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Test No. 431: In vitro skin corrosion: reconstructed human epidermis (RHE) test method

Author: Organisation for Economic Co-operation and Development.
Publisher: Paris : OECD Publishing, 2016.
Series: OECD Guidelines for the Testing of Chemicals, Section 4.
Edition/Format:   eBook : Document : International government publication : EnglishView all editions and formats
Summary:
The test described in this Test Guideline allows the identification of corrosive chemical substances and mixtures and it enables the identification of non-corrosive substances and mixtures when supported by a weight of evidence determination using other existing information. The test protocol may also provide an indication of the distinction between severe and less severe skin corrosives. This Test Guideline does  Read more...
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Details

Material Type: Document, Government publication, International government publication, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Organisation for Economic Co-operation and Development.
ISBN: 9789264264618 9264264612
OCLC Number: 961873228
Description: 1 online resource (26 pages).
Series Title: OECD Guidelines for the Testing of Chemicals, Section 4.
Responsibility: Organisation for Economic Co-operation and Development.

Abstract:

The test described in this Test Guideline allows the identification of corrosive chemical substances and mixtures and it enables the identification of non-corrosive substances and mixtures when supported by a weight of evidence determination using other existing information. The test protocol may also provide an indication of the distinction between severe and less severe skin corrosives. This Test Guideline does not require the use of live animals or animal tissue for the assessment of skin corrosivity. The test material (solid or liquid) is applied uniformly and topically to a three-dimensional human skin model, comprising at least a reconstructed epidermis with a functional stratum corneum. Two tissue replicates are used for each treatment (exposure time), and for controls. Corrosive materials are identified by their ability to produce a decrease in cell viability below defined threshold levels at specified exposure periods. Coloured chemicals can also be tested by used of an HPLC procedure. The principle of the human skin model assay is based on the hypothesis that corrosive chemicals are able to penetrate the stratum corneum by diffusion or erosion, and are cytotoxic to the underlying cell layers.

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