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Test No. 302C: Inherent Biodegradability: Modified MITI Test (II)

Author: Organisation for Economic Co-operation and Development.
Publisher: [Paris] : OECD Publishing, 2009.
Series: OECD Guidelines for the Testing of Chemicals, Section 3: Degradation and Accumulation
Edition/Format:   eBook : Document : International government publication : EnglishView all editions and formats
Summary:
This Test Guideline describes the modified MITI test (II). This test permits the measurement of the Biochemical Oxygen Demand (BOD) and the analysis of residual chemicals in order to evaluate the inherent biodegradability of chemical substances which have been found by the Standard MITI Method (I) to be low degradable. An automated closed-system oxygen consumption measuring apparatus (BOD-meter) is used. Chemicals  Read more...
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Details

Material Type: Document, Government publication, International government publication, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Organisation for Economic Co-operation and Development.
ISBN: 9789264070400 9264070400
OCLC Number: 704441941
Language Note: English.
Description: 1 online resource (21 pages) : illustrations.
Series Title: OECD Guidelines for the Testing of Chemicals, Section 3: Degradation and Accumulation
Responsibility: Organisation for Economic Co-operation and Development.

Abstract:

This Test Guideline describes the modified MITI test (II). This test permits the measurement of the Biochemical Oxygen Demand (BOD) and the analysis of residual chemicals in order to evaluate the inherent biodegradability of chemical substances which have been found by the Standard MITI Method (I) to be low degradable. An automated closed-system oxygen consumption measuring apparatus (BOD-meter) is used. Chemicals to be tested are inoculated in the testing vessels (six bottles with different quantities of test chemical) with micro-organisms. In order to check the activity of the inoculum, the use of control substances (aniline, sodium acetate or sodium benzoate) is desirable. During the test period, the BOD is measured continuously. Biodegradability is calculated on the basis of BOD and supplemental chemical analysis, such as measurement of the dissolved organic carbon concentration, concentration of residual chemicals, etc. The BOD curve is obtained continuously and automatically for 14 to 28 days. After the 14 to 28 days of testing, pH, residual chemicals and intermediates in the testing vessels are analysed.

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