Terns --
Cytogenetic analysis of telomere dysfunction / Rekha Rai, Asha S. Multani, and Sandy Chang --
Probing the telomere damage Response / Rekha Rai and Sandy Chang --
Induction of site-specific oxidative damage at telomeres by killerred-fused shelretin proteins / Rong Tan and Li Lan --
Using protein-fragment complementation assays (PCA) and peptide arrays to study telomeric protein-protein interactions / Wenbin Ma, Ok-hee Lee, Hyeung Kim, and Zhou Songyang --
In vitro preparation and crystallization of vertebrate telomerase subunits / Jing Huang, Christopher J. Bley, Dustin P. Rand, Julian J.L. Chen, and Ming Lei --
Human telomeric G-quadruplex structures and G-quadruplex-interactive compounds / Clement Lin and Danzhou Yang --
Analysis of telomere-homologous DNA with different conformations using 2D agarose electrophoresis and in-gel hybridization / Zepeng Zhang, Qian Hu, and Yong Zhao --
Analysis of telomere proteins by chromatin immunoprecipitation (ChIP) / Feng Liu, Xuyang Feng, and Wenbin Ma.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 1587.
Responsibility: edited by Zhou Songyang.

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